Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model
Abstract Objective This study aimed to identify significant differences in cochlea microvessel size between a diabetic mouse model (db/db) and normal mice using three‐dimensional (3D) quantitative analysis. Methods Six control heterozygote db/+ as well as 18 male B6/BKS(D)‐Leprdb/J (db/db) mice aged...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-10-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.917 |
| _version_ | 1811238316070141952 |
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| author | Young Joon Seo Dae Bo Shim Arazu Sharif Zeke Samson Ryu Takechi Daniel Brown |
| author_facet | Young Joon Seo Dae Bo Shim Arazu Sharif Zeke Samson Ryu Takechi Daniel Brown |
| author_sort | Young Joon Seo |
| collection | DOAJ |
| description | Abstract Objective This study aimed to identify significant differences in cochlea microvessel size between a diabetic mouse model (db/db) and normal mice using three‐dimensional (3D) quantitative analysis. Methods Six control heterozygote db/+ as well as 18 male B6/BKS(D)‐Leprdb/J (db/db) mice aged 14 (n = 9) and 28 (n = 9) weeks were examined. After clearing the cochlea, we reconstructed the 3D volumes of the spiral modiolar artery (SMA) in the cochlea using light‐sheet microscopy and analyzed vessel wall thickness, cross‐sectional area, short and long diameter, and vessel height. Results The average SMA‐wall thickness in the db/db‐mouse group (3.418 ± 0.328 μm) was greater than that in the control group (2.388 ± 0.411 μm). The average cross‐sectional outer area, short diameter, and long diameter of the SMA in db/db mice were significantly larger than those in control mice (all p < 0.001). The cross‐sectional areas increased with age (control: 221.782 ± 121.230 μm, 14 weeks; 294.378 ± 151.008 μm, and 28 weeks; 312.925 ± 147.943 μm). Conclusion The db/db mice had thicker and larger proximal‐SMA vessel walls and diameters than control mice, respectively, thus potentially inducing increased blood viscosity or vascular insufficiency and aggravating hearing loss in type 2 diabetes mellitus. Level of Evidence IIb |
| first_indexed | 2024-04-12T12:39:39Z |
| format | Article |
| id | doaj.art-9612ce5b7e294a98b3f62bf116a5369f |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-04-12T12:39:39Z |
| publishDate | 2022-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-9612ce5b7e294a98b3f62bf116a5369f2022-12-22T03:32:49ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-10-01751568157410.1002/lio2.917Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse modelYoung Joon Seo0Dae Bo Shim1Arazu Sharif2Zeke Samson3Ryu Takechi4Daniel Brown5Department of Otorhinolaryngology Yonsei University Wonju College of Medicine Wonju South KoreaDepartment of Otorhinolaryngology Myongji Hospital, Hanyang University College of MedicineCurtin Health Innovation Research Institute, Curtin University Bentley WA AustraliaFaculty of Health sciences Curtin University Bentley WA AustraliaCurtin Health Innovation Research Institute, Curtin University Bentley WA AustraliaFaculty of Health sciences Curtin University Bentley WA AustraliaAbstract Objective This study aimed to identify significant differences in cochlea microvessel size between a diabetic mouse model (db/db) and normal mice using three‐dimensional (3D) quantitative analysis. Methods Six control heterozygote db/+ as well as 18 male B6/BKS(D)‐Leprdb/J (db/db) mice aged 14 (n = 9) and 28 (n = 9) weeks were examined. After clearing the cochlea, we reconstructed the 3D volumes of the spiral modiolar artery (SMA) in the cochlea using light‐sheet microscopy and analyzed vessel wall thickness, cross‐sectional area, short and long diameter, and vessel height. Results The average SMA‐wall thickness in the db/db‐mouse group (3.418 ± 0.328 μm) was greater than that in the control group (2.388 ± 0.411 μm). The average cross‐sectional outer area, short diameter, and long diameter of the SMA in db/db mice were significantly larger than those in control mice (all p < 0.001). The cross‐sectional areas increased with age (control: 221.782 ± 121.230 μm, 14 weeks; 294.378 ± 151.008 μm, and 28 weeks; 312.925 ± 147.943 μm). Conclusion The db/db mice had thicker and larger proximal‐SMA vessel walls and diameters than control mice, respectively, thus potentially inducing increased blood viscosity or vascular insufficiency and aggravating hearing loss in type 2 diabetes mellitus. Level of Evidence IIbhttps://doi.org/10.1002/lio2.917db/db micediabetes mellitushearing losslight sheet microscopyspiral modiolar artery |
| spellingShingle | Young Joon Seo Dae Bo Shim Arazu Sharif Zeke Samson Ryu Takechi Daniel Brown Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model Laryngoscope Investigative Otolaryngology db/db mice diabetes mellitus hearing loss light sheet microscopy spiral modiolar artery |
| title | Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| title_full | Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| title_fullStr | Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| title_full_unstemmed | Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| title_short | Circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| title_sort | circulatory disturbance of the cochlear spiral modiolar artery in a type 2 diabetic mouse model |
| topic | db/db mice diabetes mellitus hearing loss light sheet microscopy spiral modiolar artery |
| url | https://doi.org/10.1002/lio2.917 |
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