Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments
Ca<sup>2+</sup>-<i>ATPases</i> have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (<i>Musa acuminata</i>) Ca<sup>2+</sup>-<i>ATPases</i> are very limited. In...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/19/11914 |
| _version_ | 1797478778201440256 |
|---|---|
| author | Ronghui Ma Na Tian Jiashui Wang Minlei Fan Bin Wang Pengyan Qu Shiyao Xu Yanbing Xu Chunzhen Cheng Peitao Lü |
| author_facet | Ronghui Ma Na Tian Jiashui Wang Minlei Fan Bin Wang Pengyan Qu Shiyao Xu Yanbing Xu Chunzhen Cheng Peitao Lü |
| author_sort | Ronghui Ma |
| collection | DOAJ |
| description | Ca<sup>2+</sup>-<i>ATPases</i> have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (<i>Musa acuminata</i>) Ca<sup>2+</sup>-<i>ATPases</i> are very limited. In this study, we identified 18 Ca<sup>2+</sup>-<i>ATPase</i> genes from banana, including 6 P-IIA or ER (Endoplasmic Reticulum) type Ca<sup>2+</sup>-<i>ATPases</i> (<i>MaEACs</i>) and 12 P-IIB or Auto-Inhibited Ca<sup>2+</sup>-<i>ATPases</i> (<i>MaACAs</i>). The MaEACs and MaACAs could be further classified into two and three subfamilies, respectively. This classification is well supported by their gene structures, which are encoded by protein motif distributions. The banana Ca<sup>2+</sup>-ATPases were all predicted to be plasma membrane-located. The promoter regions of banana Ca<sup>2+</sup>-<i>ATPases</i> contain many <i>cis</i>-acting elements and transcription factor binding sites (TFBS). A gene expression analysis showed that banana Ca<sup>2+</sup>-<i>ATPases</i> were differentially expressed in different organs. By investigating their expression patterns in banana roots under different concentrations of Ca<sup>2+</sup> treatments, we found that most banana Ca<sup>2+</sup>-<i>ATPase</i> members were highly expressed under 4 mM and 2 mM Ca<sup>2+</sup> treatments, but their expression decreased under 1 mM and 0 mM Ca<sup>2+</sup> treatments, suggesting that their downregulation might be closely related to reduced Ca accumulation and retarded growth under low Ca<sup>2+</sup> and Ca<sup>2+</sup> deficiency conditions. Our study will contribute to the understanding of the roles of Ca<sup>2+</sup>-<i>ATPases</i> in banana growth and Ca management. |
| first_indexed | 2024-03-09T21:37:29Z |
| format | Article |
| id | doaj.art-9613aa6494484f05995a8ec9b3ba8826 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:37:29Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9613aa6494484f05995a8ec9b3ba88262023-11-23T20:41:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191191410.3390/ijms231911914Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> TreatmentsRonghui Ma0Na Tian1Jiashui Wang2Minlei Fan3Bin Wang4Pengyan Qu5Shiyao Xu6Yanbing Xu7Chunzhen Cheng8Peitao Lü9College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Genetic Improvement of Bananas, Hainan Province, Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Horticulture, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCa<sup>2+</sup>-<i>ATPases</i> have been confirmed to play very important roles in plant growth and development and in stress responses. However, studies on banana (<i>Musa acuminata</i>) Ca<sup>2+</sup>-<i>ATPases</i> are very limited. In this study, we identified 18 Ca<sup>2+</sup>-<i>ATPase</i> genes from banana, including 6 P-IIA or ER (Endoplasmic Reticulum) type Ca<sup>2+</sup>-<i>ATPases</i> (<i>MaEACs</i>) and 12 P-IIB or Auto-Inhibited Ca<sup>2+</sup>-<i>ATPases</i> (<i>MaACAs</i>). The MaEACs and MaACAs could be further classified into two and three subfamilies, respectively. This classification is well supported by their gene structures, which are encoded by protein motif distributions. The banana Ca<sup>2+</sup>-ATPases were all predicted to be plasma membrane-located. The promoter regions of banana Ca<sup>2+</sup>-<i>ATPases</i> contain many <i>cis</i>-acting elements and transcription factor binding sites (TFBS). A gene expression analysis showed that banana Ca<sup>2+</sup>-<i>ATPases</i> were differentially expressed in different organs. By investigating their expression patterns in banana roots under different concentrations of Ca<sup>2+</sup> treatments, we found that most banana Ca<sup>2+</sup>-<i>ATPase</i> members were highly expressed under 4 mM and 2 mM Ca<sup>2+</sup> treatments, but their expression decreased under 1 mM and 0 mM Ca<sup>2+</sup> treatments, suggesting that their downregulation might be closely related to reduced Ca accumulation and retarded growth under low Ca<sup>2+</sup> and Ca<sup>2+</sup> deficiency conditions. Our study will contribute to the understanding of the roles of Ca<sup>2+</sup>-<i>ATPases</i> in banana growth and Ca management.https://www.mdpi.com/1422-0067/23/19/11914bananaCa<sup>2+</sup>-ATPasegene expressionCa<sup>2+</sup> deficiencygene family |
| spellingShingle | Ronghui Ma Na Tian Jiashui Wang Minlei Fan Bin Wang Pengyan Qu Shiyao Xu Yanbing Xu Chunzhen Cheng Peitao Lü Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments International Journal of Molecular Sciences banana Ca<sup>2+</sup>-ATPase gene expression Ca<sup>2+</sup> deficiency gene family |
| title | Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments |
| title_full | Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments |
| title_fullStr | Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments |
| title_full_unstemmed | Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments |
| title_short | Genome-Wide Identification and Characterization of Banana Ca<sup>2+</sup>-<i>ATPase</i> Genes and Expression Analysis under Different Concentrations of Ca<sup>2+</sup> Treatments |
| title_sort | genome wide identification and characterization of banana ca sup 2 sup i atpase i genes and expression analysis under different concentrations of ca sup 2 sup treatments |
| topic | banana Ca<sup>2+</sup>-ATPase gene expression Ca<sup>2+</sup> deficiency gene family |
| url | https://www.mdpi.com/1422-0067/23/19/11914 |
| work_keys_str_mv | AT ronghuima genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT natian genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT jiashuiwang genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT minleifan genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT binwang genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT pengyanqu genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT shiyaoxu genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT yanbingxu genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT chunzhencheng genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments AT peitaolu genomewideidentificationandcharacterizationofbananacasup2supiatpaseigenesandexpressionanalysisunderdifferentconcentrationsofcasup2suptreatments |