Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China
Enterocytozoon bieneusi is a widely distributed human and animal pathogen. However, few data are available on the distribution of E. bieneusi genotypes in racehorses. In this study, 621 fecal specimens were collected from racehorses at 17 equestrian clubs in 15 Chinese cities. E. bieneusi was detect...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.01920/full |
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| author | Aiyun Zhao Dongfang Li Zilin Wei Ying Zhang Yushi Peng Yixuan Zhu Meng Qi Longxian Zhang |
| author_facet | Aiyun Zhao Dongfang Li Zilin Wei Ying Zhang Yushi Peng Yixuan Zhu Meng Qi Longxian Zhang |
| author_sort | Aiyun Zhao |
| collection | DOAJ |
| description | Enterocytozoon bieneusi is a widely distributed human and animal pathogen. However, few data are available on the distribution of E. bieneusi genotypes in racehorses. In this study, 621 fecal specimens were collected from racehorses at 17 equestrian clubs in 15 Chinese cities. E. bieneusi was detected via nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) gene. The overall infection rate of E. bieneusi was 4.8% (30/621). Statistically significant differences were found in the prevalence of this parasite among the equestrian clubs (χ2 = 78.464, df = 16, p < 0.01) and age groups (χ2 = 23.686, df = 1, p < 0.01), but no sex bias was found among the racehorses for the E. bieneusi infections (χ2 = 1.407, df = 2, p > 0.05). Ten E. bieneusi genotypes were identified, including seven known genotypes (EbpC, EbpA, Peru6, horse1, horse2, CAF1, and TypeIV) and three novel genotypes (HBH-1, SXH-1, and BJH-1). Phylogenetic analysis showed that EbpC, EbpA, Peru6, horse2, CAF1, TypeIV, BJH-1, and SXH-1 belonged to Group 1 of E. bieneusi, HBH-1 belonged to Group 2, and horse2 belonged to Group 6. Our findings advance the current knowledge of E. bieneusi prevalence and genotypes in racehorses in China. |
| first_indexed | 2024-12-14T10:53:29Z |
| format | Article |
| id | doaj.art-96172ada88e9433794c39fdc04850ae6 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T10:53:29Z |
| publishDate | 2019-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-96172ada88e9433794c39fdc04850ae62022-12-21T23:05:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-08-011010.3389/fmicb.2019.01920473789Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in ChinaAiyun Zhao0Dongfang Li1Zilin Wei2Ying Zhang3Yushi Peng4Yixuan Zhu5Meng Qi6Longxian Zhang7College of Animal Science, Tarim University, Alar, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaCollege of Animal Science, Tarim University, Alar, ChinaCollege of Animal Science, Tarim University, Alar, ChinaEquivets, Beijing, ChinaCollege of Animal Science, Tarim University, Alar, ChinaCollege of Animal Science, Tarim University, Alar, ChinaCollege of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaEnterocytozoon bieneusi is a widely distributed human and animal pathogen. However, few data are available on the distribution of E. bieneusi genotypes in racehorses. In this study, 621 fecal specimens were collected from racehorses at 17 equestrian clubs in 15 Chinese cities. E. bieneusi was detected via nested polymerase chain reaction (PCR) amplification of the internal transcribed spacer (ITS) gene. The overall infection rate of E. bieneusi was 4.8% (30/621). Statistically significant differences were found in the prevalence of this parasite among the equestrian clubs (χ2 = 78.464, df = 16, p < 0.01) and age groups (χ2 = 23.686, df = 1, p < 0.01), but no sex bias was found among the racehorses for the E. bieneusi infections (χ2 = 1.407, df = 2, p > 0.05). Ten E. bieneusi genotypes were identified, including seven known genotypes (EbpC, EbpA, Peru6, horse1, horse2, CAF1, and TypeIV) and three novel genotypes (HBH-1, SXH-1, and BJH-1). Phylogenetic analysis showed that EbpC, EbpA, Peru6, horse2, CAF1, TypeIV, BJH-1, and SXH-1 belonged to Group 1 of E. bieneusi, HBH-1 belonged to Group 2, and horse2 belonged to Group 6. Our findings advance the current knowledge of E. bieneusi prevalence and genotypes in racehorses in China.https://www.frontiersin.org/article/10.3389/fmicb.2019.01920/fullEnterocytozoon bieneusiracehorseprevalencegenotypezoonotic |
| spellingShingle | Aiyun Zhao Dongfang Li Zilin Wei Ying Zhang Yushi Peng Yixuan Zhu Meng Qi Longxian Zhang Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China Frontiers in Microbiology Enterocytozoon bieneusi racehorse prevalence genotype zoonotic |
| title | Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China |
| title_full | Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China |
| title_fullStr | Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China |
| title_full_unstemmed | Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China |
| title_short | Molecular Detection and Genotyping of Enterocytozoon bieneusi in Racehorses in China |
| title_sort | molecular detection and genotyping of enterocytozoon bieneusi in racehorses in china |
| topic | Enterocytozoon bieneusi racehorse prevalence genotype zoonotic |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.01920/full |
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