COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS
The aim of the study was to compare the effects of detonation nanodiamond preparations from different batches cleaned from impurities by diverse methods of chemical treatment on the membrane potential and glutamate transport characteristics of rat brain nerve terminals. The size of nanodiamond pa...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2016-12-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/6_2016/galkin_6_2016.pdf |
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| author | M. A. Galkin О. Yu. Chunihin A. O. Pastukhov R. V. Sivko O. V. Leshchenko O. O. Bochechka N. G. Pozdnyakova Т. O. Borisova |
| author_facet | M. A. Galkin О. Yu. Chunihin A. O. Pastukhov R. V. Sivko O. V. Leshchenko O. O. Bochechka N. G. Pozdnyakova Т. O. Borisova |
| author_sort | M. A. Galkin |
| collection | DOAJ |
| description | The aim of the study was to compare the effects of detonation nanodiamond preparations from different batches cleaned from impurities by diverse methods of chemical treatment on the membrane potential and glutamate transport characteristics of rat brain nerve terminals.
The size of nanodiamond particles vary from 10–20 nm to 10 μm. There are carbonyl, hydroxyl and carboxyl functional groups on the surface of the particles. Physical-chemical properties such as a magnetic susceptibility and the amount of incombustible residue in samples of detonation nanodia-mond vary depending on the synthesis regime and the method of chemical cleaning of the product and therefore, the neuroactive properties of nanodiamonds from different batches can be different.
It was shown by dynamic light scattering analysis that nanodiamond preparations from different batches treated by diverse technologies of chemical treatment had varied average size of particles and distribution of particles by size. Nanodiamond preparations from different batches changed the plasma membrane potential and caused membrane depolarization of nerve terminals. Analysis of the effects of nanodiamonds on transporter-mediated L-[14C]glutamate uptake by nerve terminals also revealed that all studied nanodiamond preparations decreased abovementioned parameter. Therefore, detonation nanodiamonds from different batches have similar principal effects on functional state of nerve terminals, however variability in their physical and chemical properties is associated with diverse strength of these effects. |
| first_indexed | 2024-03-12T11:20:59Z |
| format | Article |
| id | doaj.art-961b36b1f8b04ed9ac8fc70827577555 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T11:20:59Z |
| publishDate | 2016-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-961b36b1f8b04ed9ac8fc708275775552023-09-02T00:42:20ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2016-12-0196283810.15407/biotech9.06.028COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALSM. A. Galkin0О. Yu. Chunihin 1A. O. Pastukhov 2 R. V. Sivko3O. V. Leshchenko4O. O. Bochechka5N. G. Pozdnyakova6Т. O. Borisova7 Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Palladin Institute of Biochemistry of the National Academy of Sciences of UkraineBakul Institute for Superhard Materials of the National Academy of Sciences of Ukraine, Bakul Institute for Superhard Materials of the National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine Palladin Institute of Biochemistry of the National Academy of Sciences of UkraineThe aim of the study was to compare the effects of detonation nanodiamond preparations from different batches cleaned from impurities by diverse methods of chemical treatment on the membrane potential and glutamate transport characteristics of rat brain nerve terminals. The size of nanodiamond particles vary from 10–20 nm to 10 μm. There are carbonyl, hydroxyl and carboxyl functional groups on the surface of the particles. Physical-chemical properties such as a magnetic susceptibility and the amount of incombustible residue in samples of detonation nanodia-mond vary depending on the synthesis regime and the method of chemical cleaning of the product and therefore, the neuroactive properties of nanodiamonds from different batches can be different. It was shown by dynamic light scattering analysis that nanodiamond preparations from different batches treated by diverse technologies of chemical treatment had varied average size of particles and distribution of particles by size. Nanodiamond preparations from different batches changed the plasma membrane potential and caused membrane depolarization of nerve terminals. Analysis of the effects of nanodiamonds on transporter-mediated L-[14C]glutamate uptake by nerve terminals also revealed that all studied nanodiamond preparations decreased abovementioned parameter. Therefore, detonation nanodiamonds from different batches have similar principal effects on functional state of nerve terminals, however variability in their physical and chemical properties is associated with diverse strength of these effects.http://biotechnology.kiev.ua/images/storage/6_2016/galkin_6_2016.pdfnanodiamondglutamateNa+-dependent uptakebrain nerve terminals |
| spellingShingle | M. A. Galkin О. Yu. Chunihin A. O. Pastukhov R. V. Sivko O. V. Leshchenko O. O. Bochechka N. G. Pozdnyakova Т. O. Borisova COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS Biotechnologia Acta nanodiamond glutamate Na+-dependent uptake brain nerve terminals |
| title | COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS |
| title_full | COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS |
| title_fullStr | COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS |
| title_full_unstemmed | COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS |
| title_short | COMPARATIVE STUDY OF THE EFFECTS OF DETONATION NANODIAMONDS WITH VARIED PROPERTIES ON FUNCTIONAL STATE OF BRAIN NERVE TERMINALS |
| title_sort | comparative study of the effects of detonation nanodiamonds with varied properties on functional state of brain nerve terminals |
| topic | nanodiamond glutamate Na+-dependent uptake brain nerve terminals |
| url | http://biotechnology.kiev.ua/images/storage/6_2016/galkin_6_2016.pdf |
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