Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants
<i>Allochrusa gypsophiloides</i> is a rare Central Asian species, a super-producer of triterpene saponins with pharmacological and technical value. In this work, a comparative evaluation of the in vitro culture of adventitious roots (ARs), in vitro adventitious microshoots (ASs), natural...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/20/3521 |
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| author | Valentina K. Mursaliyeva Balaussa T. Sarsenbek Gulnara T. Dzhakibaeva Tlek M. Mukhanov Ramazan Mammadov |
| author_facet | Valentina K. Mursaliyeva Balaussa T. Sarsenbek Gulnara T. Dzhakibaeva Tlek M. Mukhanov Ramazan Mammadov |
| author_sort | Valentina K. Mursaliyeva |
| collection | DOAJ |
| description | <i>Allochrusa gypsophiloides</i> is a rare Central Asian species, a super-producer of triterpene saponins with pharmacological and technical value. In this work, a comparative evaluation of the in vitro culture of adventitious roots (ARs), in vitro adventitious microshoots (ASs), natural roots and aboveground parts of wild plants from Kazakhstan to define the total saponin (TS), phenol (TP) and flavonoid (TF) content, as well as antioxidant (AOA) and antimicrobial activity, is presented for the first time. In the AR culture, growth index (GI), TS, TP and TF were evaluated on days 25, 45 and 60 of cultivation on ½ MS medium without (control) and with auxin application. It was found out that TS and TF were higher in the in vitro AR culture. The amount of TP and TF are higher in the aerial part of vegetative plants with maximum AOA. The concentration of the extract required to inhibit 50% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical formation (ICO<sub>50</sub>) in extracts from natural material negatively correlated with TS, TP, TF and in the in vitro AR culture with TF. Control extracts from the in vitro AR culture with high TS levels showed growth-inhibitory activity against <i>S. thermophillus</i>, <i>S. cerevisiae</i> and <i>C. albicans</i>. The influence shares of medium composition factor, cultivation duration factor and their interaction with GI, TS, TP and TF were determined. The in vitro AR culture is promising for obtaining triterpene saponins TSR with high antibacterial and antifungal activity, and the in vitro ASs culture—for shoot multiplication with antioxidant properties. |
| first_indexed | 2024-03-10T20:57:46Z |
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| id | doaj.art-961ba79b13594aebb019984e4712e618 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:57:46Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-961ba79b13594aebb019984e4712e6182023-11-19T17:48:12ZengMDPI AGPlants2223-77472023-10-011220352110.3390/plants12203521Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild PlantsValentina K. Mursaliyeva0Balaussa T. Sarsenbek1Gulnara T. Dzhakibaeva2Tlek M. Mukhanov3Ramazan Mammadov4Institute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanResearch and Production Center of Microbiology and Virology, Almaty 050010, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty 050040, KazakhstanFaculty of Science, Department of Molecular Biology and Genetics, Mugla University, Mugla 48000, Turkey<i>Allochrusa gypsophiloides</i> is a rare Central Asian species, a super-producer of triterpene saponins with pharmacological and technical value. In this work, a comparative evaluation of the in vitro culture of adventitious roots (ARs), in vitro adventitious microshoots (ASs), natural roots and aboveground parts of wild plants from Kazakhstan to define the total saponin (TS), phenol (TP) and flavonoid (TF) content, as well as antioxidant (AOA) and antimicrobial activity, is presented for the first time. In the AR culture, growth index (GI), TS, TP and TF were evaluated on days 25, 45 and 60 of cultivation on ½ MS medium without (control) and with auxin application. It was found out that TS and TF were higher in the in vitro AR culture. The amount of TP and TF are higher in the aerial part of vegetative plants with maximum AOA. The concentration of the extract required to inhibit 50% of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical formation (ICO<sub>50</sub>) in extracts from natural material negatively correlated with TS, TP, TF and in the in vitro AR culture with TF. Control extracts from the in vitro AR culture with high TS levels showed growth-inhibitory activity against <i>S. thermophillus</i>, <i>S. cerevisiae</i> and <i>C. albicans</i>. The influence shares of medium composition factor, cultivation duration factor and their interaction with GI, TS, TP and TF were determined. The in vitro AR culture is promising for obtaining triterpene saponins TSR with high antibacterial and antifungal activity, and the in vitro ASs culture—for shoot multiplication with antioxidant properties.https://www.mdpi.com/2223-7747/12/20/3521in vitro adventitious roots culture<i>Allochrusa gypsophiloides</i>Turkestan soap rootsaponinsantifungal activity |
| spellingShingle | Valentina K. Mursaliyeva Balaussa T. Sarsenbek Gulnara T. Dzhakibaeva Tlek M. Mukhanov Ramazan Mammadov Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants Plants in vitro adventitious roots culture <i>Allochrusa gypsophiloides</i> Turkestan soap root saponins antifungal activity |
| title | Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants |
| title_full | Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants |
| title_fullStr | Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants |
| title_full_unstemmed | Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants |
| title_short | Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of <i>Allochrusa gypsophiloides</i> (Regel) Schischk Compared to Wild Plants |
| title_sort | total content of saponins phenols and flavonoids and antioxidant and antimicrobial activity of in vitro culture of i allochrusa gypsophiloides i regel schischk compared to wild plants |
| topic | in vitro adventitious roots culture <i>Allochrusa gypsophiloides</i> Turkestan soap root saponins antifungal activity |
| url | https://www.mdpi.com/2223-7747/12/20/3521 |
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