Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep
Forensic genomic systems allow simultaneously analyzing identity informative (iiSNPs), ancestry informative (aiSNPs), and phenotype informative (piSNPs) genetic markers. Among these kits, the ForenSeq DNA Signature prep (Verogen) analyzes identity STRs and SNPs as well as 24 piSNPs from the HIrisPle...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/14/5/1120 |
| _version_ | 1797599960250712064 |
|---|---|
| author | José Alonso Aguilar-Velázquez Blanca Jeannete Llamas-de-Dios Miranda Fabiola Córdova-Mercado Carolina Elena Coronado-Ávila Orlando Salas-Salas Andrés López-Quintero Benito Ramos-González Héctor Rangel-Villalobos |
| author_facet | José Alonso Aguilar-Velázquez Blanca Jeannete Llamas-de-Dios Miranda Fabiola Córdova-Mercado Carolina Elena Coronado-Ávila Orlando Salas-Salas Andrés López-Quintero Benito Ramos-González Héctor Rangel-Villalobos |
| author_sort | José Alonso Aguilar-Velázquez |
| collection | DOAJ |
| description | Forensic genomic systems allow simultaneously analyzing identity informative (iiSNPs), ancestry informative (aiSNPs), and phenotype informative (piSNPs) genetic markers. Among these kits, the ForenSeq DNA Signature prep (Verogen) analyzes identity STRs and SNPs as well as 24 piSNPs from the HIrisPlex system to predict the hair and eye color. We report herein these 24 piSNPs in 88 samples from Monterrey City (Northeast, Mexico) based on the ForenSeq DNA Signature prep. Phenotypes were predicted by genotype results with both Universal Analysis Software (UAS) and the web tool of the Erasmus Medical Center (EMC). We observed predominantly brown eyes (96.5%) and black hair (75%) phenotypes, whereas blue eyes, and blond and red hair were not observed. Both UAS and EMC showed high performance in eye color prediction (<i>p</i> ≥ 96.6%), but a lower accuracy was observed for hair color prediction. Overall, UAS hair color predictions showed better performance and robustness than those obtained with the EMC web tool (when hair shade is excluded). Although we employed a threshold (<i>p</i> > 70%), we suggest using the EMC enhanced approach to avoid the exclusion of a high number of samples. Finally, although our results are helpful to employ these genomic tools to predict eye color, caution is suggested for hair color prediction in Latin American (admixed) populations such as those studied herein, principally when no black color is predicted. |
| first_indexed | 2024-03-11T03:41:43Z |
| format | Article |
| id | doaj.art-961e233f31734b3e9ca521d3bbf39367 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T03:41:43Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-961e233f31734b3e9ca521d3bbf393672023-11-18T01:30:44ZengMDPI AGGenes2073-44252023-05-01145112010.3390/genes14051120Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature PrepJosé Alonso Aguilar-Velázquez0Blanca Jeannete Llamas-de-Dios1Miranda Fabiola Córdova-Mercado2Carolina Elena Coronado-Ávila3Orlando Salas-Salas4Andrés López-Quintero5Benito Ramos-González6Héctor Rangel-Villalobos7Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara 44340, Jalisco, MexicoLicenciatura en Ciencias Forenses, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara 44340, Jalisco, MexicoInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey 64720, Nuevo León, MexicoInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey 64720, Nuevo León, MexicoInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey 64720, Nuevo León, MexicoInstituto de Nutrigenética y Nutrigenómica Traslacional, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara 44340, Jalisco, MexicoInstituto de Criminalística y Servicios Periciales, Fiscalía General de Justicia del Estado de Nuevo León (FGJNL), Monterrey 64720, Nuevo León, MexicoInstituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCI-UdeG), Ocotlán 47820, Jalisco, MexicoForensic genomic systems allow simultaneously analyzing identity informative (iiSNPs), ancestry informative (aiSNPs), and phenotype informative (piSNPs) genetic markers. Among these kits, the ForenSeq DNA Signature prep (Verogen) analyzes identity STRs and SNPs as well as 24 piSNPs from the HIrisPlex system to predict the hair and eye color. We report herein these 24 piSNPs in 88 samples from Monterrey City (Northeast, Mexico) based on the ForenSeq DNA Signature prep. Phenotypes were predicted by genotype results with both Universal Analysis Software (UAS) and the web tool of the Erasmus Medical Center (EMC). We observed predominantly brown eyes (96.5%) and black hair (75%) phenotypes, whereas blue eyes, and blond and red hair were not observed. Both UAS and EMC showed high performance in eye color prediction (<i>p</i> ≥ 96.6%), but a lower accuracy was observed for hair color prediction. Overall, UAS hair color predictions showed better performance and robustness than those obtained with the EMC web tool (when hair shade is excluded). Although we employed a threshold (<i>p</i> > 70%), we suggest using the EMC enhanced approach to avoid the exclusion of a high number of samples. Finally, although our results are helpful to employ these genomic tools to predict eye color, caution is suggested for hair color prediction in Latin American (admixed) populations such as those studied herein, principally when no black color is predicted.https://www.mdpi.com/2073-4425/14/5/1120MPSForenseqphenotypesMexicogenomicspiSNPs |
| spellingShingle | José Alonso Aguilar-Velázquez Blanca Jeannete Llamas-de-Dios Miranda Fabiola Córdova-Mercado Carolina Elena Coronado-Ávila Orlando Salas-Salas Andrés López-Quintero Benito Ramos-González Héctor Rangel-Villalobos Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep Genes MPS Forenseq phenotypes Mexico genomics piSNPs |
| title | Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep |
| title_full | Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep |
| title_fullStr | Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep |
| title_full_unstemmed | Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep |
| title_short | Accuracy of Eye and Hair Color Prediction in Mexican Mestizos from Monterrey City Based on ForenSeq<sup>TM</sup> DNA Signature Prep |
| title_sort | accuracy of eye and hair color prediction in mexican mestizos from monterrey city based on forenseq sup tm sup dna signature prep |
| topic | MPS Forenseq phenotypes Mexico genomics piSNPs |
| url | https://www.mdpi.com/2073-4425/14/5/1120 |
| work_keys_str_mv | AT josealonsoaguilarvelazquez accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT blancajeannetellamasdedios accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT mirandafabiolacordovamercado accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT carolinaelenacoronadoavila accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT orlandosalassalas accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT andreslopezquintero accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT benitoramosgonzalez accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep AT hectorrangelvillalobos accuracyofeyeandhaircolorpredictioninmexicanmestizosfrommonterreycitybasedonforenseqsuptmsupdnasignatureprep |