Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model
Abstract Background Local ischemic preconditioning (IPC) and remote ischemic conditioning (RIC) induced by brief periods of ischemia and reperfusion protect against ischemia‐reperfusion injury. Methods We studied the sensitivity to IR‐injury and the influence of strain, age, supplier, and anesthesia...
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| Format: | Article |
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Wiley
2021-04-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14810 |
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| author | Thomas Ravn Lassen Marie Vognstoft Hjortbak Marie Hauerslev Pernille Tilma Tonnesen Steen Buus Kristiansen Rebekka Vibjerg Jensen Hans Erik Bøtker |
| author_facet | Thomas Ravn Lassen Marie Vognstoft Hjortbak Marie Hauerslev Pernille Tilma Tonnesen Steen Buus Kristiansen Rebekka Vibjerg Jensen Hans Erik Bøtker |
| author_sort | Thomas Ravn Lassen |
| collection | DOAJ |
| description | Abstract Background Local ischemic preconditioning (IPC) and remote ischemic conditioning (RIC) induced by brief periods of ischemia and reperfusion protect against ischemia‐reperfusion injury. Methods We studied the sensitivity to IR‐injury and the influence of strain, age, supplier, and anesthesia upon the efficacy of IPC and RIC in 7‐ and 16‐weeks‐old Sprague‐Dawley and Wistar rats from three different suppliers. The influence of sedation with a hypnorm and midazolam mixture (rodent mixture) and pentobarbiturate was compared. Results IPC attenuated infarct size in both 7‐weeks‐old Sprague–Dawley (48.4 ± 17.7% vs. 20.3 ± 6.9, p < 0.001) and 7‐weeks‐old Wistar (55.6 ± 10.9% vs. 26.8 ± 5.0%, p < 0.001) rats. Infarct size was larger in 16‐weeks‐old Sprague–Dawley rats, however, IPC still lowered infarct size (78.8 ± 9.2% vs. 58.3 ± 12.3%, p < 0.01). RIC reduced infarct sizes in 7‐weeks‐old Sprague–Dawley (75.3 ± 11.8% vs. 58.6 ± 8.9%, p < 0.05), but not in 7‐weeks‐old Wistar rats (31.7 ± 17.6% and 24.0 ± 12.6%, p = 0.2). In 16‐weeks‐old Sprague–Dawley rats, RIC did not induce protection (76.4 ± 5.5% and 73.2 ± 14.7%, p = 0.6). However, RIC induced protection in 16‐weeks‐old Wistar rats (45.2 ± 8.5% vs. 14.7 ± 10.8%, p < 0.001). RIC did not reduce infarct size in 7‐weeks‐old Sprague–Dawley rats from Charles River (62.0 ± 13.5% and 69.4 ± 10.4% p = 0.3) or 16‐weeks‐old Wistar rats from Janvier (50.7 ± 11.3 and 49.2 ± 16.2, p = 0.8). There was no difference between sedation with rodent mixture or pentobarbiturate. Conclusion The cardioprotective effect of IPC is consistent across rat strains independent of age, strain, and supplier. RIC seems to be less reproducible, but still yields protection across different rat strains. However, age, animal supplier, and anesthetics may modulate the sensitivity of IR‐injury and the response to RIC. |
| first_indexed | 2024-12-20T05:42:28Z |
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| id | doaj.art-961e68534d674ea8a4e93dcf6c49230d |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-20T05:42:28Z |
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| spelling | doaj.art-961e68534d674ea8a4e93dcf6c49230d2022-12-21T19:51:25ZengWileyPhysiological Reports2051-817X2021-04-0197n/an/a10.14814/phy2.14810Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat modelThomas Ravn Lassen0Marie Vognstoft Hjortbak1Marie Hauerslev2Pernille Tilma Tonnesen3Steen Buus Kristiansen4Rebekka Vibjerg Jensen5Hans Erik Bøtker6Department of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkDepartment of Cardiology Aarhus University Hospital Aarhus N DenmarkAbstract Background Local ischemic preconditioning (IPC) and remote ischemic conditioning (RIC) induced by brief periods of ischemia and reperfusion protect against ischemia‐reperfusion injury. Methods We studied the sensitivity to IR‐injury and the influence of strain, age, supplier, and anesthesia upon the efficacy of IPC and RIC in 7‐ and 16‐weeks‐old Sprague‐Dawley and Wistar rats from three different suppliers. The influence of sedation with a hypnorm and midazolam mixture (rodent mixture) and pentobarbiturate was compared. Results IPC attenuated infarct size in both 7‐weeks‐old Sprague–Dawley (48.4 ± 17.7% vs. 20.3 ± 6.9, p < 0.001) and 7‐weeks‐old Wistar (55.6 ± 10.9% vs. 26.8 ± 5.0%, p < 0.001) rats. Infarct size was larger in 16‐weeks‐old Sprague–Dawley rats, however, IPC still lowered infarct size (78.8 ± 9.2% vs. 58.3 ± 12.3%, p < 0.01). RIC reduced infarct sizes in 7‐weeks‐old Sprague–Dawley (75.3 ± 11.8% vs. 58.6 ± 8.9%, p < 0.05), but not in 7‐weeks‐old Wistar rats (31.7 ± 17.6% and 24.0 ± 12.6%, p = 0.2). In 16‐weeks‐old Sprague–Dawley rats, RIC did not induce protection (76.4 ± 5.5% and 73.2 ± 14.7%, p = 0.6). However, RIC induced protection in 16‐weeks‐old Wistar rats (45.2 ± 8.5% vs. 14.7 ± 10.8%, p < 0.001). RIC did not reduce infarct size in 7‐weeks‐old Sprague–Dawley rats from Charles River (62.0 ± 13.5% and 69.4 ± 10.4% p = 0.3) or 16‐weeks‐old Wistar rats from Janvier (50.7 ± 11.3 and 49.2 ± 16.2, p = 0.8). There was no difference between sedation with rodent mixture or pentobarbiturate. Conclusion The cardioprotective effect of IPC is consistent across rat strains independent of age, strain, and supplier. RIC seems to be less reproducible, but still yields protection across different rat strains. However, age, animal supplier, and anesthetics may modulate the sensitivity of IR‐injury and the response to RIC.https://doi.org/10.14814/phy2.14810cardioprotectionheartischemic conditioningisolated heart preparation |
| spellingShingle | Thomas Ravn Lassen Marie Vognstoft Hjortbak Marie Hauerslev Pernille Tilma Tonnesen Steen Buus Kristiansen Rebekka Vibjerg Jensen Hans Erik Bøtker Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model Physiological Reports cardioprotection heart ischemic conditioning isolated heart preparation |
| title | Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| title_full | Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| title_fullStr | Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| title_full_unstemmed | Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| title_short | Influence of strain, age, origin, and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| title_sort | influence of strain age origin and anesthesia on the cardioprotective efficacy by local and remote ischemic conditioning in an ex vivo rat model |
| topic | cardioprotection heart ischemic conditioning isolated heart preparation |
| url | https://doi.org/10.14814/phy2.14810 |
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