Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA
AbstractAs the main pathological basis for the development of cardiovascular and cerebrovascular diseases, atherosclerosis (AS) seriously affects human health. The key targets of biological information analysis of AS can help exploit therapeutic targets. The expression data of early and progressive...
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| Format: | Article |
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Taylor & Francis Group
2023-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2023.2203185 |
| _version_ | 1797631207226212352 |
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| author | Jing Wen Tong Ren Jinzhen Zheng Xing Jiang Yingxia Li Xilin Jiang Xin Jin Hongying Zhao Jingwen Li |
| author_facet | Jing Wen Tong Ren Jinzhen Zheng Xing Jiang Yingxia Li Xilin Jiang Xin Jin Hongying Zhao Jingwen Li |
| author_sort | Jing Wen |
| collection | DOAJ |
| description | AbstractAs the main pathological basis for the development of cardiovascular and cerebrovascular diseases, atherosclerosis (AS) seriously affects human health. The key targets of biological information analysis of AS can help exploit therapeutic targets. The expression data of early and progressive atherosclerotic tissues were downloaded from the Gene Expression Omnibus (GEO) database. Based on GSE28829 and GSE120521, 74 key genes were obtained through differential expression analysis and weighted correlation network analysis (WGCNA) analysis, which were mainly enriched in the regulating of inflammatory response, chemokine signalling pathway, apoptosis, lipid and AS, Toll-like receptor signalling pathway and so on according to the results of the enrichment analysis. Cytoscape software was applied to screen four pivotal genes (TYROBP, ITGB2, ITGAM and TLR2) based on PPI. The results of the correlation analysis showed that the expression level of pivotal genes was positively related to macrophages M0, and was negatively related to T cells follicular helper. In addition, the expression of ITGB2 was positively related to Tregs. In this study, bioinformatics was applied to screen pivotal genes affecting the progress of AS, which were significantly related to immune-related biological functions and signal pathways of atherosclerotic tissues and the infiltration level of immune cells. Therefore, pivotal genes were expected to become therapeutic targets for AS. |
| first_indexed | 2024-03-11T11:17:38Z |
| format | Article |
| id | doaj.art-961e725c7510487d8eb558e9ba5a8645 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-03-11T11:17:38Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-961e725c7510487d8eb558e9ba5a86452023-11-10T17:19:33ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2023-12-0151127628510.1080/21691401.2023.2203185Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNAJing Wen0Tong Ren1Jinzhen Zheng2Xing Jiang3Yingxia Li4Xilin Jiang5Xin Jin6Hongying Zhao7Jingwen Li8Xiamen Cardiovascular Hospital of Xiamen University, School of Medcine, Xiamen University, Xiamen, ChinaXiamen Cardiovascular Hospital of Xiamen University, School of Medcine, Xiamen University, Xiamen, ChinaXiamen Cardiovascular Hospital of Xiamen University, School of Medcine, Xiamen University, Xiamen, ChinaDepartment of Neurosurgery, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaDepartment of Endocrinology, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Cardiovascular Hospital of Xiamen University, School of Medcine, Xiamen University, Xiamen, ChinaXiamen Cardiovascular Hospital of Xiamen University, School of Medcine, Xiamen University, Xiamen, ChinaDepartment of Pediatrics, Zhongshan Hospital of Xiamen University, Xiamen University, Xiamen, ChinaDepartment of Neurosurgery, Xiang’an Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaAbstractAs the main pathological basis for the development of cardiovascular and cerebrovascular diseases, atherosclerosis (AS) seriously affects human health. The key targets of biological information analysis of AS can help exploit therapeutic targets. The expression data of early and progressive atherosclerotic tissues were downloaded from the Gene Expression Omnibus (GEO) database. Based on GSE28829 and GSE120521, 74 key genes were obtained through differential expression analysis and weighted correlation network analysis (WGCNA) analysis, which were mainly enriched in the regulating of inflammatory response, chemokine signalling pathway, apoptosis, lipid and AS, Toll-like receptor signalling pathway and so on according to the results of the enrichment analysis. Cytoscape software was applied to screen four pivotal genes (TYROBP, ITGB2, ITGAM and TLR2) based on PPI. The results of the correlation analysis showed that the expression level of pivotal genes was positively related to macrophages M0, and was negatively related to T cells follicular helper. In addition, the expression of ITGB2 was positively related to Tregs. In this study, bioinformatics was applied to screen pivotal genes affecting the progress of AS, which were significantly related to immune-related biological functions and signal pathways of atherosclerotic tissues and the infiltration level of immune cells. Therefore, pivotal genes were expected to become therapeutic targets for AS.https://www.tandfonline.com/doi/10.1080/21691401.2023.2203185Atherosclerosisweighted correlation network analysisgene set enrichment analysisimmune cell |
| spellingShingle | Jing Wen Tong Ren Jinzhen Zheng Xing Jiang Yingxia Li Xilin Jiang Xin Jin Hongying Zhao Jingwen Li Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA Artificial Cells, Nanomedicine, and Biotechnology Atherosclerosis weighted correlation network analysis gene set enrichment analysis immune cell |
| title | Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA |
| title_full | Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA |
| title_fullStr | Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA |
| title_full_unstemmed | Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA |
| title_short | Identification and verification of pivotal genes promoting the progression of atherosclerosis based on WGCNA |
| title_sort | identification and verification of pivotal genes promoting the progression of atherosclerosis based on wgcna |
| topic | Atherosclerosis weighted correlation network analysis gene set enrichment analysis immune cell |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2023.2203185 |
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