Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk
Peptides, but not whole protein, elicit an allergic reaction since food allergens should be consumed by digestion. In this study, we explored the remaining peptides after simulated digestion of cow’s milk in order to search for β-lactoglobulin (BLG)-derived peptides that could play an immunomodulato...
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MDPI AG
2022-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/10/1450 |
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| author | Xin Ma Fan Yang Xuanyi Meng Yong Wu Ping Tong Jinyan Gao Hongbing Chen Xin Li |
| author_facet | Xin Ma Fan Yang Xuanyi Meng Yong Wu Ping Tong Jinyan Gao Hongbing Chen Xin Li |
| author_sort | Xin Ma |
| collection | DOAJ |
| description | Peptides, but not whole protein, elicit an allergic reaction since food allergens should be consumed by digestion. In this study, we explored the remaining peptides after simulated digestion of cow’s milk in order to search for β-lactoglobulin (BLG)-derived peptides that could play an immunomodulatory role. As a major allergen in milk, BLG-derived peptides, 109 in total, were identified both from simulated infant and adult digestion in vitro. These peptides were mainly located in four regions, and they were synthesized as five peptides, namely, BLG<sub>1–14</sub>, BLG<sub>24–35</sub>, BLG<sub>40–60</sub>, BLG<sub>82–101</sub>, and BLG<sub>123–139</sub>. Then, the effect of peptides on the Caco-2 cell’s transport absorption, the co-stimulatory molecules of DC, and the T-cell phenotype was explored. The results suggested all peptides showed better transport absorption capacity with the apparent permeability coefficient higher than 2 × 10<sup>−6</sup> cm·s<sup>−1</sup>. The ability of BLG<sub>40–60</sub> for promoting lamina propria-derived DC cell (LPDC) maturation was observed by the increase in MHC II. Moreover, BLG<sub>1–14</sub> and BLG<sub>40–60</sub> directed activation of T lymphocytes towards a Th1 phenotype. This is the first report of the immunomodulatory potential of peptides in the sensitization of allergic reaction, and one peptide, BLG<sub>40–60</sub>, was regarded as an immunomodulatory peptide, one that should be further explored in an animal model in depth. |
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| language | English |
| last_indexed | 2024-03-10T03:54:18Z |
| publishDate | 2022-05-01 |
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| series | Foods |
| spelling | doaj.art-9620b7511a184ec5a96f1f20b190b0a12023-11-23T10:59:19ZengMDPI AGFoods2304-81582022-05-011110145010.3390/foods11101450Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s MilkXin Ma0Fan Yang1Xuanyi Meng2Yong Wu3Ping Tong4Jinyan Gao5Hongbing Chen6Xin Li7State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaPeptides, but not whole protein, elicit an allergic reaction since food allergens should be consumed by digestion. In this study, we explored the remaining peptides after simulated digestion of cow’s milk in order to search for β-lactoglobulin (BLG)-derived peptides that could play an immunomodulatory role. As a major allergen in milk, BLG-derived peptides, 109 in total, were identified both from simulated infant and adult digestion in vitro. These peptides were mainly located in four regions, and they were synthesized as five peptides, namely, BLG<sub>1–14</sub>, BLG<sub>24–35</sub>, BLG<sub>40–60</sub>, BLG<sub>82–101</sub>, and BLG<sub>123–139</sub>. Then, the effect of peptides on the Caco-2 cell’s transport absorption, the co-stimulatory molecules of DC, and the T-cell phenotype was explored. The results suggested all peptides showed better transport absorption capacity with the apparent permeability coefficient higher than 2 × 10<sup>−6</sup> cm·s<sup>−1</sup>. The ability of BLG<sub>40–60</sub> for promoting lamina propria-derived DC cell (LPDC) maturation was observed by the increase in MHC II. Moreover, BLG<sub>1–14</sub> and BLG<sub>40–60</sub> directed activation of T lymphocytes towards a Th1 phenotype. This is the first report of the immunomodulatory potential of peptides in the sensitization of allergic reaction, and one peptide, BLG<sub>40–60</sub>, was regarded as an immunomodulatory peptide, one that should be further explored in an animal model in depth.https://www.mdpi.com/2304-8158/11/10/1450cow’s milkdigested peptidesunique peptidesimmunologic function |
| spellingShingle | Xin Ma Fan Yang Xuanyi Meng Yong Wu Ping Tong Jinyan Gao Hongbing Chen Xin Li Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk Foods cow’s milk digested peptides unique peptides immunologic function |
| title | Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk |
| title_full | Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk |
| title_fullStr | Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk |
| title_full_unstemmed | Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk |
| title_short | Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow’s Milk |
| title_sort | immunomodulatory role of blg derived peptides based on simulated gastrointestinal digestion and dc t cell from mice allergic to cow s milk |
| topic | cow’s milk digested peptides unique peptides immunologic function |
| url | https://www.mdpi.com/2304-8158/11/10/1450 |
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