OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice
Abstract Background Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2021-05-01
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| Series: | Rice |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12284-021-00485-w |
| _version_ | 1818640006797852672 |
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| author | Hongjia Zhang Tao Wu Zhao Li Kai Huang Na-Eun Kim Ziming Ma Soon-Wook Kwon Wenzhu Jiang Xinglin Du |
| author_facet | Hongjia Zhang Tao Wu Zhao Li Kai Huang Na-Eun Kim Ziming Ma Soon-Wook Kwon Wenzhu Jiang Xinglin Du |
| author_sort | Hongjia Zhang |
| collection | DOAJ |
| description | Abstract Background Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear. Results In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45–1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45–1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336G) associated with cold tolerance. Conclusion OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45–1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336G) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding. |
| first_indexed | 2024-12-16T23:04:25Z |
| format | Article |
| id | doaj.art-96219aed24474e388a138e6730d37651 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-16T23:04:25Z |
| publishDate | 2021-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-96219aed24474e388a138e6730d376512022-12-21T22:12:36ZengSpringerOpenRice1939-84251939-84332021-05-0114111510.1186/s12284-021-00485-wOsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in RiceHongjia Zhang0Tao Wu1Zhao Li2Kai Huang3Na-Eun Kim4Ziming Ma5Soon-Wook Kwon6Wenzhu Jiang7Xinglin Du8Jilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityDepartment of Plant Bioscience, College of Natural Resources and Life Science, Pusan National UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityDepartment of Plant Bioscience, College of Natural Resources and Life Science, Pusan National UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityJilin Province Engineering Laboratory of Plant Genetic Improvement, College of Plant Science, Jilin UniversityAbstract Background Cold stress is the main abiotic stress in rice, which seriously affects the growth and yield of rice. Identification of cold tolerance genes is of great significance for rice to solve these problems. GATA-family transcription factors involve diverse biological functions, however, their role in cold tolerance in rice remains unclear. Results In this study, a GATA-type zinc finger transcription factor OsGATA16, which can improve cold tolerance, was isolated and characterized from rice. OsGATA16 belongs to OsGATA subfamily-II and contains 11 putative phosphorylation sites, a nuclear localization signal (NLS), and other several conserved domains. OsGATA16 was expressed in all plant tissues, with the strongest in panicles. It was induced by cold and ABA treatments, but was repressed by drought, cytokinin and JA, and acted as a transcriptional suppressor in the nucleus. Overexpression of OsGATA16 improves cold tolerance of rice at seedling stage. Under cold stress treatments, the transcription of four cold-related genes OsWRKY45–1, OsSRFP1, OsCYL4, and OsMYB30 was repressed in OsGATA16-overexpressing (OE) rice compared with wild-type (WT). Interestingly, OsGATA16 bound to the promoter of OsWRKY45–1 and repressed its expression. In addition, haplotype analysis showed that OsGATA16 polarized between the two major rice subspecies japonica and indica, and had a non-synonymous SNP8 (336G) associated with cold tolerance. Conclusion OsGATA16 is a GATA transcription factor, which improves cold tolerance at seedling stage in rice. It acts as a positive regulator of cold tolerance by repressing some cold-related genes such as OsWRKY45–1, OsSRFP1, OsCYL4 and OsMYB30. Additionally, OsGATA16 has a non-synonymous SNP8 (336G) associated with cold tolerance on CDS region. This study provides a theoretical basis for elucidating the mechanism of cold tolerance in rice and new germplasm resources for rice breeding.https://doi.org/10.1186/s12284-021-00485-wRiceTranscription factorOsGATA16HaplotypeCold tolerance |
| spellingShingle | Hongjia Zhang Tao Wu Zhao Li Kai Huang Na-Eun Kim Ziming Ma Soon-Wook Kwon Wenzhu Jiang Xinglin Du OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice Rice Rice Transcription factor OsGATA16 Haplotype Cold tolerance |
| title | OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice |
| title_full | OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice |
| title_fullStr | OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice |
| title_full_unstemmed | OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice |
| title_short | OsGATA16, a GATA Transcription Factor, Confers Cold Tolerance by Repressing OsWRKY45–1 at the Seedling Stage in Rice |
| title_sort | osgata16 a gata transcription factor confers cold tolerance by repressing oswrky45 1 at the seedling stage in rice |
| topic | Rice Transcription factor OsGATA16 Haplotype Cold tolerance |
| url | https://doi.org/10.1186/s12284-021-00485-w |
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