Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment
<p>Abstract</p> <p>Background</p> <p>Lignin is embedded in the plant cell wall matrix, and impedes the enzymatic saccharification of lignocellulosic feedstocks. To investigate whether enzymatic digestibility of cell wall materials can be improved by altering the relativ...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-12-01
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| Series: | Biotechnology for Biofuels |
| Online Access: | http://www.biotechnologyforbiofuels.com/content/3/1/27 |
| _version_ | 1811340490243571712 |
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| author | Ladisch Michael Meilan Richard Slininger Mary Kim Youngmi Ximenes Eduardo Li Xu Chapple Clint |
| author_facet | Ladisch Michael Meilan Richard Slininger Mary Kim Youngmi Ximenes Eduardo Li Xu Chapple Clint |
| author_sort | Ladisch Michael |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Lignin is embedded in the plant cell wall matrix, and impedes the enzymatic saccharification of lignocellulosic feedstocks. To investigate whether enzymatic digestibility of cell wall materials can be improved by altering the relative abundance of the two major lignin monomers, guaiacyl (G) and syringyl (S) subunits, we compared the degradability of cell wall material from wild-type <it>Arabidopsis thaliana </it>with a mutant line and a genetically modified line, the lignins of which are enriched in G and S subunits, respectively.</p> <p>Results</p> <p>Arabidopsis tissue containing G- and S-rich lignins had the same saccharification performance as the wild type when subjected to enzyme hydrolysis without pretreatment. After a 24-hour incubation period, less than 30% of the total glucan was hydrolyzed. By contrast, when liquid hot water (LHW) pretreatment was included before enzyme hydrolysis, the S-lignin-rich tissue gave a much higher glucose yield than either the wild-type or G-lignin-rich tissue. Applying a hot-water washing step after the pretreatment did not lead to a further increase in final glucose yield, but the initial hydrolytic rate was doubled.</p> <p>Conclusions</p> <p>Our analyses using the model plant <it>A. thaliana </it>revealed that lignin composition affects the enzymatic digestibility of LHW pretreated plant material. Pretreatment is more effective in enhancing the saccharification of <it>A. thaliana </it>cell walls that contain S-rich lignin. Increasing lignin S monomer content through genetic engineering may be a promising approach to increase the efficiency and reduce the cost of biomass to biofuel conversion.</p> |
| first_indexed | 2024-04-13T18:42:44Z |
| format | Article |
| id | doaj.art-9622fb7d25924983900744da5010adf6 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T18:42:44Z |
| publishDate | 2010-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-9622fb7d25924983900744da5010adf62022-12-22T02:34:41ZengBMCBiotechnology for Biofuels1754-68342010-12-01312710.1186/1754-6834-3-27Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatmentLadisch MichaelMeilan RichardSlininger MaryKim YoungmiXimenes EduardoLi XuChapple Clint<p>Abstract</p> <p>Background</p> <p>Lignin is embedded in the plant cell wall matrix, and impedes the enzymatic saccharification of lignocellulosic feedstocks. To investigate whether enzymatic digestibility of cell wall materials can be improved by altering the relative abundance of the two major lignin monomers, guaiacyl (G) and syringyl (S) subunits, we compared the degradability of cell wall material from wild-type <it>Arabidopsis thaliana </it>with a mutant line and a genetically modified line, the lignins of which are enriched in G and S subunits, respectively.</p> <p>Results</p> <p>Arabidopsis tissue containing G- and S-rich lignins had the same saccharification performance as the wild type when subjected to enzyme hydrolysis without pretreatment. After a 24-hour incubation period, less than 30% of the total glucan was hydrolyzed. By contrast, when liquid hot water (LHW) pretreatment was included before enzyme hydrolysis, the S-lignin-rich tissue gave a much higher glucose yield than either the wild-type or G-lignin-rich tissue. Applying a hot-water washing step after the pretreatment did not lead to a further increase in final glucose yield, but the initial hydrolytic rate was doubled.</p> <p>Conclusions</p> <p>Our analyses using the model plant <it>A. thaliana </it>revealed that lignin composition affects the enzymatic digestibility of LHW pretreated plant material. Pretreatment is more effective in enhancing the saccharification of <it>A. thaliana </it>cell walls that contain S-rich lignin. Increasing lignin S monomer content through genetic engineering may be a promising approach to increase the efficiency and reduce the cost of biomass to biofuel conversion.</p>http://www.biotechnologyforbiofuels.com/content/3/1/27 |
| spellingShingle | Ladisch Michael Meilan Richard Slininger Mary Kim Youngmi Ximenes Eduardo Li Xu Chapple Clint Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment Biotechnology for Biofuels |
| title | Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment |
| title_full | Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment |
| title_fullStr | Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment |
| title_full_unstemmed | Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment |
| title_short | Lignin monomer composition affects <it>Arabidopsis </it>cell-wall degradability after liquid hot water pretreatment |
| title_sort | lignin monomer composition affects it arabidopsis it cell wall degradability after liquid hot water pretreatment |
| url | http://www.biotechnologyforbiofuels.com/content/3/1/27 |
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