Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples
New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan <i>Leishmania.</i> Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of <i>Leishmania</i> spp. in animal tissues, yet it wa...
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MDPI AG
2022-11-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/7/11/344 |
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| author | Luiz Cláudio Ferreira Leonardo Pereira Quintella Armando de Oliveira Schubach Luciana de Freitas Campos Miranda Maria de Fátima Madeira Maria Inês Fernandes Pimentel Érica de Camargo Ferreira e Vasconcellos Marcelo Rosandiski Lyra Raquel de Vasconcellos Carvalhaes de Oliveira Rodrigo Caldas Menezes |
| author_facet | Luiz Cláudio Ferreira Leonardo Pereira Quintella Armando de Oliveira Schubach Luciana de Freitas Campos Miranda Maria de Fátima Madeira Maria Inês Fernandes Pimentel Érica de Camargo Ferreira e Vasconcellos Marcelo Rosandiski Lyra Raquel de Vasconcellos Carvalhaes de Oliveira Rodrigo Caldas Menezes |
| author_sort | Luiz Cláudio Ferreira |
| collection | DOAJ |
| description | New world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan <i>Leishmania.</i> Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of <i>Leishmania</i> spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by <i>L. (V.) braziliensis</i>. These specimens were analyzed by CISH, using a generic probe for <i>Leishmania</i>, IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by <i>L.</i> (<i>V</i>.) <i>braziliensis</i>. |
| first_indexed | 2024-03-09T18:35:23Z |
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| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-09T18:35:23Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-9623be092f244d2890c2d7c3582e430c2023-11-24T07:11:25ZengMDPI AGTropical Medicine and Infectious Disease2414-63662022-11-0171134410.3390/tropicalmed7110344Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin SamplesLuiz Cláudio Ferreira0Leonardo Pereira Quintella1Armando de Oliveira Schubach2Luciana de Freitas Campos Miranda3Maria de Fátima Madeira4Maria Inês Fernandes Pimentel5Érica de Camargo Ferreira e Vasconcellos6Marcelo Rosandiski Lyra7Raquel de Vasconcellos Carvalhaes de Oliveira8Rodrigo Caldas Menezes9Anatomic Pathology Service, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilAnatomic Pathology Service, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilFundação Técnico Educacional Souza Marques, Av. Ernani Cardoso, 335, Rio de Janeiro 21310-310, BrazilLaboratory of Clinical Research and Surveillance of Leishmaniasis, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Epidemiology, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilLaboratory of Clinical Research on Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, Oswaldo Cruz Foundation, Av. Brasil, 4365, Rio de Janeiro 21040-360, BrazilNew world cutaneous leishmaniasis (NWCL) is an anthropozoonosis caused by different species of the protozoan <i>Leishmania.</i> Colorimetric in situ hybridization (CISH) was shown to satisfactorily detect amastigote forms of <i>Leishmania</i> spp. in animal tissues, yet it was not tested for the diagnosis of human NWCL. The aim of this study was to compare CISH, histopathology (HP), and immunohistochemistry (IHC) techniques to diagnose NWCL in human cutaneous lesions. The sample comprised fifty formalin-fixed, paraffin-embedded skin biopsy specimens from patients with NWCL caused by <i>L. (V.) braziliensis</i>. These specimens were analyzed by CISH, using a generic probe for <i>Leishmania</i>, IHC, and HP to assess the sensitivity of these methods by using a parasitological culture as a standard reference. Additional specimens from three patients diagnosed with cutaneous mycoses were also included to evaluate cross-reactions between CISH and IHC. The sensitivities of IHC, CISH, and HP for detecting amastigotes was 66%, 54%, and 50%, respectively. IHC, unlike CISH, cross-reacted with different species of fungi. Together, these results demonstrate that CISH may be a complementary assay for the detection of amastigote in the laboratorial diagnosis routine of human NWCL caused by <i>L.</i> (<i>V</i>.) <i>braziliensis</i>.https://www.mdpi.com/2414-6366/7/11/344tegumentary leishmaniasisdiagnosis<i>Leishmania braziliensis</i>in situ hybridizationimmunoperoxidase |
| spellingShingle | Luiz Cláudio Ferreira Leonardo Pereira Quintella Armando de Oliveira Schubach Luciana de Freitas Campos Miranda Maria de Fátima Madeira Maria Inês Fernandes Pimentel Érica de Camargo Ferreira e Vasconcellos Marcelo Rosandiski Lyra Raquel de Vasconcellos Carvalhaes de Oliveira Rodrigo Caldas Menezes Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples Tropical Medicine and Infectious Disease tegumentary leishmaniasis diagnosis <i>Leishmania braziliensis</i> in situ hybridization immunoperoxidase |
| title | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples |
| title_full | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples |
| title_fullStr | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples |
| title_full_unstemmed | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples |
| title_short | Comparison between Colorimetric In Situ Hybridization, Histopathology, and Immunohistochemistry for the Diagnosis of New World Cutaneous Leishmaniasis in Human Skin Samples |
| title_sort | comparison between colorimetric in situ hybridization histopathology and immunohistochemistry for the diagnosis of new world cutaneous leishmaniasis in human skin samples |
| topic | tegumentary leishmaniasis diagnosis <i>Leishmania braziliensis</i> in situ hybridization immunoperoxidase |
| url | https://www.mdpi.com/2414-6366/7/11/344 |
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