Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)
A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2007-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640702119M.pdf |
| _version_ | 1811247515795718144 |
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| author | Milisavljević Mira Đ. Timotijević Gordana S. Radović Svetlana R. Konstantinović M.M. Maksimović Vesna R. |
| author_facet | Milisavljević Mira Đ. Timotijević Gordana S. Radović Svetlana R. Konstantinović M.M. Maksimović Vesna R. |
| author_sort | Milisavljević Mira Đ. |
| collection | DOAJ |
| description | A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genome showed that most potential AP genes are intronless and PSI-less, it appears that "atypical" is an inappropriate word for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants. . |
| first_indexed | 2024-04-12T15:11:09Z |
| format | Article |
| id | doaj.art-96243575edc345b782e4f5b647a17b14 |
| institution | Directory Open Access Journal |
| issn | 0354-4664 |
| language | English |
| last_indexed | 2024-04-12T15:11:09Z |
| publishDate | 2007-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-96243575edc345b782e4f5b647a17b142022-12-22T03:27:46ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642007-01-0159211912410.2298/ABS0702119MIsolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)Milisavljević Mira Đ.Timotijević Gordana S.Radović Svetlana R.Konstantinović M.M.Maksimović Vesna R.A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genome showed that most potential AP genes are intronless and PSI-less, it appears that "atypical" is an inappropriate word for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants. .http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640702119M.pdfaspartic proteinasebuckwheatcDNA |
| spellingShingle | Milisavljević Mira Đ. Timotijević Gordana S. Radović Svetlana R. Konstantinović M.M. Maksimović Vesna R. Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) Archives of Biological Sciences aspartic proteinase buckwheat cDNA |
| title | Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) |
| title_full | Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) |
| title_fullStr | Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) |
| title_full_unstemmed | Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) |
| title_short | Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench) |
| title_sort | isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed fagopyrum esculentum moench |
| topic | aspartic proteinase buckwheat cDNA |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2007/0354-46640702119M.pdf |
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