Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces
We explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedd...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/9/4/786 |
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| author | Irina Negut Anita Ioana Visan Camelia Popescu Rodica Cristescu Anton Ficai Alexandru Mihai Grumezescu Mariana C. Chifiriuc Ryan D. Boehm Dina Yamaleyeva Michael Taylor Roger J. Narayan Douglas B. Chrisey |
| author_facet | Irina Negut Anita Ioana Visan Camelia Popescu Rodica Cristescu Anton Ficai Alexandru Mihai Grumezescu Mariana C. Chifiriuc Ryan D. Boehm Dina Yamaleyeva Michael Taylor Roger J. Narayan Douglas B. Chrisey |
| author_sort | Irina Negut |
| collection | DOAJ |
| description | We explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedded in a polyvinylpyrrolidone biopolymer; the antifungal activity of the film components was evaluated using <i>in vitro</i> microbiological assays. Thin films were deposited using a pulsed KrF* excimer laser source which were structurally characterized using atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). High-quality thin films with chemical structures similar to dropcast ones were created using an optimum laser fluence of ~80 mJ/cm<sup>2</sup>. Bioactive substances were included within the polymer thin films using the MAPLE technique. The results of the <i>in vitro</i> microbiology assay, which utilized a modified disk diffusion approach and were performed using two fungal strains (<i>Candida albicans</i> American Type Culture Collection (ATCC) 90028 and <i>Candida parapsilosis</i> American Type Culture Collection (ATCC) 22019), revealed that voriconazole was released in an active form from the polyvinylpyrrolidone matrix. The results of this study show that the MAPLE-deposited bioactive thin films have a promising potential for use in designing combination products and devices, such as drug delivery devices, and medical device surfaces with antifungal activity. |
| first_indexed | 2024-12-10T22:36:18Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-10T22:36:18Z |
| publishDate | 2019-02-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-9625335f96634da2863558c52b2934152022-12-22T01:30:54ZengMDPI AGApplied Sciences2076-34172019-02-019478610.3390/app9040786app9040786Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive SurfacesIrina Negut0Anita Ioana Visan1Camelia Popescu2Rodica Cristescu3Anton Ficai4Alexandru Mihai Grumezescu5Mariana C. Chifiriuc6Ryan D. Boehm7Dina Yamaleyeva8Michael Taylor9Roger J. Narayan10Douglas B. Chrisey11Lasers Department, National Institute for Lasers, Plasma & Radiation Physics, P.O. Box MG-36, 077125 Bucharest-Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma & Radiation Physics, P.O. Box MG-36, 077125 Bucharest-Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma & Radiation Physics, P.O. Box MG-36, 077125 Bucharest-Magurele, RomaniaLasers Department, National Institute for Lasers, Plasma & Radiation Physics, P.O. Box MG-36, 077125 Bucharest-Magurele, RomaniaFaculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaFaculty of Applied Chemistry and Materials Science, Politehnica University of Bucharest, 1–7 Polizu Street, 011061 Bucharest, RomaniaMicrobiology Immunology Department, Faculty of Biology and Research Institute of the University of Bucharest (ICUB), University of Bucharest, 77206 Bucharest, RomaniaDepartment of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USADepartment of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USADepartment of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USADepartment of Biomedical Engineering, University of North Carolina, Chapel Hill, NC 27599, USADepartment of Physics and Engineering Physics, Tulane University, New Orleans, LA 70118, USAWe explored the potential of biomimetic thin films fabricated by means of matrix-assisted pulsed laser evaporation (MAPLE) for releasing combinations of active substances represented by flavonoids (quercetin dihydrate and resveratrol) and antifungal compounds (amphotericin B and voriconazole) embedded in a polyvinylpyrrolidone biopolymer; the antifungal activity of the film components was evaluated using <i>in vitro</i> microbiological assays. Thin films were deposited using a pulsed KrF* excimer laser source which were structurally characterized using atomic force microscopy (AFM) and Fourier transform infrared spectroscopy (FTIR). High-quality thin films with chemical structures similar to dropcast ones were created using an optimum laser fluence of ~80 mJ/cm<sup>2</sup>. Bioactive substances were included within the polymer thin films using the MAPLE technique. The results of the <i>in vitro</i> microbiology assay, which utilized a modified disk diffusion approach and were performed using two fungal strains (<i>Candida albicans</i> American Type Culture Collection (ATCC) 90028 and <i>Candida parapsilosis</i> American Type Culture Collection (ATCC) 22019), revealed that voriconazole was released in an active form from the polyvinylpyrrolidone matrix. The results of this study show that the MAPLE-deposited bioactive thin films have a promising potential for use in designing combination products and devices, such as drug delivery devices, and medical device surfaces with antifungal activity.https://www.mdpi.com/2076-3417/9/4/786flavonoidquercetin dihydrateresveratrolbiopolymermatrix-assisted pulsed laser evaporationantifungal activity |
| spellingShingle | Irina Negut Anita Ioana Visan Camelia Popescu Rodica Cristescu Anton Ficai Alexandru Mihai Grumezescu Mariana C. Chifiriuc Ryan D. Boehm Dina Yamaleyeva Michael Taylor Roger J. Narayan Douglas B. Chrisey Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces Applied Sciences flavonoid quercetin dihydrate resveratrol biopolymer matrix-assisted pulsed laser evaporation antifungal activity |
| title | Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces |
| title_full | Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces |
| title_fullStr | Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces |
| title_full_unstemmed | Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces |
| title_short | Successful Release of Voriconazole and Flavonoids from MAPLE Deposited Bioactive Surfaces |
| title_sort | successful release of voriconazole and flavonoids from maple deposited bioactive surfaces |
| topic | flavonoid quercetin dihydrate resveratrol biopolymer matrix-assisted pulsed laser evaporation antifungal activity |
| url | https://www.mdpi.com/2076-3417/9/4/786 |
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