Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells
Abstract Background Kangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history. This study is to investigate the influence of KFX in the various cellu...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-08-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1917-7 |
| _version_ | 1818923288038998016 |
|---|---|
| author | Yan-Fen Huang Long-Jian Li Si-Qian Gao Yang Chu Jie Niu Fu-Neng Geng Yong-Mei Shen Li-Hua Peng |
| author_facet | Yan-Fen Huang Long-Jian Li Si-Qian Gao Yang Chu Jie Niu Fu-Neng Geng Yong-Mei Shen Li-Hua Peng |
| author_sort | Yan-Fen Huang |
| collection | DOAJ |
| description | Abstract Background Kangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history. This study is to investigate the influence of KFX in the various cellular activities and evaluate the anti-osteoporosis potential of KFX. Methods The influence of the KFX in the cellular activities, including: 1) migration, osteocalcin secretion of osteoblasts; 2) apoptosis of osteoclasts; 3) migration and tube formation of human umbilical vein endothelial cell (HUVEC); and 4) proliferation, cell cycle regulation and migration of bone marrow mesenchymal stem cells (BMSCs), were investigated systematically. Results KFX was shown to significantly 1) Promote of the migration of osteoblasts, HUVEC, and BMSCs; 2) Increase the secretion of osteocalcin and mineralization of osteoblasts; 3) Accelerate the apoptosis of osteoclasts; 4) Stimulate the proliferation and regulate the cell cycle of BMSCs. Conclusion Taken together, these results provide the evidence for the osteogenesis, anti-osteoporosis and angiogenesis effects of KFX, with the mechanism of activating the bone formation through stimulating the osteoblasts and HUVECs, as well as inhibiting the bone absorption by inhibiting the osteoclasts activities. The KFX was definitely shown a promising bone turnover agent with great potential for anti-osteoporosis treatment. |
| first_indexed | 2024-12-20T02:07:03Z |
| format | Article |
| id | doaj.art-962a6c0c11724d1ca434d4e996a3d895 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-20T02:07:03Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-962a6c0c11724d1ca434d4e996a3d8952022-12-21T19:57:11ZengBMCBMC Complementary and Alternative Medicine1472-68822017-08-0117111210.1186/s12906-017-1917-7Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cellsYan-Fen Huang0Long-Jian Li1Si-Qian Gao2Yang Chu3Jie Niu4Fu-Neng Geng5Yong-Mei Shen6Li-Hua Peng7Institute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversitySichuan Engineering Research Center for Medicinal AnimalsGOODDOCTOR Pharmaceutical CompanyInstitute of Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang UniversityAbstract Background Kangfuxin (KFX) is the ethanol extract of Periplaneta americana L, which has been widely used in the Traditional Chinese Medicine for the repair and regeneration of injured organ and tissues with long history. This study is to investigate the influence of KFX in the various cellular activities and evaluate the anti-osteoporosis potential of KFX. Methods The influence of the KFX in the cellular activities, including: 1) migration, osteocalcin secretion of osteoblasts; 2) apoptosis of osteoclasts; 3) migration and tube formation of human umbilical vein endothelial cell (HUVEC); and 4) proliferation, cell cycle regulation and migration of bone marrow mesenchymal stem cells (BMSCs), were investigated systematically. Results KFX was shown to significantly 1) Promote of the migration of osteoblasts, HUVEC, and BMSCs; 2) Increase the secretion of osteocalcin and mineralization of osteoblasts; 3) Accelerate the apoptosis of osteoclasts; 4) Stimulate the proliferation and regulate the cell cycle of BMSCs. Conclusion Taken together, these results provide the evidence for the osteogenesis, anti-osteoporosis and angiogenesis effects of KFX, with the mechanism of activating the bone formation through stimulating the osteoblasts and HUVECs, as well as inhibiting the bone absorption by inhibiting the osteoclasts activities. The KFX was definitely shown a promising bone turnover agent with great potential for anti-osteoporosis treatment.http://link.springer.com/article/10.1186/s12906-017-1917-7Periplaneta americana LAnti-osteoporosisOsteoblastsOsteoclastsHUVECsBMSCs |
| spellingShingle | Yan-Fen Huang Long-Jian Li Si-Qian Gao Yang Chu Jie Niu Fu-Neng Geng Yong-Mei Shen Li-Hua Peng Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells BMC Complementary and Alternative Medicine Periplaneta americana L Anti-osteoporosis Osteoblasts Osteoclasts HUVECs BMSCs |
| title | Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| title_full | Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| title_fullStr | Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| title_full_unstemmed | Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| title_short | Evidence based anti-osteoporosis effects of Periplaneta americana L on osteoblasts, osteoclasts, vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| title_sort | evidence based anti osteoporosis effects of periplaneta americana l on osteoblasts osteoclasts vascular endothelial cells and bone marrow derived mesenchymal stem cells |
| topic | Periplaneta americana L Anti-osteoporosis Osteoblasts Osteoclasts HUVECs BMSCs |
| url | http://link.springer.com/article/10.1186/s12906-017-1917-7 |
| work_keys_str_mv | AT yanfenhuang evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT longjianli evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT siqiangao evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT yangchu evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT jieniu evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT funenggeng evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT yongmeishen evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells AT lihuapeng evidencebasedantiosteoporosiseffectsofperiplanetaamericanalonosteoblastsosteoclastsvascularendothelialcellsandbonemarrowderivedmesenchymalstemcells |