Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging
Exact interaction mechanism between Bax and Bcl-XL, two key Bcl-2 family proteins, is an interesting and controversial issue. Partial acceptor photobleaching-based quantitative fluorescence resonance energy transfer (FRET) measurement, PbFRET, is a widely used FRET quantification method in living ce...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2020-05-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S179354582050011X |
| _version_ | 1818849414107627520 |
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| author | Fangfang Yang Mengyan Du Xiaoping Wang Tongsheng Chen |
| author_facet | Fangfang Yang Mengyan Du Xiaoping Wang Tongsheng Chen |
| author_sort | Fangfang Yang |
| collection | DOAJ |
| description | Exact interaction mechanism between Bax and Bcl-XL, two key Bcl-2 family proteins, is an interesting and controversial issue. Partial acceptor photobleaching-based quantitative fluorescence resonance energy transfer (FRET) measurement, PbFRET, is a widely used FRET quantification method in living cells. In this report, we implemented pixel-to-pixel PbFRET imaging on a wide-field microscope to map the FRET efficiency (E) images of single living HepG2 cells co-expressing CFP-Bax and YFP-Bcl-XL. The E value between CFP-Bax and YFP-Bcl-XL was 4.59% in cytosol and 11.31% on mitochondria, conclusively indicating the direct interaction of the two proteins, and the interaction of the two proteins was strong on mitochondria and modest in cytosol. |
| first_indexed | 2024-12-19T06:32:51Z |
| format | Article |
| id | doaj.art-962c3645e217413292848cf5efd0b319 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-19T06:32:51Z |
| publishDate | 2020-05-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-962c3645e217413292848cf5efd0b3192022-12-21T20:32:19ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052020-05-011332050011-12050011-1110.1142/S179354582050011X10.1142/S179354582050011XInteraction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imagingFangfang Yang0Mengyan Du1Xiaoping Wang2Tongsheng Chen3MOE Key Laboratory of Laser Life Science and College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. ChinaMOE Key Laboratory of Laser Life Science and College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. ChinaDepartment of Pain Management, the First Affiliated Hospital of Jinan University, Guangzhou 510630, P. R. ChinaMOE Key Laboratory of Laser Life Science and College of Biophotonics, South China Normal University, Guangzhou 510631, P. R. ChinaExact interaction mechanism between Bax and Bcl-XL, two key Bcl-2 family proteins, is an interesting and controversial issue. Partial acceptor photobleaching-based quantitative fluorescence resonance energy transfer (FRET) measurement, PbFRET, is a widely used FRET quantification method in living cells. In this report, we implemented pixel-to-pixel PbFRET imaging on a wide-field microscope to map the FRET efficiency (E) images of single living HepG2 cells co-expressing CFP-Bax and YFP-Bcl-XL. The E value between CFP-Bax and YFP-Bcl-XL was 4.59% in cytosol and 11.31% on mitochondria, conclusively indicating the direct interaction of the two proteins, and the interaction of the two proteins was strong on mitochondria and modest in cytosol.http://www.worldscientific.com/doi/pdf/10.1142/S179354582050011Xbaxbcl-xlprotein–protein interactionfret imagingliving cells |
| spellingShingle | Fangfang Yang Mengyan Du Xiaoping Wang Tongsheng Chen Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging Journal of Innovative Optical Health Sciences bax bcl-xl protein–protein interaction fret imaging living cells |
| title | Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging |
| title_full | Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging |
| title_fullStr | Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging |
| title_full_unstemmed | Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging |
| title_short | Interaction between Bax and Bcl-XL proteins confirmed by partial acceptor photobleaching-based FRET imaging |
| title_sort | interaction between bax and bcl xl proteins confirmed by partial acceptor photobleaching based fret imaging |
| topic | bax bcl-xl protein–protein interaction fret imaging living cells |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S179354582050011X |
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