Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures
Summary: Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs)...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671122005471 |
| _version_ | 1828066614657941504 |
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| author | Estefanía Estévez-Priego Martina Moreno-Fina Emanuela Monni Zaal Kokaia Jordi Soriano Daniel Tornero |
| author_facet | Estefanía Estévez-Priego Martina Moreno-Fina Emanuela Monni Zaal Kokaia Jordi Soriano Daniel Tornero |
| author_sort | Estefanía Estévez-Priego |
| collection | DOAJ |
| description | Summary: Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration. |
| first_indexed | 2024-04-10T23:33:03Z |
| format | Article |
| id | doaj.art-962ce87c1e414f1eb010c6a04fda8b20 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-04-10T23:33:03Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-962ce87c1e414f1eb010c6a04fda8b202023-01-12T04:19:04ZengElsevierStem Cell Reports2213-67112023-01-01181205219Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary culturesEstefanía Estévez-Priego0Martina Moreno-Fina1Emanuela Monni2Zaal Kokaia3Jordi Soriano4Daniel Tornero5Departament de Física de la Matèria Condensada, Universtat de Barcelona, 08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain; Corresponding authorDepartament de Física de la Matèria Condensada, Universtat de Barcelona, 08028 Barcelona, SpainLaboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center, Department of Clinical Sciences, Lund University, 22184 Lund, SwedenLaboratory of Stem Cells and Restorative Neurology, Lund Stem Cell Center, Department of Clinical Sciences, Lund University, 22184 Lund, SwedenDepartament de Física de la Matèria Condensada, Universtat de Barcelona, 08028 Barcelona, Spain; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, SpainLaboratory of Neural Stem Cells and Brain Damage, Department of Biomedical Sciences, Institute of Neurosciences, University of Barcelona, 08036 Barcelona, Spain; August Pi i Sunyer Biomedical Research Institute (IDIBAPS), 08036 Barcelona, Spain; Corresponding authorSummary: Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured. We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration.http://www.sciencedirect.com/science/article/pii/S2213671122005471neural stem cellsneuronal networkshuman cellular modelscalcium imagingfunctional connectivityhuman iPSCs |
| spellingShingle | Estefanía Estévez-Priego Martina Moreno-Fina Emanuela Monni Zaal Kokaia Jordi Soriano Daniel Tornero Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures Stem Cell Reports neural stem cells neuronal networks human cellular models calcium imaging functional connectivity human iPSCs |
| title | Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures |
| title_full | Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures |
| title_fullStr | Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures |
| title_full_unstemmed | Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures |
| title_short | Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures |
| title_sort | long term calcium imaging reveals functional development in hipsc derived cultures comparable to human but not rat primary cultures |
| topic | neural stem cells neuronal networks human cellular models calcium imaging functional connectivity human iPSCs |
| url | http://www.sciencedirect.com/science/article/pii/S2213671122005471 |
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