CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE
The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were co...
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| Format: | Article |
| Language: | Indonesian |
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Syiah Kuala University
2020-02-01
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| Series: | Jurnal Kedokteran Hewan |
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| Online Access: | https://jurnal.usk.ac.id/JKH/article/view/12890 |
| _version_ | 1797279000316346368 |
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| author | Mulyadi Adam Gholib Gholib Hafizuddin Hafizuddin Rumi Sahara Zamzami Muhammad Bahi |
| author_facet | Mulyadi Adam Gholib Gholib Hafizuddin Hafizuddin Rumi Sahara Zamzami Muhammad Bahi |
| author_sort | Mulyadi Adam |
| collection | DOAJ |
| description | The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations. |
| first_indexed | 2024-03-07T16:17:04Z |
| format | Article |
| id | doaj.art-962f407f13e645319b82d2d697e3e4c3 |
| institution | Directory Open Access Journal |
| issn | 1978-225X 2502-5600 |
| language | Indonesian |
| last_indexed | 2024-03-07T16:17:04Z |
| publishDate | 2020-02-01 |
| publisher | Syiah Kuala University |
| record_format | Article |
| series | Jurnal Kedokteran Hewan |
| spelling | doaj.art-962f407f13e645319b82d2d697e3e4c32024-03-04T10:29:00ZindSyiah Kuala UniversityJurnal Kedokteran Hewan1978-225X2502-56002020-02-0113410.21157/j.ked.hewan.v13i4.1289010812CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLEMulyadi Adam0Gholib Gholib1Hafizuddin Hafizuddin2Rumi Sahara Zamzami3Muhammad Bahi4Laboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaLaboratory of Physiology, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaLaboratory of Reproduction, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaStudy Program of Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaDepartement of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh, IndonesiaThe objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter (Ø) of follicles: small follicles (Ø 1 - 5 mm), medium follicles (Ø 5 - 8 mm), and large follicles (Ø 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.https://jurnal.usk.ac.id/JKH/article/view/12890acehnese cattlefollicular fluidovariesestradiol |
| spellingShingle | Mulyadi Adam Gholib Gholib Hafizuddin Hafizuddin Rumi Sahara Zamzami Muhammad Bahi CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE Jurnal Kedokteran Hewan acehnese cattle follicular fluid ovaries estradiol |
| title | CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE |
| title_full | CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE |
| title_fullStr | CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE |
| title_full_unstemmed | CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE |
| title_short | CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE |
| title_sort | characteristic of ovarian and estradiol concentrations in the follicular fluid of slaughtered aceh cattle |
| topic | acehnese cattle follicular fluid ovaries estradiol |
| url | https://jurnal.usk.ac.id/JKH/article/view/12890 |
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