The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats
Introduction: Carbon-tetrachloride (CCl4) is well-known to cause liver damage due to severe oxidative stress. Nerol, on the other hand, is a monoterpene that is antioxidant, antiviral, antibacterial, anti-inflammatory, and anxiolytic. This study set out to determine if nerol may be used as a prophyl...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023102738 |
| _version_ | 1797383884531302400 |
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| author | Milon Mondal Jibanananda Bala Kakoli Rani Mondal Sadia Afrin Protyaee Saha Moumita Saha Sarmin Jamaddar Uttam Kumar Roy Chandan Sarkar |
| author_facet | Milon Mondal Jibanananda Bala Kakoli Rani Mondal Sadia Afrin Protyaee Saha Moumita Saha Sarmin Jamaddar Uttam Kumar Roy Chandan Sarkar |
| author_sort | Milon Mondal |
| collection | DOAJ |
| description | Introduction: Carbon-tetrachloride (CCl4) is well-known to cause liver damage due to severe oxidative stress. Nerol, on the other hand, is a monoterpene that is antioxidant, antiviral, antibacterial, anti-inflammatory, and anxiolytic. This study set out to determine if nerol may be used as a prophylactic measure against the oxidative stress mediated hepatic injury caused by CCl4. Materials and methods: For the aim of this experiment, 35 male Sprague-Dawley rats ranging in body weight (BW) from 140 to 180 g were split into five separate groups. With the exception of vehicle control group 1, all experimental rats were subjected to carbon tetrachloride exposure through intra-peritoneal injection at a 0.7 mL/kg body weight dose once a week for 4 weeks (28 days). The treatment groups 3 and 4 received oral administration of nerol at 50 and 100 mg/kg BW for 28 days. In the same time period, the standard control group received 100 mg/kg BW silymarin. Results: Serum hepatic markers, lipid profiles, albumin, globulin, bilirubin, and total protein were all substantially improved in nerol-treated rats in a dose-dependent manner that had been exposed to CCl4 compared to the only CCl4-treated group. Carbon tetrachloride-exposed rats had lower glutathione, superoxide dismutase, and catalase levels and higher thio-barbituric acid reactive substances (TBARS) levels than normal rats. In contrast, administration of nerol shown a significant augmentation in the concentrations of these antioxidant compounds, while concurrently inducing a decline in the levels of TBARS in the hepatic tissue. In a similar vein, the histo-pathological examination yielded further evidence indicating that nerol offered protection to the hepatocyte against damage generated by CCl4. Conclusion: According to the findings of our investigation, nerol has potential as a functional element to shield the liver from harm brought on by ROS that are caused by CCL4. |
| first_indexed | 2024-03-08T21:27:25Z |
| format | Article |
| id | doaj.art-96341fb48a5b49cc9de00d700eb472aa |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T21:27:25Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-96341fb48a5b49cc9de00d700eb472aa2023-12-21T07:35:39ZengElsevierHeliyon2405-84402023-12-01912e23065The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley ratsMilon Mondal0Jibanananda Bala1Kakoli Rani Mondal2Sadia Afrin3Protyaee Saha4Moumita Saha5Sarmin Jamaddar6Uttam Kumar Roy7Chandan Sarkar8Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh; Corresponding author.Department of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, BangladeshPharmacy Discipline, Khulna University, Khulna, 9208, BangladeshDepartment of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, BangladeshDepartment of Pharmaceutical Sciences, North South University, Dhaka, 1229, BangladeshDepartment of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka, 1342, BangladeshDepartment of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, BangladeshDepartment of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, BangladeshDepartment of Pharmacy, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh; Corresponding author.Introduction: Carbon-tetrachloride (CCl4) is well-known to cause liver damage due to severe oxidative stress. Nerol, on the other hand, is a monoterpene that is antioxidant, antiviral, antibacterial, anti-inflammatory, and anxiolytic. This study set out to determine if nerol may be used as a prophylactic measure against the oxidative stress mediated hepatic injury caused by CCl4. Materials and methods: For the aim of this experiment, 35 male Sprague-Dawley rats ranging in body weight (BW) from 140 to 180 g were split into five separate groups. With the exception of vehicle control group 1, all experimental rats were subjected to carbon tetrachloride exposure through intra-peritoneal injection at a 0.7 mL/kg body weight dose once a week for 4 weeks (28 days). The treatment groups 3 and 4 received oral administration of nerol at 50 and 100 mg/kg BW for 28 days. In the same time period, the standard control group received 100 mg/kg BW silymarin. Results: Serum hepatic markers, lipid profiles, albumin, globulin, bilirubin, and total protein were all substantially improved in nerol-treated rats in a dose-dependent manner that had been exposed to CCl4 compared to the only CCl4-treated group. Carbon tetrachloride-exposed rats had lower glutathione, superoxide dismutase, and catalase levels and higher thio-barbituric acid reactive substances (TBARS) levels than normal rats. In contrast, administration of nerol shown a significant augmentation in the concentrations of these antioxidant compounds, while concurrently inducing a decline in the levels of TBARS in the hepatic tissue. In a similar vein, the histo-pathological examination yielded further evidence indicating that nerol offered protection to the hepatocyte against damage generated by CCl4. Conclusion: According to the findings of our investigation, nerol has potential as a functional element to shield the liver from harm brought on by ROS that are caused by CCL4.http://www.sciencedirect.com/science/article/pii/S2405844023102738NerolCarbon-tetrachlorideHepatotoxicityOxidative-stressHepatoprotective |
| spellingShingle | Milon Mondal Jibanananda Bala Kakoli Rani Mondal Sadia Afrin Protyaee Saha Moumita Saha Sarmin Jamaddar Uttam Kumar Roy Chandan Sarkar The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats Heliyon Nerol Carbon-tetrachloride Hepatotoxicity Oxidative-stress Hepatoprotective |
| title | The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats |
| title_full | The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats |
| title_fullStr | The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats |
| title_full_unstemmed | The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats |
| title_short | The protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in Sprague-Dawley rats |
| title_sort | protective effects of nerol to prevent the toxicity of carbon tetrachloride to the liver in sprague dawley rats |
| topic | Nerol Carbon-tetrachloride Hepatotoxicity Oxidative-stress Hepatoprotective |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023102738 |
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