Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system
This study introduces a novel approach for CO2 reduction to formate using the recombinant formate dehydrogenase 1 (MeFDH1) from Methylorubrum extorquens AM1 as biocatalyst, addressing challenges in activity, productivity, and long-term stability of enzyme. We demonstrate that immobilized MeFDH1 supp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Journal of CO2 Utilization |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212982024000891 |
| _version_ | 1827274303311183872 |
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| author | Byoung Wook Jeon Uyen Thu Phan Yoonyoung Heo Hyung Ho Lee Jungki Ryu Yong Hwan Kim |
| author_facet | Byoung Wook Jeon Uyen Thu Phan Yoonyoung Heo Hyung Ho Lee Jungki Ryu Yong Hwan Kim |
| author_sort | Byoung Wook Jeon |
| collection | DOAJ |
| description | This study introduces a novel approach for CO2 reduction to formate using the recombinant formate dehydrogenase 1 (MeFDH1) from Methylorubrum extorquens AM1 as biocatalyst, addressing challenges in activity, productivity, and long-term stability of enzyme. We demonstrate that immobilized MeFDH1 supported by electrochemical reaction system enhances formate production and stability, achieving over 1.7 M concentration with an initial rate of 20 mM/h and near-unity Faradaic efficiency for over 200 hours. In further, the reusability of immobilized MeFDH1 was obtained without significant declination of productivity and selectivity. The electrochemical study of MeFDH1 found the product inhibition in continuous CO2 conversion. To overcome this challenge and build efficient process, the integration of a flow reactor system and in-situ separation unit further improved the system's performance and scalability. This advancement in enzymatic CO2 conversion suggests the potential of biocatalysis towards addressing global warming through sustainable chemical synthesis. |
| first_indexed | 2024-04-24T16:49:42Z |
| format | Article |
| id | doaj.art-96350412135842e1a26cd818c668fef9 |
| institution | Directory Open Access Journal |
| issn | 2212-9839 |
| language | English |
| last_indexed | 2025-03-22T06:22:52Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of CO2 Utilization |
| spelling | doaj.art-96350412135842e1a26cd818c668fef92024-04-26T04:59:17ZengElsevierJournal of CO2 Utilization2212-98392024-04-0182102754Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor systemByoung Wook Jeon0Uyen Thu Phan1Yoonyoung Heo2Hyung Ho Lee3Jungki Ryu4Yong Hwan Kim5School of Energy and Chemical Engineering and Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of KoreaSchool of Energy and Chemical Engineering and Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of KoreaDepartment of Chemistry, Department of Energy Engineering, College of Natural Sciences, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, Republic of KoreaDepartment of Chemistry, Department of Energy Engineering, College of Natural Sciences, Seoul National University, 1, Gwanak-ro, Gwanak-gu, Seoul, Republic of KoreaSchool of Energy and Chemical Engineering and Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of Korea; Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of Korea; Corresponding author at: School of Energy and Chemical Engineering and Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of Korea.School of Energy and Chemical Engineering and Graduate School of Carbon Neutrality, Ulsan National Institute of Science and Technology, 50, UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, Republic of Korea; Corresponding author.This study introduces a novel approach for CO2 reduction to formate using the recombinant formate dehydrogenase 1 (MeFDH1) from Methylorubrum extorquens AM1 as biocatalyst, addressing challenges in activity, productivity, and long-term stability of enzyme. We demonstrate that immobilized MeFDH1 supported by electrochemical reaction system enhances formate production and stability, achieving over 1.7 M concentration with an initial rate of 20 mM/h and near-unity Faradaic efficiency for over 200 hours. In further, the reusability of immobilized MeFDH1 was obtained without significant declination of productivity and selectivity. The electrochemical study of MeFDH1 found the product inhibition in continuous CO2 conversion. To overcome this challenge and build efficient process, the integration of a flow reactor system and in-situ separation unit further improved the system's performance and scalability. This advancement in enzymatic CO2 conversion suggests the potential of biocatalysis towards addressing global warming through sustainable chemical synthesis.http://www.sciencedirect.com/science/article/pii/S2212982024000891Methylorubrum extorquens AM1Formate dehydrogenase 1 (MeFDH1)Enzymatic CO2 conversionFlow reactorin-situ separation |
| spellingShingle | Byoung Wook Jeon Uyen Thu Phan Yoonyoung Heo Hyung Ho Lee Jungki Ryu Yong Hwan Kim Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system Journal of CO2 Utilization Methylorubrum extorquens AM1 Formate dehydrogenase 1 (MeFDH1) Enzymatic CO2 conversion Flow reactor in-situ separation |
| title | Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system |
| title_full | Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system |
| title_fullStr | Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system |
| title_full_unstemmed | Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system |
| title_short | Enzymatic conversion of CO2 to formate: The potential of tungsten-containing formate dehydrogenase in flow reactor system |
| title_sort | enzymatic conversion of co2 to formate the potential of tungsten containing formate dehydrogenase in flow reactor system |
| topic | Methylorubrum extorquens AM1 Formate dehydrogenase 1 (MeFDH1) Enzymatic CO2 conversion Flow reactor in-situ separation |
| url | http://www.sciencedirect.com/science/article/pii/S2212982024000891 |
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