The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)
Abstract Inducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in dicotyledonous model plants such as Arabidopsis, it has not...
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03241-w |
| _version_ | 1818825849393119232 |
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| author | Marketa Samalova Ian Moore |
| author_facet | Marketa Samalova Ian Moore |
| author_sort | Marketa Samalova |
| collection | DOAJ |
| description | Abstract Inducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in dicotyledonous model plants such as Arabidopsis, it has not been optimised for use with the monocotyledonous model species, namely rice. We have adapted the dexamethasone-inducible pOp6/LhGR system for rice and the results indicated that it is fast, sensitive and tightly regulated, with high levels of induction that remain stable over several generations. Most importantly, we have shown that the system does not cause negative growth defects in vitro or in soil grown plants. Interestingly in the process of testing, we found that another steroid, triamcinolone acetonide, is a more potent inducer in rice than dexamethasone. We present serious considerations for the construct design to avoid undesirable effects caused by the system in plants, leakiness and possible silencing, as well as simple steps to maximize translation efficiency of a gene of interest. Finally, we compare the performance of the pOp6/LhGR system with other chemically inducible systems tested in rice in terms of the properties of an ideal inducible system. |
| first_indexed | 2024-12-19T00:18:18Z |
| format | Article |
| id | doaj.art-9636501052c44554bda64a1e64c9c8b1 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-19T00:18:18Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-9636501052c44554bda64a1e64c9c8b12022-12-21T20:45:40ZengBMCBMC Plant Biology1471-22292021-10-0121111510.1186/s12870-021-03241-wThe steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)Marketa Samalova0Ian Moore1Department of Experimental Biology, Masaryk UniversityDepartment of Plant Sciences, Oxford UniversityAbstract Inducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in dicotyledonous model plants such as Arabidopsis, it has not been optimised for use with the monocotyledonous model species, namely rice. We have adapted the dexamethasone-inducible pOp6/LhGR system for rice and the results indicated that it is fast, sensitive and tightly regulated, with high levels of induction that remain stable over several generations. Most importantly, we have shown that the system does not cause negative growth defects in vitro or in soil grown plants. Interestingly in the process of testing, we found that another steroid, triamcinolone acetonide, is a more potent inducer in rice than dexamethasone. We present serious considerations for the construct design to avoid undesirable effects caused by the system in plants, leakiness and possible silencing, as well as simple steps to maximize translation efficiency of a gene of interest. Finally, we compare the performance of the pOp6/LhGR system with other chemically inducible systems tested in rice in terms of the properties of an ideal inducible system.https://doi.org/10.1186/s12870-021-03241-wDexamethasoneGlucocorticoidChemically inducible gene expressionGVGXVEPromotor |
| spellingShingle | Marketa Samalova Ian Moore The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) BMC Plant Biology Dexamethasone Glucocorticoid Chemically inducible gene expression GVG XVE Promotor |
| title | The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) |
| title_full | The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) |
| title_fullStr | The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) |
| title_full_unstemmed | The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) |
| title_short | The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa) |
| title_sort | steroid inducible pop6 lhgr gene expression system is fast sensitive and does not cause plant growth defects in rice oryza sativa |
| topic | Dexamethasone Glucocorticoid Chemically inducible gene expression GVG XVE Promotor |
| url | https://doi.org/10.1186/s12870-021-03241-w |
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