Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media
Biocatalytic cascades play a fundamental role in sustainable chemical synthesis. Fusion enzymes are one of the powerful toolboxes to enable the tailored combination of multiple enzymes for efficient cooperative cascades. Especially, this approach offers a substantial potential for the practical appl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.944226/full |
| _version_ | 1818189252336287744 |
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| author | Yu Ma Ningning Zhang Guillem Vernet Selin Kara Selin Kara |
| author_facet | Yu Ma Ningning Zhang Guillem Vernet Selin Kara Selin Kara |
| author_sort | Yu Ma |
| collection | DOAJ |
| description | Biocatalytic cascades play a fundamental role in sustainable chemical synthesis. Fusion enzymes are one of the powerful toolboxes to enable the tailored combination of multiple enzymes for efficient cooperative cascades. Especially, this approach offers a substantial potential for the practical application of cofactor-dependent oxidoreductases by forming cofactor self-sufficient cascades. Adequate cofactor recycling while keeping the oxidized/reduced cofactor in a confined microenvironment benefits from the fusion fashion and makes the use of oxidoreductases in harsh non-aqueous media practical. In this mini-review, we have summarized the application of various fusion enzymes in aqueous and non-aqueous media with a focus on the discussion of linker design within oxidoreductases. The design and properties of the reported linkers have been reviewed in detail. Besides, the substrate loadings in these studies have been listed to showcase one of the key limitations (low solubility of hydrophobic substrates) of aqueous biocatalysis when it comes to efficiency and economic feasibility. Therefore, a straightforward strategy of applying non-aqueous media has been briefly discussed while the potential of using the fusion oxidoreductase of interest in organic media was highlighted. |
| first_indexed | 2024-12-11T23:39:52Z |
| format | Article |
| id | doaj.art-963779e4e8554f96829762d5ad0fd38e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-11T23:39:52Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-963779e4e8554f96829762d5ad0fd38e2022-12-22T00:45:46ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-07-011010.3389/fbioe.2022.944226944226Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous mediaYu Ma0Ningning Zhang1Guillem Vernet2Selin Kara3Selin Kara4Biocatalysis and Bioprocessing Group, Department of Biological and Chemical Engineering, Aarhus University, Aarhus, DenmarkInstitute of Technical Chemistry, Leibniz University Hannover, Hannover, GermanyInstitute of Technical Chemistry, Leibniz University Hannover, Hannover, GermanyBiocatalysis and Bioprocessing Group, Department of Biological and Chemical Engineering, Aarhus University, Aarhus, DenmarkInstitute of Technical Chemistry, Leibniz University Hannover, Hannover, GermanyBiocatalytic cascades play a fundamental role in sustainable chemical synthesis. Fusion enzymes are one of the powerful toolboxes to enable the tailored combination of multiple enzymes for efficient cooperative cascades. Especially, this approach offers a substantial potential for the practical application of cofactor-dependent oxidoreductases by forming cofactor self-sufficient cascades. Adequate cofactor recycling while keeping the oxidized/reduced cofactor in a confined microenvironment benefits from the fusion fashion and makes the use of oxidoreductases in harsh non-aqueous media practical. In this mini-review, we have summarized the application of various fusion enzymes in aqueous and non-aqueous media with a focus on the discussion of linker design within oxidoreductases. The design and properties of the reported linkers have been reviewed in detail. Besides, the substrate loadings in these studies have been listed to showcase one of the key limitations (low solubility of hydrophobic substrates) of aqueous biocatalysis when it comes to efficiency and economic feasibility. Therefore, a straightforward strategy of applying non-aqueous media has been briefly discussed while the potential of using the fusion oxidoreductase of interest in organic media was highlighted.https://www.frontiersin.org/articles/10.3389/fbioe.2022.944226/fullfusion enzymesbiocatalytic cascadesoxidoreductasesfusion linkersaqueous and non-aqueous media |
| spellingShingle | Yu Ma Ningning Zhang Guillem Vernet Selin Kara Selin Kara Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media Frontiers in Bioengineering and Biotechnology fusion enzymes biocatalytic cascades oxidoreductases fusion linkers aqueous and non-aqueous media |
| title | Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media |
| title_full | Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media |
| title_fullStr | Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media |
| title_full_unstemmed | Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media |
| title_short | Design of fusion enzymes for biocatalytic applications in aqueous and non-aqueous media |
| title_sort | design of fusion enzymes for biocatalytic applications in aqueous and non aqueous media |
| topic | fusion enzymes biocatalytic cascades oxidoreductases fusion linkers aqueous and non-aqueous media |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.944226/full |
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