Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant
In order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root gr...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S014765132031352X |
| _version_ | 1818458708970045440 |
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| author | Kate Samardzic Joel R. Steele Jake P. Violi Anne Colville Simon M. Mitrovic Kenneth J. Rodgers |
| author_facet | Kate Samardzic Joel R. Steele Jake P. Violi Anne Colville Simon M. Mitrovic Kenneth J. Rodgers |
| author_sort | Kate Samardzic |
| collection | DOAJ |
| description | In order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root growth was significantly inhibited by both treatments. The content of derivatised free and protein-bound BMAA and DAB in seedlings was then analysed by LC-MS/MS. Both NPAAs were detected in free and protein-bound fractions with higher levels detected in free fractions. Compared to shoots, there was approximately tenfold more BMAA and DAB in alfalfa roots. These results suggest that NPAAs might be taken up into crop plants from contaminated irrigation water and enter the food chain. This may present an exposure pathway for NPAAs in humans. |
| first_indexed | 2024-12-14T23:02:46Z |
| format | Article |
| id | doaj.art-9637d70bc2234948b290cdaea18cdc4d |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-12-14T23:02:46Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-9637d70bc2234948b290cdaea18cdc4d2022-12-21T22:44:25ZengElsevierEcotoxicology and Environmental Safety0147-65132021-01-01208111515Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plantKate Samardzic0Joel R. Steele1Jake P. Violi2Anne Colville3Simon M. Mitrovic4Kenneth J. Rodgers5Corresponding author.; School of Life Sciences, University of Technology Sydney, Ultimo, AustraliaSchool of Life Sciences, University of Technology Sydney, Ultimo, AustraliaSchool of Life Sciences, University of Technology Sydney, Ultimo, AustraliaSchool of Life Sciences, University of Technology Sydney, Ultimo, AustraliaSchool of Life Sciences, University of Technology Sydney, Ultimo, AustraliaSchool of Life Sciences, University of Technology Sydney, Ultimo, AustraliaIn order to study the toxicity of the cyanobacterial non-protein amino acids (NPAAs) L-β-N-methylamino-L-alanine (BMAA) and its structural isomer L-2,4-diaminobutyric acid (DAB) in the forage crop plant alfalfa (Medicago sativa), seedlings were exposed to NPAA-containing media for four days. Root growth was significantly inhibited by both treatments. The content of derivatised free and protein-bound BMAA and DAB in seedlings was then analysed by LC-MS/MS. Both NPAAs were detected in free and protein-bound fractions with higher levels detected in free fractions. Compared to shoots, there was approximately tenfold more BMAA and DAB in alfalfa roots. These results suggest that NPAAs might be taken up into crop plants from contaminated irrigation water and enter the food chain. This may present an exposure pathway for NPAAs in humans.http://www.sciencedirect.com/science/article/pii/S014765132031352XBMAADABNon-protein amino acidCyanobacteria |
| spellingShingle | Kate Samardzic Joel R. Steele Jake P. Violi Anne Colville Simon M. Mitrovic Kenneth J. Rodgers Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant Ecotoxicology and Environmental Safety BMAA DAB Non-protein amino acid Cyanobacteria |
| title | Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant |
| title_full | Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant |
| title_fullStr | Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant |
| title_full_unstemmed | Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant |
| title_short | Toxicity and bioaccumulation of two non-protein amino acids synthesised by cyanobacteria, β-N-Methylamino-L-alanine (BMAA) and 2,4-diaminobutyric acid (DAB), on a crop plant |
| title_sort | toxicity and bioaccumulation of two non protein amino acids synthesised by cyanobacteria β n methylamino l alanine bmaa and 2 4 diaminobutyric acid dab on a crop plant |
| topic | BMAA DAB Non-protein amino acid Cyanobacteria |
| url | http://www.sciencedirect.com/science/article/pii/S014765132031352X |
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