Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels
The optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels were carried out. The substrate was characterized using proximate analysis and hydrolysis. The process was optimized using response surface methodology while the kinetics of the hydrolysis was studied using M...
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Format: | Article |
Language: | English |
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KeAi Communications Co., Ltd.
2020-11-01
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Series: | Journal of Bioresources and Bioproducts |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2369969820301183 |
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author | Michael U. Ude Ike Oluka Paul C. Eze |
author_facet | Michael U. Ude Ike Oluka Paul C. Eze |
author_sort | Michael U. Ude |
collection | DOAJ |
description | The optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels were carried out. The substrate was characterized using proximate analysis and hydrolysis. The process was optimized using response surface methodology while the kinetics of the hydrolysis was studied using Michealis-menten model. The results obtained showed that the mixed peels have high hemicellulose content, hydrolysable carbohydrate and cellulose. The optimum conditions for glucose yield of 79% by enzymatic hydrolysis are temperature of 36 °C, time of five days, pH of 4.55 and enzyme dosage of 0.428 g/50 mL. Enzymatic hydrolysis obeyed Michealis-Menten kinetic model with spontaneous and feasible reactions. Therefore, the results have proved that mixed peels (cassava and potato peels) is a good substrate for glucose production and study of other waste peels as feed-stock is recommended. |
first_indexed | 2024-04-09T21:57:34Z |
format | Article |
id | doaj.art-96388e13cdc84b2ba79df047de7bd0f4 |
institution | Directory Open Access Journal |
issn | 2369-9698 |
language | English |
last_indexed | 2024-04-09T21:57:34Z |
publishDate | 2020-11-01 |
publisher | KeAi Communications Co., Ltd. |
record_format | Article |
series | Journal of Bioresources and Bioproducts |
spelling | doaj.art-96388e13cdc84b2ba79df047de7bd0f42023-03-24T04:22:32ZengKeAi Communications Co., Ltd.Journal of Bioresources and Bioproducts2369-96982020-11-0154283290Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peelsMichael U. Ude0Ike Oluka1Paul C. Eze2Engineering Research Development and Production, Projects Development Institute (PRODA), Emene, Enugu State, Nigeria; Corresponding authors.Department of Agricultural and Bioresource Engineering, Enugu State University of Science and Technology (ESUT), Enugu State, NigeriaDepartment of Agricultural and Bioresource Engineering, Enugu State University of Science and Technology (ESUT), Enugu State, NigeriaThe optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels were carried out. The substrate was characterized using proximate analysis and hydrolysis. The process was optimized using response surface methodology while the kinetics of the hydrolysis was studied using Michealis-menten model. The results obtained showed that the mixed peels have high hemicellulose content, hydrolysable carbohydrate and cellulose. The optimum conditions for glucose yield of 79% by enzymatic hydrolysis are temperature of 36 °C, time of five days, pH of 4.55 and enzyme dosage of 0.428 g/50 mL. Enzymatic hydrolysis obeyed Michealis-Menten kinetic model with spontaneous and feasible reactions. Therefore, the results have proved that mixed peels (cassava and potato peels) is a good substrate for glucose production and study of other waste peels as feed-stock is recommended.http://www.sciencedirect.com/science/article/pii/S2369969820301183Enzymatic hydrolysisOptimizationGlucoseCassava and potato peels |
spellingShingle | Michael U. Ude Ike Oluka Paul C. Eze Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels Journal of Bioresources and Bioproducts Enzymatic hydrolysis Optimization Glucose Cassava and potato peels |
title | Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
title_full | Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
title_fullStr | Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
title_full_unstemmed | Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
title_short | Optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
title_sort | optimization and kinetics of glucose production via enzymatic hydrolysis of mixed peels |
topic | Enzymatic hydrolysis Optimization Glucose Cassava and potato peels |
url | http://www.sciencedirect.com/science/article/pii/S2369969820301183 |
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