Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2
Abstract A potential biosurfactant producing isolate was identified as Bacillus aryabhattai strain ZDY2. Biosurfactant production was enhanced by 2.51-fold through the development of an optimized process using response surface methodology. The optimized culture medium contained crude oil 4.0%, yeast...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-03-01
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| Series: | Journal of Petroleum Exploration and Production Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/s13202-020-00866-9 |
| _version_ | 1811235986924896256 |
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| author | Deepak A. Yaraguppi Zabin K. Bagewadi Uday M. Muddapur Sikandar I. Mulla |
| author_facet | Deepak A. Yaraguppi Zabin K. Bagewadi Uday M. Muddapur Sikandar I. Mulla |
| author_sort | Deepak A. Yaraguppi |
| collection | DOAJ |
| description | Abstract A potential biosurfactant producing isolate was identified as Bacillus aryabhattai strain ZDY2. Biosurfactant production was enhanced by 2.51-fold through the development of an optimized process using response surface methodology. The optimized culture medium contained crude oil 4.0%, yeast extract 0.7% and NaNO3 3.0% that yielded 8.86 g/l of biosurfactant. Biosurfactant was characterized for stability up to 100 °C, at pH 5–10 and in the presence of NaCl concentration up to 8%. Biosurfactant demonstrated antimicrobial activity against Salmonella typhimurium, Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Candida tropicalis. The morphological characterization was carried out by scanning electron microscopy with energy-dispersive X-ray analysis. The Fourier-transform infrared spectroscopy analysis reveals the lipopeptide nature of the biosurfactant produced by B. aryabhattai strain ZDY2. The biosurfactant finds application in healthcare and pharmaceutical industries. |
| first_indexed | 2024-04-12T12:01:32Z |
| format | Article |
| id | doaj.art-96395e8bc9cb4c00b96a10c69b44d36f |
| institution | Directory Open Access Journal |
| issn | 2190-0558 2190-0566 |
| language | English |
| last_indexed | 2024-04-12T12:01:32Z |
| publishDate | 2020-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Petroleum Exploration and Production Technology |
| spelling | doaj.art-96395e8bc9cb4c00b96a10c69b44d36f2022-12-22T03:33:51ZengSpringerOpenJournal of Petroleum Exploration and Production Technology2190-05582190-05662020-03-011062483249810.1007/s13202-020-00866-9Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2Deepak A. Yaraguppi0Zabin K. Bagewadi1Uday M. Muddapur2Sikandar I. Mulla3Department of Biotechnology, KLE Technological UniversityDepartment of Biotechnology, KLE Technological UniversityDepartment of Biotechnology, KLE Technological UniversityDepartment of Biochemistry, School of Applied Sciences, REVA UniversityAbstract A potential biosurfactant producing isolate was identified as Bacillus aryabhattai strain ZDY2. Biosurfactant production was enhanced by 2.51-fold through the development of an optimized process using response surface methodology. The optimized culture medium contained crude oil 4.0%, yeast extract 0.7% and NaNO3 3.0% that yielded 8.86 g/l of biosurfactant. Biosurfactant was characterized for stability up to 100 °C, at pH 5–10 and in the presence of NaCl concentration up to 8%. Biosurfactant demonstrated antimicrobial activity against Salmonella typhimurium, Escherichia coli, Micrococcus luteus, Staphylococcus aureus and Candida tropicalis. The morphological characterization was carried out by scanning electron microscopy with energy-dispersive X-ray analysis. The Fourier-transform infrared spectroscopy analysis reveals the lipopeptide nature of the biosurfactant produced by B. aryabhattai strain ZDY2. The biosurfactant finds application in healthcare and pharmaceutical industries.https://doi.org/10.1007/s13202-020-00866-9BiosurfactantOptimizationAntimicrobialCharacterization |
| spellingShingle | Deepak A. Yaraguppi Zabin K. Bagewadi Uday M. Muddapur Sikandar I. Mulla Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 Journal of Petroleum Exploration and Production Technology Biosurfactant Optimization Antimicrobial Characterization |
| title | Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 |
| title_full | Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 |
| title_fullStr | Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 |
| title_full_unstemmed | Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 |
| title_short | Response surface methodology-based optimization of biosurfactant production from isolated Bacillus aryabhattai strain ZDY2 |
| title_sort | response surface methodology based optimization of biosurfactant production from isolated bacillus aryabhattai strain zdy2 |
| topic | Biosurfactant Optimization Antimicrobial Characterization |
| url | https://doi.org/10.1007/s13202-020-00866-9 |
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