Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens
ABSTRACT: This study investigated the effects of dietary isoleucine (Ile) on growth performance, intestinal expression of amino acid transporters, protein metabolism-related genes and intestinal microbiota in starter phase Chinese yellow-feathered chickens. Female Xinguang yellow-feathered chickens...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-08-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123002936 |
| _version_ | 1797770131184549888 |
|---|---|
| author | D. Ruan Q.L. Fan S. Zhang H.K. EI-Senousey A.M. Fouad X.J. Lin X.L. Dong Y.F. Deng S.J. Yan C.T. Zheng Z.Y. Jiang S.Q. Jiang |
| author_facet | D. Ruan Q.L. Fan S. Zhang H.K. EI-Senousey A.M. Fouad X.J. Lin X.L. Dong Y.F. Deng S.J. Yan C.T. Zheng Z.Y. Jiang S.Q. Jiang |
| author_sort | D. Ruan |
| collection | DOAJ |
| description | ABSTRACT: This study investigated the effects of dietary isoleucine (Ile) on growth performance, intestinal expression of amino acid transporters, protein metabolism-related genes and intestinal microbiota in starter phase Chinese yellow-feathered chickens. Female Xinguang yellow-feathered chickens (n = 1,080, aged 1 d) were randomly distributed to 6 treatments, each with 6 replicates of 30 birds. Chickens were fed diets with 6 levels of total Ile (6.8, 7.6, 8.4, 9.2, 10.0, and 10.8 g/kg) for 30 d. The average daily gain and feed conversion ratio were improved with dietary Ile levels (P < 0.05). Plasma uric acid content and glutamic-oxalacetic transaminase activity were linearly and quadratically decreased with increasing dietary Ile inclusion (P < 0.05). Dietary Ile level had a linear (P < 0.05) or quadratic (P < 0.05) effect on the jejunal expression of ribosomal protein S6 kinase B1 and eukaryotic translation initiation factor 4E binding protein 1. The relative expression of jejunal 20S proteasome subunit C2 and ileal muscle ring finger-containing protein 1 decreased linearly (P < 0.05) and quadratically (P < 0.05) with increasing dietary Ile levels. Dietary Ile level had a linear (P = 0.069) or quadratic (P < 0.05) effect on the gene expression of solute carrier family 15 member 1 in jejunum and solute carrier family 7 member 1 in ileum. In addition, bacterial 16S rDNA full-length sequencing showed that dietary Ile increased the cecal abundances of the Firmicutes phylum, and Blautia, Lactobacillus, and unclassified_Lachnospiraceae genera, while decreased that of Proteobacteria, Alistipes, and Shigella. Dietary Ile levels affected growth performance and modulated gut microbiota in yellow-feathered chickens. The appropriate level of dietary Ile can upregulate the expression of intestinal protein synthesis-related protein kinase genes and concomitantly inhibit the expression of proteolysis-related cathepsin genes. |
| first_indexed | 2024-03-12T21:18:01Z |
| format | Article |
| id | doaj.art-963f387303e247bcb795727424b1c282 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-12T21:18:01Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-963f387303e247bcb795727424b1c2822023-07-29T04:34:17ZengElsevierPoultry Science0032-57912023-08-011028102774Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickensD. Ruan0Q.L. Fan1S. Zhang2H.K. EI-Senousey3A.M. Fouad4X.J. Lin5X.L. Dong6Y.F. Deng7S.J. Yan8C.T. Zheng9Z.Y. Jiang10S.Q. Jiang11Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, EgyptDepartment of Animal Production, Faculty of Agriculture, Cairo University, Giza 12613, EgyptInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaCJ International Trading Co., Ltd., Shanghai 201107, ChinaCJ International Trading Co., Ltd., Shanghai 201107, ChinaGuangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, ChinaInstitute of Animal Science, Guangdong Academy of Agricultural Sciences, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China; Corresponding author:ABSTRACT: This study investigated the effects of dietary isoleucine (Ile) on growth performance, intestinal expression of amino acid transporters, protein metabolism-related genes and intestinal microbiota in starter phase Chinese yellow-feathered chickens. Female Xinguang yellow-feathered chickens (n = 1,080, aged 1 d) were randomly distributed to 6 treatments, each with 6 replicates of 30 birds. Chickens were fed diets with 6 levels of total Ile (6.8, 7.6, 8.4, 9.2, 10.0, and 10.8 g/kg) for 30 d. The average daily gain and feed conversion ratio were improved with dietary Ile levels (P < 0.05). Plasma uric acid content and glutamic-oxalacetic transaminase activity were linearly and quadratically decreased with increasing dietary Ile inclusion (P < 0.05). Dietary Ile level had a linear (P < 0.05) or quadratic (P < 0.05) effect on the jejunal expression of ribosomal protein S6 kinase B1 and eukaryotic translation initiation factor 4E binding protein 1. The relative expression of jejunal 20S proteasome subunit C2 and ileal muscle ring finger-containing protein 1 decreased linearly (P < 0.05) and quadratically (P < 0.05) with increasing dietary Ile levels. Dietary Ile level had a linear (P = 0.069) or quadratic (P < 0.05) effect on the gene expression of solute carrier family 15 member 1 in jejunum and solute carrier family 7 member 1 in ileum. In addition, bacterial 16S rDNA full-length sequencing showed that dietary Ile increased the cecal abundances of the Firmicutes phylum, and Blautia, Lactobacillus, and unclassified_Lachnospiraceae genera, while decreased that of Proteobacteria, Alistipes, and Shigella. Dietary Ile levels affected growth performance and modulated gut microbiota in yellow-feathered chickens. The appropriate level of dietary Ile can upregulate the expression of intestinal protein synthesis-related protein kinase genes and concomitantly inhibit the expression of proteolysis-related cathepsin genes.http://www.sciencedirect.com/science/article/pii/S0032579123002936isoleucineyellow-feathered chickensamino acid transportersprotein metabolismgut microbiota |
| spellingShingle | D. Ruan Q.L. Fan S. Zhang H.K. EI-Senousey A.M. Fouad X.J. Lin X.L. Dong Y.F. Deng S.J. Yan C.T. Zheng Z.Y. Jiang S.Q. Jiang Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens Poultry Science isoleucine yellow-feathered chickens amino acid transporters protein metabolism gut microbiota |
| title | Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens |
| title_full | Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens |
| title_fullStr | Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens |
| title_full_unstemmed | Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens |
| title_short | Dietary isoleucine supplementation enhances growth performance, modulates the expression of genes related to amino acid transporters and protein metabolism, and gut microbiota in yellow-feathered chickens |
| title_sort | dietary isoleucine supplementation enhances growth performance modulates the expression of genes related to amino acid transporters and protein metabolism and gut microbiota in yellow feathered chickens |
| topic | isoleucine yellow-feathered chickens amino acid transporters protein metabolism gut microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123002936 |
| work_keys_str_mv | AT druan dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT qlfan dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT szhang dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT hkeisenousey dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT amfouad dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT xjlin dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT xldong dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT yfdeng dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT sjyan dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT ctzheng dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT zyjiang dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens AT sqjiang dietaryisoleucinesupplementationenhancesgrowthperformancemodulatestheexpressionofgenesrelatedtoaminoacidtransportersandproteinmetabolismandgutmicrobiotainyellowfeatheredchickens |