Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.
The procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum infiltration with Agrobacterium rhizogenes A4....
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
International Journal of Secondary Metabolite
2018-12-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/39967/478267?publisher=ijate |
| _version_ | 1828023489623228416 |
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| author | Olha Mikolaivna Yaroshko Mykola Vyktorovych Kuchuk |
| author_facet | Olha Mikolaivna Yaroshko Mykola Vyktorovych Kuchuk |
| author_sort | Olha Mikolaivna Yaroshko |
| collection | DOAJ |
| description | The procedure for vacuum infiltration of cultivars
A. caudatus L. and hybrids of A. caudatus
L. xA. paniculatus L. was
optimized. The functioning of
gene construction pCBv19 in the Amaranthus
leaves was evaluated by the transient expression after vacuum infiltration with
Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties
of amaranth species A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh,
A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the
roots are confirmed by the results of the PCR analysis. |
| first_indexed | 2024-04-10T12:29:06Z |
| format | Article |
| id | doaj.art-9640083de0674b019688212ac6cdb41a |
| institution | Directory Open Access Journal |
| issn | 2148-6905 |
| language | English |
| last_indexed | 2024-04-10T12:29:06Z |
| publishDate | 2018-12-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj.art-9640083de0674b019688212ac6cdb41a2023-02-15T16:15:03ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052018-12-015431231810.21448/ijsm.478267618Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L.Olha Mikolaivna YaroshkoMykola Vyktorovych KuchukThe procedure for vacuum infiltration of cultivars A. caudatus L. and hybrids of A. caudatus L. xA. paniculatus L. was optimized. The functioning of gene construction pCBv19 in the Amaranthus leaves was evaluated by the transient expression after vacuum infiltration with Agrobacterium rhizogenes A4. After hypocotyl transformation of the varieties of amaranth species A. caudatus L.: Helios, Karmin, Kremovyi rannii, and hybrids A. caudatus x A. paniculatus L. – cv. Sterkh, A. caudatus x Sterkh- cv. Zhaivir with the wild strain A. rhizogenes A4, the culture of "hairy roots" was obtained. Embedding and transcription of genes in the roots are confirmed by the results of the PCR analysis.https://dergipark.org.tr/tr/pub/ijsm/issue/39967/478267?publisher=ijateamaranthusagrobacteriumtransformationtransgenic roots |
| spellingShingle | Olha Mikolaivna Yaroshko Mykola Vyktorovych Kuchuk Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. International Journal of Secondary Metabolite amaranthus agrobacterium transformation transgenic roots |
| title | Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. |
| title_full | Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. |
| title_fullStr | Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. |
| title_full_unstemmed | Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. |
| title_short | Agrobacterium – caused transformation of cultivars Amaranthus caudatus L. and hybrids of A. caudatus L. x A. paniculatus L. |
| title_sort | agrobacterium caused transformation of cultivars amaranthus caudatus l and hybrids of a caudatus l x a paniculatus l |
| topic | amaranthus agrobacterium transformation transgenic roots |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/39967/478267?publisher=ijate |
| work_keys_str_mv | AT olhamikolaivnayaroshko agrobacteriumcausedtransformationofcultivarsamaranthuscaudatuslandhybridsofacaudatuslxapaniculatusl AT mykolavyktorovychkuchuk agrobacteriumcausedtransformationofcultivarsamaranthuscaudatuslandhybridsofacaudatuslxapaniculatusl |