COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS
Hydroxyapatite materials prepared by two methods: synthetic (HA–S) and bovine (HA-B) granules were exposed to a longterm in vitro test under dynamic conditions. Testing cells, filled up to one fourth (¼V) of their volume with the tested material, were exposed to continuous flow of simulated body flu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Chemistry and Technology, Prague
2014-03-01
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| Series: | Ceramics-Silikáty |
| Subjects: | |
| Online Access: | http://www.ceramics-silikaty.cz/2014/pdf/2014_01_070.pdf |
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| author | DIANA HORKAVCOVÁ DANA ROHANOVÁ LENKA KUNCOVÁ KLÁRA ZÍTKOVÁ ZUZANA ZLÁMALOVÁ CÍLOVÁ ALEŠ HELEBRANT |
| author_facet | DIANA HORKAVCOVÁ DANA ROHANOVÁ LENKA KUNCOVÁ KLÁRA ZÍTKOVÁ ZUZANA ZLÁMALOVÁ CÍLOVÁ ALEŠ HELEBRANT |
| author_sort | DIANA HORKAVCOVÁ |
| collection | DOAJ |
| description | Hydroxyapatite materials prepared by two methods: synthetic (HA–S) and bovine (HA-B) granules were exposed to a longterm in vitro test under dynamic conditions. Testing cells, filled up to one fourth (¼V) of their volume with the tested material, were exposed to continuous flow of simulated body fluid (SBF) for 56 days. The objective of the experiment was to determine whether reactivity of the biomaterials (hydroxyapatites), prepared by different methods but identical in terms of their chemical and phase composition, in SBF were comparable. Analyses of the solutions proved that both materials were highly reactive from the very beginning of interaction with SBF (significant decrease of Ca2+ and (PO4)3- concentrations in the leachate). SEM/EDS images have shown that the surface of bovine HA-B was covered with a new hydroxyapatite (HAp) phase in the first two weeks of the test while synthetic HA–S was covered after two weeks of the immersion in SBF. At the end of the test, day 56, both materials were completely covered with well developed porous HAp phase in form of nano-plates. A calculation of the rate of HAp formation from the concentration of (PO4)3- ions in SBF leachates confirmed that all removed ions were consumed for the formation of the HAp phase throughout the entire testing time for bovine HA–B and only during the second half of the testing time for synthetic HA–S. |
| first_indexed | 2024-12-20T04:48:54Z |
| format | Article |
| id | doaj.art-9640c7c6a06f47c2bbe9f1773d9bdc91 |
| institution | Directory Open Access Journal |
| issn | 0862-5468 1804-5847 |
| language | English |
| last_indexed | 2024-12-20T04:48:54Z |
| publishDate | 2014-03-01 |
| publisher | University of Chemistry and Technology, Prague |
| record_format | Article |
| series | Ceramics-Silikáty |
| spelling | doaj.art-9640c7c6a06f47c2bbe9f1773d9bdc912022-12-21T19:52:54ZengUniversity of Chemistry and Technology, PragueCeramics-Silikáty0862-54681804-58472014-03-015817078COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONSDIANA HORKAVCOVÁ0DANA ROHANOVÁ1LENKA KUNCOVÁ2KLÁRA ZÍTKOVÁ3ZUZANA ZLÁMALOVÁ CÍLOVÁ4ALEŠ HELEBRANT5Department of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicDepartment of Glass and Ceramics, Institute of Chemical Technology, Technická 5, 166 28 Prague 6, Czech RepublicHydroxyapatite materials prepared by two methods: synthetic (HA–S) and bovine (HA-B) granules were exposed to a longterm in vitro test under dynamic conditions. Testing cells, filled up to one fourth (¼V) of their volume with the tested material, were exposed to continuous flow of simulated body fluid (SBF) for 56 days. The objective of the experiment was to determine whether reactivity of the biomaterials (hydroxyapatites), prepared by different methods but identical in terms of their chemical and phase composition, in SBF were comparable. Analyses of the solutions proved that both materials were highly reactive from the very beginning of interaction with SBF (significant decrease of Ca2+ and (PO4)3- concentrations in the leachate). SEM/EDS images have shown that the surface of bovine HA-B was covered with a new hydroxyapatite (HAp) phase in the first two weeks of the test while synthetic HA–S was covered after two weeks of the immersion in SBF. At the end of the test, day 56, both materials were completely covered with well developed porous HAp phase in form of nano-plates. A calculation of the rate of HAp formation from the concentration of (PO4)3- ions in SBF leachates confirmed that all removed ions were consumed for the formation of the HAp phase throughout the entire testing time for bovine HA–B and only during the second half of the testing time for synthetic HA–S.http://www.ceramics-silikaty.cz/2014/pdf/2014_01_070.pdfHydroxyapatitein vitro testSBF |
| spellingShingle | DIANA HORKAVCOVÁ DANA ROHANOVÁ LENKA KUNCOVÁ KLÁRA ZÍTKOVÁ ZUZANA ZLÁMALOVÁ CÍLOVÁ ALEŠ HELEBRANT COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS Ceramics-Silikáty Hydroxyapatite in vitro test SBF |
| title | COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS |
| title_full | COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS |
| title_fullStr | COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS |
| title_full_unstemmed | COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS |
| title_short | COMPARISON OF REACTIVITY OF SYNTHETIC AND BOVINE HYDROXYAPATITE IN VITRO UNDER DYNAMIC CONDITIONS |
| title_sort | comparison of reactivity of synthetic and bovine hydroxyapatite in vitro under dynamic conditions |
| topic | Hydroxyapatite in vitro test SBF |
| url | http://www.ceramics-silikaty.cz/2014/pdf/2014_01_070.pdf |
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