Metabolomic credentialing of murine carcinogen-induced urothelial cancer
Abstract Bladder cancer (BCa) is the most common malignancy of the urinary system with increasing incidence, mortality, and limited treatment options. Therefore, it is imperative to validate preclinical models that faithfully represent BCa cellular, molecular, and metabolic heterogeneity to develop...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-99746-3 |
| _version_ | 1798034866246254592 |
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| author | Hesham Afify Alia Ghoneum Sameh Almousa Ammar Yasser Abdulfattah Bailey Warren Kendall Langsten Daniela Gonzalez Randy Casals Manish Bharadwaj Steven Kridel Neveen Said |
| author_facet | Hesham Afify Alia Ghoneum Sameh Almousa Ammar Yasser Abdulfattah Bailey Warren Kendall Langsten Daniela Gonzalez Randy Casals Manish Bharadwaj Steven Kridel Neveen Said |
| author_sort | Hesham Afify |
| collection | DOAJ |
| description | Abstract Bladder cancer (BCa) is the most common malignancy of the urinary system with increasing incidence, mortality, and limited treatment options. Therefore, it is imperative to validate preclinical models that faithfully represent BCa cellular, molecular, and metabolic heterogeneity to develop new therapeutics. We performed metabolomic profiling of premalignant and non-muscle invasive bladder cancer (NMIBC) that ensued in the chemical carcinogenesis N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) mouse model. We identified the enriched metabolic signatures that associate with premalignant and NMIBC. We found that enrichment of lipid metabolism is the forerunner of carcinogen-induced premalignant and NMIBC lesions. Cross-species analysis revealed the prognostic value of the enzymes associated with carcinogen-induced enriched metabolic in human disease. To date, this is the first study describing the global metabolomic profiles associated with early premalignant and NMIBC and provide evidence that these metabolomic signatures can be used for prognostication of human disease. |
| first_indexed | 2024-04-11T20:50:21Z |
| format | Article |
| id | doaj.art-9642edddfaa74bfa8f06a1f1d2f9bd3a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T20:50:21Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-9642edddfaa74bfa8f06a1f1d2f9bd3a2022-12-22T04:03:53ZengNature PortfolioScientific Reports2045-23222021-11-0111111310.1038/s41598-021-99746-3Metabolomic credentialing of murine carcinogen-induced urothelial cancerHesham Afify0Alia Ghoneum1Sameh Almousa2Ammar Yasser Abdulfattah3Bailey Warren4Kendall Langsten5Daniela Gonzalez6Randy Casals7Manish Bharadwaj8Steven Kridel9Neveen Said10Department of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineCell Analysis Division, Agilent Technologies, IncDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineDepartment of Cancer Biology, Medical Center Boulevard, Wake Forest University School of MedicineAbstract Bladder cancer (BCa) is the most common malignancy of the urinary system with increasing incidence, mortality, and limited treatment options. Therefore, it is imperative to validate preclinical models that faithfully represent BCa cellular, molecular, and metabolic heterogeneity to develop new therapeutics. We performed metabolomic profiling of premalignant and non-muscle invasive bladder cancer (NMIBC) that ensued in the chemical carcinogenesis N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN) mouse model. We identified the enriched metabolic signatures that associate with premalignant and NMIBC. We found that enrichment of lipid metabolism is the forerunner of carcinogen-induced premalignant and NMIBC lesions. Cross-species analysis revealed the prognostic value of the enzymes associated with carcinogen-induced enriched metabolic in human disease. To date, this is the first study describing the global metabolomic profiles associated with early premalignant and NMIBC and provide evidence that these metabolomic signatures can be used for prognostication of human disease.https://doi.org/10.1038/s41598-021-99746-3 |
| spellingShingle | Hesham Afify Alia Ghoneum Sameh Almousa Ammar Yasser Abdulfattah Bailey Warren Kendall Langsten Daniela Gonzalez Randy Casals Manish Bharadwaj Steven Kridel Neveen Said Metabolomic credentialing of murine carcinogen-induced urothelial cancer Scientific Reports |
| title | Metabolomic credentialing of murine carcinogen-induced urothelial cancer |
| title_full | Metabolomic credentialing of murine carcinogen-induced urothelial cancer |
| title_fullStr | Metabolomic credentialing of murine carcinogen-induced urothelial cancer |
| title_full_unstemmed | Metabolomic credentialing of murine carcinogen-induced urothelial cancer |
| title_short | Metabolomic credentialing of murine carcinogen-induced urothelial cancer |
| title_sort | metabolomic credentialing of murine carcinogen induced urothelial cancer |
| url | https://doi.org/10.1038/s41598-021-99746-3 |
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