Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni
The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic dNAs (RAPDs) generated with three primers. Phenograms showing the simil...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
1997-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000300019 |
| _version_ | 1827831758461075456 |
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| author | Patricia Machado Pinto Cristiana FA Brito Liana K Jannoti Passos Miriam Tendler Andrew JG Simpson |
| author_facet | Patricia Machado Pinto Cristiana FA Brito Liana K Jannoti Passos Miriam Tendler Andrew JG Simpson |
| author_sort | Patricia Machado Pinto |
| collection | DOAJ |
| description | The extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic dNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolates |
| first_indexed | 2024-03-12T04:58:53Z |
| format | Article |
| id | doaj.art-964541fba2f84f649a4b582feae26959 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T04:58:53Z |
| publishDate | 1997-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-964541fba2f84f649a4b582feae269592023-09-03T09:14:29ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601997-05-0192340910.1590/S0074-02761997000300019Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoniPatricia Machado PintoCristiana FA BritoLiana K Jannoti PassosMiriam TendlerAndrew JG SimpsonThe extent of genomic variability of clones of Schistosoma mansoni obtained from field isolates was compared with that of strains that have been laboratory maintained. Analysis was undertaken using randomly amplified polymorphic dNAs (RAPDs) generated with three primers. Phenograms showing the similarity among the clones were constructed. The data showed that while the laboratory strain is highly homogeneous the clones derived from the field populations were highly variable with 43% of RAPDs exhibiting polymorphisms among 23 clones. Clones isolated from the same infected individual were always more closely grouped than clones from different individuals. The data clearly demonstrated that earlier analyses of the genomic variability in S. mansoni have underestimated this phenomenon due to the failure to examine field isolateshttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000300019polymerase chain reactionrandomly amplified polymorphic DNAschistosomiasisSchistosoma mansoni |
| spellingShingle | Patricia Machado Pinto Cristiana FA Brito Liana K Jannoti Passos Miriam Tendler Andrew JG Simpson Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni Memorias do Instituto Oswaldo Cruz polymerase chain reaction randomly amplified polymorphic DNA schistosomiasis Schistosoma mansoni |
| title | Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni |
| title_full | Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni |
| title_fullStr | Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni |
| title_full_unstemmed | Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni |
| title_short | Contrasting Genomic Variability between Clones from Field Isolates and Laboratory Populations of Schistosoma mansoni |
| title_sort | contrasting genomic variability between clones from field isolates and laboratory populations of schistosoma mansoni |
| topic | polymerase chain reaction randomly amplified polymorphic DNA schistosomiasis Schistosoma mansoni |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000300019 |
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