Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum
The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of t...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019365521 |
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| author | David Febrilliant Susanto Hakun Wirawasista Aparamarta Arief Widjaja Nurul Jadid Setiyo Gunawan |
| author_facet | David Febrilliant Susanto Hakun Wirawasista Aparamarta Arief Widjaja Nurul Jadid Setiyo Gunawan |
| author_sort | David Febrilliant Susanto |
| collection | DOAJ |
| description | The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS2 as the solvent and crude extract to CS2 mass ratio of 1/10 (g/g). |
| first_indexed | 2024-12-13T12:36:52Z |
| format | Article |
| id | doaj.art-96471169dbb64405a26ca4c8e71cdd9b |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-13T12:36:52Z |
| publishDate | 2019-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-96471169dbb64405a26ca4c8e71cdd9b2022-12-21T23:45:48ZengElsevierHeliyon2405-84402019-11-01511e02893Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllumDavid Febrilliant Susanto0Hakun Wirawasista Aparamarta1Arief Widjaja2Nurul Jadid3Setiyo Gunawan4Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, IndonesiaDepartment of Biology, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, IndonesiaDepartment of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya, 60111, Indonesia; Corresponding author.The highest population of Calophyllum inophyllum is in Indonesia. One of the bioactive compound contained in C. inophyllum leave is cholestane. The extraction method was employed to obtain crude extract from these leaves. It was followed by identification of the bioactive compounds. The purpose of this research was to develop a process for isolating and identifying cholestane and dihydropyrene from methanolic extract of C. inophyllum leaves in high yield and purity. The effect of crude extract to non-polar solvent mass ratio and non-polar solvent types on the separation of the bioactive compounds were also systematically investigated. New compounds (trans-2-[2-(trifluoromethyl)phenyl]-10b,10c-dimethyl-10b, 10c-dihydropyrene and anti-4-aza-B-homo-5.alpha-cholestane-3-one) were also identified in C. inophyllum leaves. The successful separation was obtained by employing CS2 as the solvent and crude extract to CS2 mass ratio of 1/10 (g/g).http://www.sciencedirect.com/science/article/pii/S2405844019365521Chemical engineeringBiochemical engineeringPharmaceutical engineeringTransport processIndustrial chemistryMaterials characterization |
| spellingShingle | David Febrilliant Susanto Hakun Wirawasista Aparamarta Arief Widjaja Nurul Jadid Setiyo Gunawan Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum Heliyon Chemical engineering Biochemical engineering Pharmaceutical engineering Transport process Industrial chemistry Materials characterization |
| title | Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum |
| title_full | Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum |
| title_fullStr | Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum |
| title_full_unstemmed | Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum |
| title_short | Isolation and identification of cholestane and dihydropyrene from Calophyllum inophyllum |
| title_sort | isolation and identification of cholestane and dihydropyrene from calophyllum inophyllum |
| topic | Chemical engineering Biochemical engineering Pharmaceutical engineering Transport process Industrial chemistry Materials characterization |
| url | http://www.sciencedirect.com/science/article/pii/S2405844019365521 |
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