Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine
Abstract In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric det...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2018-11-01
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| Series: | Micro and Nano Systems Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40486-018-0070-5 |
| _version_ | 1819057707840176128 |
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| author | Sayed Zia Mohammadi Hadi Beitollahi Somayeh Tajik |
| author_facet | Sayed Zia Mohammadi Hadi Beitollahi Somayeh Tajik |
| author_sort | Sayed Zia Mohammadi |
| collection | DOAJ |
| description | Abstract In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screen-printed electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1–500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system. |
| first_indexed | 2024-12-21T13:43:36Z |
| format | Article |
| id | doaj.art-9649be78304d4c2d9f8dd78a2eb4d609 |
| institution | Directory Open Access Journal |
| issn | 2213-9621 |
| language | English |
| last_indexed | 2024-12-21T13:43:36Z |
| publishDate | 2018-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Micro and Nano Systems Letters |
| spelling | doaj.art-9649be78304d4c2d9f8dd78a2eb4d6092022-12-21T19:01:57ZengSpringerOpenMicro and Nano Systems Letters2213-96212018-11-01611710.1186/s40486-018-0070-5Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholineSayed Zia Mohammadi0Hadi Beitollahi1Somayeh Tajik2Department of Chemistry, Payame Noor UniversityEnvironment Department, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced TechnologyNanoBioElectrochemistry Research Center, Bam University of Medical SciencesAbstract In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screen-printed electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1–500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system.http://link.springer.com/article/10.1186/s40486-018-0070-5Acetylcholine determinationMagnetic core shell nanoparticlesScreen-printed carbon electrodeVoltammetry |
| spellingShingle | Sayed Zia Mohammadi Hadi Beitollahi Somayeh Tajik Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine Micro and Nano Systems Letters Acetylcholine determination Magnetic core shell nanoparticles Screen-printed carbon electrode Voltammetry |
| title | Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine |
| title_full | Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine |
| title_fullStr | Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine |
| title_full_unstemmed | Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine |
| title_short | Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine |
| title_sort | nonenzymatic coated screen printed electrode for electrochemical determination of acetylcholine |
| topic | Acetylcholine determination Magnetic core shell nanoparticles Screen-printed carbon electrode Voltammetry |
| url | http://link.springer.com/article/10.1186/s40486-018-0070-5 |
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