Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages
Abstract Background The sexual maturity of chickens is an important economic trait, and the breeding of precocious and delayed puberty roosters is an important selection strategy for broilers. The comb serves as an important secondary sexual characteristic of roosters and determines their sexual pre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-024-09960-2 |
| _version_ | 1797363601730699264 |
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| author | Hao Qi Zhidan Deng Fei Ye Junwei Gou Miaoxin Huang Hai Xiang Hua Li |
| author_facet | Hao Qi Zhidan Deng Fei Ye Junwei Gou Miaoxin Huang Hai Xiang Hua Li |
| author_sort | Hao Qi |
| collection | DOAJ |
| description | Abstract Background The sexual maturity of chickens is an important economic trait, and the breeding of precocious and delayed puberty roosters is an important selection strategy for broilers. The comb serves as an important secondary sexual characteristic of roosters and determines their sexual precocity. Moreover, comb development is closely associated with gonad development in roosters. However, the underlying molecular mechanism regulating the sexual maturity of roosters has not yet been fully explored. Results In order to identify the genes related to precocious puberty in Qingyuan partridge roosters, and based on the synchrony of testis and combs development, combined with histological observation and RNA-seq method, the developmental status and gene expression profile of combs and testis were obtained. The results showed that during the early growth and development period (77 days of age), the development of combs and testis was significant in the high comb (H) group versus the low comb (L) group (p < 0.05); however, the morphological characteristic of the comb and testicular tissues converged during the late growth and development period (112 days of age) in the H and L groups. Based on these results, RNA-sequencing analysis was performed on the comb and testis tissues of the 77 and 112 days old Qingyuan Partridge roosters with different comb height traits. GO and KEGG analysis enrichment analysis showed that the differentially expressed genes were primarily enriched in MAPK signaling, VEGF signaling, and retinol metabolism pathways. Moreover, weighted correlation network analysis and module co-expression network analysis identified WNT6, AMH, IHH, STT3A, PEX16, KPNA7, CATHL2, ROR2, PAMR1, WISP2, IL17REL, NDRG4, CYP26B1, and CRHBP as the key genes associated with the regulation of precocity and delayed puberty in Qingyuan Partridge roosters. Conclusions In summary, we identified the key regulatory genes of sexual precocity in roosters, which provide a theoretical basis for understanding the developmental differences between precocious and delayed puberty in roosters. |
| first_indexed | 2024-03-08T16:23:31Z |
| format | Article |
| id | doaj.art-964b21b033ef4970b881efdfaf76c80a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-08T16:23:31Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-964b21b033ef4970b881efdfaf76c80a2024-01-07T12:12:34ZengBMCBMC Genomics1471-21642024-01-0125111610.1186/s12864-024-09960-2Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stagesHao Qi0Zhidan Deng1Fei Ye2Junwei Gou3Miaoxin Huang4Hai Xiang5Hua Li6Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityGuangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding, College of Life Science and Engineering, Foshan UniversityAbstract Background The sexual maturity of chickens is an important economic trait, and the breeding of precocious and delayed puberty roosters is an important selection strategy for broilers. The comb serves as an important secondary sexual characteristic of roosters and determines their sexual precocity. Moreover, comb development is closely associated with gonad development in roosters. However, the underlying molecular mechanism regulating the sexual maturity of roosters has not yet been fully explored. Results In order to identify the genes related to precocious puberty in Qingyuan partridge roosters, and based on the synchrony of testis and combs development, combined with histological observation and RNA-seq method, the developmental status and gene expression profile of combs and testis were obtained. The results showed that during the early growth and development period (77 days of age), the development of combs and testis was significant in the high comb (H) group versus the low comb (L) group (p < 0.05); however, the morphological characteristic of the comb and testicular tissues converged during the late growth and development period (112 days of age) in the H and L groups. Based on these results, RNA-sequencing analysis was performed on the comb and testis tissues of the 77 and 112 days old Qingyuan Partridge roosters with different comb height traits. GO and KEGG analysis enrichment analysis showed that the differentially expressed genes were primarily enriched in MAPK signaling, VEGF signaling, and retinol metabolism pathways. Moreover, weighted correlation network analysis and module co-expression network analysis identified WNT6, AMH, IHH, STT3A, PEX16, KPNA7, CATHL2, ROR2, PAMR1, WISP2, IL17REL, NDRG4, CYP26B1, and CRHBP as the key genes associated with the regulation of precocity and delayed puberty in Qingyuan Partridge roosters. Conclusions In summary, we identified the key regulatory genes of sexual precocity in roosters, which provide a theoretical basis for understanding the developmental differences between precocious and delayed puberty in roosters.https://doi.org/10.1186/s12864-024-09960-2ChickenCombTestisSexual maturityWeighted correlation network analysis (WGCNA) |
| spellingShingle | Hao Qi Zhidan Deng Fei Ye Junwei Gou Miaoxin Huang Hai Xiang Hua Li Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages BMC Genomics Chicken Comb Testis Sexual maturity Weighted correlation network analysis (WGCNA) |
| title | Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages |
| title_full | Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages |
| title_fullStr | Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages |
| title_full_unstemmed | Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages |
| title_short | Analysis of the differentially expressed genes in the combs and testes of Qingyuan partridge roosters at different developmental stages |
| title_sort | analysis of the differentially expressed genes in the combs and testes of qingyuan partridge roosters at different developmental stages |
| topic | Chicken Comb Testis Sexual maturity Weighted correlation network analysis (WGCNA) |
| url | https://doi.org/10.1186/s12864-024-09960-2 |
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