Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.).
OsMADS16, a class B floral organ identity gene, plays a pivotal role in stamen formation in rice. To date, little is known about the interacting partners of OsMADS16 except for several MADS-box proteins. In this study, we constructed a high-quality cDNA library of young panicles (< 5 cm in length...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0221473 |
| _version_ | 1818588723324911616 |
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| author | Lan Kong Yuanlin Duan Yanfang Ye Zhengzheng Cai Feng Wang Xiaojie Qu Ronghua Qiu Chunyan Wu Weiren Wu |
| author_facet | Lan Kong Yuanlin Duan Yanfang Ye Zhengzheng Cai Feng Wang Xiaojie Qu Ronghua Qiu Chunyan Wu Weiren Wu |
| author_sort | Lan Kong |
| collection | DOAJ |
| description | OsMADS16, a class B floral organ identity gene, plays a pivotal role in stamen formation in rice. To date, little is known about the interacting partners of OsMADS16 except for several MADS-box proteins. In this study, we constructed a high-quality cDNA library of young panicles (< 5 cm in length) and performed yeast two-hybrid (Y2H) screening using OsMADS16 as bait. Eleven candidate proteins interacting with OsMADS16 were identified by Y2H and validated by BiFC and Co-IP assays. Subcellular localization results further confirmed the possibility of the interactions of OsMADS16 with 10 of the candidate proteins in natural rice cells. Bioinformatics analysis indicated that these partners exerted various molecular, cellular and physiological functions. Some of them were known or likely to be related to reproductive events, such as stamen primordium initiation, differentiation and development (OsMADS2, OsMADS4 and OsCOP9) and pollen development (OsbHLH40 and Os6PGDH). Our results provide an important reference for further research on OsMADS16-mediated regulation mechanism on floral organ development and pollen formation. |
| first_indexed | 2024-12-16T09:29:17Z |
| format | Article |
| id | doaj.art-964c8defbed941748d7e71c1d73a5e17 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T09:29:17Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-964c8defbed941748d7e71c1d73a5e172022-12-21T22:36:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01148e022147310.1371/journal.pone.0221473Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.).Lan KongYuanlin DuanYanfang YeZhengzheng CaiFeng WangXiaojie QuRonghua QiuChunyan WuWeiren WuOsMADS16, a class B floral organ identity gene, plays a pivotal role in stamen formation in rice. To date, little is known about the interacting partners of OsMADS16 except for several MADS-box proteins. In this study, we constructed a high-quality cDNA library of young panicles (< 5 cm in length) and performed yeast two-hybrid (Y2H) screening using OsMADS16 as bait. Eleven candidate proteins interacting with OsMADS16 were identified by Y2H and validated by BiFC and Co-IP assays. Subcellular localization results further confirmed the possibility of the interactions of OsMADS16 with 10 of the candidate proteins in natural rice cells. Bioinformatics analysis indicated that these partners exerted various molecular, cellular and physiological functions. Some of them were known or likely to be related to reproductive events, such as stamen primordium initiation, differentiation and development (OsMADS2, OsMADS4 and OsCOP9) and pollen development (OsbHLH40 and Os6PGDH). Our results provide an important reference for further research on OsMADS16-mediated regulation mechanism on floral organ development and pollen formation.https://doi.org/10.1371/journal.pone.0221473 |
| spellingShingle | Lan Kong Yuanlin Duan Yanfang Ye Zhengzheng Cai Feng Wang Xiaojie Qu Ronghua Qiu Chunyan Wu Weiren Wu Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). PLoS ONE |
| title | Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). |
| title_full | Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). |
| title_fullStr | Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). |
| title_full_unstemmed | Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). |
| title_short | Screening and analysis of proteins interacting with OsMADS16 in rice (Oryza sativa L.). |
| title_sort | screening and analysis of proteins interacting with osmads16 in rice oryza sativa l |
| url | https://doi.org/10.1371/journal.pone.0221473 |
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