Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas
Background/Aims: Obesity and diabetes (hereafter termed diabesity) are among the most challenging global health problems. Since the main pathophysiological complications in diabesity are hyperglycemia, hyperlipidemia, insulin resistance, cardiomyopathy, nephropathy, and urinary infections, the kidne...
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| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2013-12-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/356597 |
| _version_ | 1818508754564415488 |
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| author | Haider Raza Annie John Frank Christopher Howarth |
| author_facet | Haider Raza Annie John Frank Christopher Howarth |
| author_sort | Haider Raza |
| collection | DOAJ |
| description | Background/Aims: Obesity and diabetes (hereafter termed diabesity) are among the most challenging global health problems. Since the main pathophysiological complications in diabesity are hyperglycemia, hyperlipidemia, insulin resistance, cardiomyopathy, nephropathy, and urinary infections, the kidney and pancreas are the potential target organs affected in the above conditions. However, the precise molecular mechanisms of disease progression and complications are still unclear. The Zucker homozygous (FA/FA) diabetic fatty (ZDF) rat is a genetic model for obesity and type 2 diabetes. Our previous studies, using cardiac muscles have demonstrated metabolic and oxidative stress in ZDF rats. In the present study, our aim was to investigate oxidative stress associated metabolic complications in ZDF rat kidney and pancreas. Methods: Here we have measured oxidative stress, glutathione (GSH)-dependent metabolism and mitochondrial respiratory functions in the kidney and pancreas of ZDF and Zucker lean (ZL, +/FA) control rats. Results: Our results showed an increase in reactive oxygen species, NO production, lipid and protein peroxidation in ZDF rat kidney and pancreas accompanied by alterations in GSH-dependent metabolism and mitochondrial function. Western blot analysis has also confirmed increased expression of oxidative stress marker proteins in ZDF rats. Conclusion: We have demonstrated that ZDF rats develop metabolic complications associated with oxidative stress and mitochondrial dysfunction. Thus, these results might have implications in understanding the etiology and pathology of diabesity. |
| first_indexed | 2024-12-10T22:36:18Z |
| format | Article |
| id | doaj.art-96533ffa815a472188aafa7af7e8586c |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T22:36:18Z |
| publishDate | 2013-12-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-96533ffa815a472188aafa7af7e8586c2022-12-22T01:30:52ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-12-013261610162010.1159/000356597356597Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and PancreasHaider RazaAnnie JohnFrank Christopher HowarthBackground/Aims: Obesity and diabetes (hereafter termed diabesity) are among the most challenging global health problems. Since the main pathophysiological complications in diabesity are hyperglycemia, hyperlipidemia, insulin resistance, cardiomyopathy, nephropathy, and urinary infections, the kidney and pancreas are the potential target organs affected in the above conditions. However, the precise molecular mechanisms of disease progression and complications are still unclear. The Zucker homozygous (FA/FA) diabetic fatty (ZDF) rat is a genetic model for obesity and type 2 diabetes. Our previous studies, using cardiac muscles have demonstrated metabolic and oxidative stress in ZDF rats. In the present study, our aim was to investigate oxidative stress associated metabolic complications in ZDF rat kidney and pancreas. Methods: Here we have measured oxidative stress, glutathione (GSH)-dependent metabolism and mitochondrial respiratory functions in the kidney and pancreas of ZDF and Zucker lean (ZL, +/FA) control rats. Results: Our results showed an increase in reactive oxygen species, NO production, lipid and protein peroxidation in ZDF rat kidney and pancreas accompanied by alterations in GSH-dependent metabolism and mitochondrial function. Western blot analysis has also confirmed increased expression of oxidative stress marker proteins in ZDF rats. Conclusion: We have demonstrated that ZDF rats develop metabolic complications associated with oxidative stress and mitochondrial dysfunction. Thus, these results might have implications in understanding the etiology and pathology of diabesity.http://www.karger.com/Article/FullText/356597KidneyObesityZucker diabetic ratsPancreasOxidative stressMitochondrial dysfunction |
| spellingShingle | Haider Raza Annie John Frank Christopher Howarth Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas Cellular Physiology and Biochemistry Kidney Obesity Zucker diabetic rats Pancreas Oxidative stress Mitochondrial dysfunction |
| title | Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas |
| title_full | Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas |
| title_fullStr | Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas |
| title_full_unstemmed | Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas |
| title_short | Increased Metabolic Stress in Zucker Diabetic Fatty Rat Kidney and Pancreas |
| title_sort | increased metabolic stress in zucker diabetic fatty rat kidney and pancreas |
| topic | Kidney Obesity Zucker diabetic rats Pancreas Oxidative stress Mitochondrial dysfunction |
| url | http://www.karger.com/Article/FullText/356597 |
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