Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization
We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2019-04-01
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| Series: | The EuroBiotech Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/ebtj-2019-0011 |
| Summary: | We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification. |
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| ISSN: | 2564-615X |