Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization
We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2019-04-01
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| Series: | The EuroBiotech Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/ebtj-2019-0011 |
| _version_ | 1819117426560729088 |
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| author | Fatou Benoit Salzet Michel Fournier Isabelle |
| author_facet | Fatou Benoit Salzet Michel Fournier Isabelle |
| author_sort | Fatou Benoit |
| collection | DOAJ |
| description | We previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification. |
| first_indexed | 2024-12-22T05:32:48Z |
| format | Article |
| id | doaj.art-965579fe98bc488a82fca27301705bd8 |
| institution | Directory Open Access Journal |
| issn | 2564-615X |
| language | English |
| last_indexed | 2024-12-22T05:32:48Z |
| publishDate | 2019-04-01 |
| publisher | Sciendo |
| record_format | Article |
| series | The EuroBiotech Journal |
| spelling | doaj.art-965579fe98bc488a82fca27301705bd82022-12-21T18:37:25ZengSciendoThe EuroBiotech Journal2564-615X2019-04-01329710410.2478/ebtj-2019-0011ebtj-2019-0011Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/IonizationFatou Benoit0Salzet Michel1Fournier Isabelle2Université de Lille, Inserm U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59655Villeneuve d’Ascq Cedex, FranceUniversité de Lille, Inserm U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59655Villeneuve d’Ascq Cedex, FranceUniversité de Lille, Inserm U1192 - Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM), F-59655Villeneuve d’Ascq Cedex, FranceWe previously demonstrated that remote infrared Matrix Assisted Laser Desorption Ionization technology (Spidermass) using endogenous water as matrix (or so called water assisted laser desorption/ionization) was enabling real-time in vitro and in vivo analysis of clinical pathological tissues. In the present work, Spidermass was used to biotype human pathogens either from liquid bacteria growth in time course, from petri dish or on smears. Reproducibility experiments as well as bacteria dispersion and lipids identifications with SpiderMass in MS/MS mode were undertaken. The whole of the data establish that SpiderMass instrument allows real time bacteria biotyping and can be useful in clinic for pathogen identification.https://doi.org/10.2478/ebtj-2019-0011spidermassreal time analysismass spectrometrybacteria typing |
| spellingShingle | Fatou Benoit Salzet Michel Fournier Isabelle Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization The EuroBiotech Journal spidermass real time analysis mass spectrometry bacteria typing |
| title | Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization |
| title_full | Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization |
| title_fullStr | Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization |
| title_full_unstemmed | Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization |
| title_short | Real time human micro-organisms biotyping based on Water-Assisted Laser Desorption/Ionization |
| title_sort | real time human micro organisms biotyping based on water assisted laser desorption ionization |
| topic | spidermass real time analysis mass spectrometry bacteria typing |
| url | https://doi.org/10.2478/ebtj-2019-0011 |
| work_keys_str_mv | AT fatoubenoit realtimehumanmicroorganismsbiotypingbasedonwaterassistedlaserdesorptionionization AT salzetmichel realtimehumanmicroorganismsbiotypingbasedonwaterassistedlaserdesorptionionization AT fournierisabelle realtimehumanmicroorganismsbiotypingbasedonwaterassistedlaserdesorptionionization |