Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone
The soft and delicate tissue of the brain, which is the center of our coordination, is protected by its surrounding layers. The disruption of these layers results in complicated situations and serious health problems. The brain has three protective layers of bone or skull tissue, the blood tissue la...
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Razi Vaccine and Serum Research Institute
2021-10-01
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| Series: | Archives of Razi Institute |
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| Online Access: | https://archrazi.areeo.ac.ir/article_124838_7454ac88db7c5586e973d500ac6d1692.pdf |
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| author | A. Agarkova A. Tverskoi V. Morozov A. Tverskaya E. Morozova T. Mukhina |
| author_facet | A. Agarkova A. Tverskoi V. Morozov A. Tverskaya E. Morozova T. Mukhina |
| author_sort | A. Agarkova |
| collection | DOAJ |
| description | The soft and delicate tissue of the brain, which is the center of our coordination, is protected by its surrounding layers. The disruption of these layers results in complicated situations and serious health problems. The brain has three protective layers of bone or skull tissue, the blood tissue layer, and finally the meningeal layer. The layer of blood tissue contains the blood vessels that are located between the skull and the meningeal membranes. If germs or foreign matter enter the fluid through the blood vessels under any circumstances and cause infection, the bones that protect the meninges will break and cause tissue damage. The present study aimed to assess the histological and immunohistochemical characteristics of the brain of rats that underwent induced acute purulent pneumococcal meningitis after antibiotic therapy with Ceftriaxone. A number of 20 white adult male Wistar rats were assigned to three groups. The first group (n=5) regarded as the control were injected with a saline solution into the subarachnoid space in an equivalent amount. The second and third groups of rats (n=5 and 10, respectively) were infected with acute purulent meningitis by the injection of 10 μl of Streptococcus pneumoniae (S. pneumonia) suspension into the subarachnoid space of the brain using a 23-G needle. The various areas of the brains of rats after meningitis induced by S. pneumoniae were examined after the treatment with Ceftriaxone. The S. pneumoniae culture was injected into the subarachnoid space in the area of the rhomboid fossa. Treatment started 18 h after the injection. On day 10, a repeated puncture was performed with the analysis of cerebrospinal fluid in order to confirm the absence of meningitis; thereafter, the animals were taken out of the experiment. No signs of meningitis were found on histological examination. Mild perivascular and pericellular focal edema were revealed with signs of overload of the lymphatic system in the brain and focal ischemic changes in neurons. The investigation of expression with caspase-3 revealed a positive reaction of individual neurons. A positive reaction with antibodies to NeuN and Doublecortin was detected in most neurons; moreover, Glial fibrillary acidic protein (GFAP)-positive astrocytes and their processes were visualized in all layers of the brain substance. The reaction with neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP-2), CD 31, and CD 34 was negative. Typical structure and pictures pointed to an intact brain and purulent meningitis in the first and second groups. The microscopic image and the changes revealed during immunohistochemistry by dual corticosteroid antibodies and neuronal nuclear protein were characterized by predominantly cytoplasmic and perinuclear reactions, respectively. Some neurons are positive for caspase-3 and are related to changes in the characteristic of premature aging. |
| first_indexed | 2024-12-10T17:47:47Z |
| format | Article |
| id | doaj.art-96560e02234148adaf9e6ba73948d500 |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-12-10T17:47:47Z |
| publishDate | 2021-10-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-96560e02234148adaf9e6ba73948d5002022-12-22T01:39:11ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-10-017641005101210.22092/ari.2021.355885.1733124838Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with CeftriaxoneA. Agarkova0A. Tverskoi1V. Morozov2A. Tverskaya3E. Morozova4T. Mukhina5Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85Belgorod State University, 308015, Russia, Belgorod, Pobeda St. 85The soft and delicate tissue of the brain, which is the center of our coordination, is protected by its surrounding layers. The disruption of these layers results in complicated situations and serious health problems. The brain has three protective layers of bone or skull tissue, the blood tissue layer, and finally the meningeal layer. The layer of blood tissue contains the blood vessels that are located between the skull and the meningeal membranes. If germs or foreign matter enter the fluid through the blood vessels under any circumstances and cause infection, the bones that protect the meninges will break and cause tissue damage. The present study aimed to assess the histological and immunohistochemical characteristics of the brain of rats that underwent induced acute purulent pneumococcal meningitis after antibiotic therapy with Ceftriaxone. A number of 20 white adult male Wistar rats were assigned to three groups. The first group (n=5) regarded as the control were injected with a saline solution into the subarachnoid space in an equivalent amount. The second and third groups of rats (n=5 and 10, respectively) were infected with acute purulent meningitis by the injection of 10 μl of Streptococcus pneumoniae (S. pneumonia) suspension into the subarachnoid space of the brain using a 23-G needle. The various areas of the brains of rats after meningitis induced by S. pneumoniae were examined after the treatment with Ceftriaxone. The S. pneumoniae culture was injected into the subarachnoid space in the area of the rhomboid fossa. Treatment started 18 h after the injection. On day 10, a repeated puncture was performed with the analysis of cerebrospinal fluid in order to confirm the absence of meningitis; thereafter, the animals were taken out of the experiment. No signs of meningitis were found on histological examination. Mild perivascular and pericellular focal edema were revealed with signs of overload of the lymphatic system in the brain and focal ischemic changes in neurons. The investigation of expression with caspase-3 revealed a positive reaction of individual neurons. A positive reaction with antibodies to NeuN and Doublecortin was detected in most neurons; moreover, Glial fibrillary acidic protein (GFAP)-positive astrocytes and their processes were visualized in all layers of the brain substance. The reaction with neuron-specific enolase (NSE), microtubule-associated protein 2 (MAP-2), CD 31, and CD 34 was negative. Typical structure and pictures pointed to an intact brain and purulent meningitis in the first and second groups. The microscopic image and the changes revealed during immunohistochemistry by dual corticosteroid antibodies and neuronal nuclear protein were characterized by predominantly cytoplasmic and perinuclear reactions, respectively. Some neurons are positive for caspase-3 and are related to changes in the characteristic of premature aging.https://archrazi.areeo.ac.ir/article_124838_7454ac88db7c5586e973d500ac6d1692.pdfacute meningitisceftriaxoneimmunohistochemical markersbrain of rats |
| spellingShingle | A. Agarkova A. Tverskoi V. Morozov A. Tverskaya E. Morozova T. Mukhina Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone Archives of Razi Institute acute meningitis ceftriaxone immunohistochemical markers brain of rats |
| title | Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone |
| title_full | Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone |
| title_fullStr | Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone |
| title_full_unstemmed | Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone |
| title_short | Evaluation of Rat Brain Morphology Following the Induction of Acute Meningitis Treated with Ceftriaxone |
| title_sort | evaluation of rat brain morphology following the induction of acute meningitis treated with ceftriaxone |
| topic | acute meningitis ceftriaxone immunohistochemical markers brain of rats |
| url | https://archrazi.areeo.ac.ir/article_124838_7454ac88db7c5586e973d500ac6d1692.pdf |
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