The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds
The antioxidant activity of food compounds is one of the properties generating the most interest, due to its health benefits and correlation with the prevention of chronic disease. This activity is usually measured using in vitro assays, which cannot predict in vivo effects or mechanisms of action....
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/19/5786 |
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| author | Cristina Arteaga Nuria Boix Elisabet Teixido Fernanda Marizande Santiago Cadena Alberto Bustillos |
| author_facet | Cristina Arteaga Nuria Boix Elisabet Teixido Fernanda Marizande Santiago Cadena Alberto Bustillos |
| author_sort | Cristina Arteaga |
| collection | DOAJ |
| description | The antioxidant activity of food compounds is one of the properties generating the most interest, due to its health benefits and correlation with the prevention of chronic disease. This activity is usually measured using in vitro assays, which cannot predict in vivo effects or mechanisms of action. The objective of this study was to evaluate the in vivo protective effects of six phenolic compounds (naringenin, apigenin, rutin, oleuropein, chlorogenic acid, and curcumin) and three carotenoids (lycopene B, β-carotene, and astaxanthin) naturally present in foods using a zebrafish embryo model. The zebrafish embryo was pretreated with each of the nine antioxidant compounds and then exposed to tert-butyl hydroperoxide (tBOOH), a known inducer of oxidative stress in zebrafish. Significant differences were determined by comparing the concentration-response of the tBOOH induced lethality and dysmorphogenesis against the pretreated embryos with the antioxidant compounds. A protective effect of each compound, except β-carotene, against oxidative-stress-induced lethality was found. Furthermore, apigenin, rutin, and curcumin also showed protective effects against dysmorphogenesis. On the other hand, β-carotene exhibited increased lethality and dysmorphogenesis compared to the tBOOH treatment alone. |
| first_indexed | 2024-03-10T06:55:09Z |
| format | Article |
| id | doaj.art-965bd3e628b84568b64be741f270ab8f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T06:55:09Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-965bd3e628b84568b64be741f270ab8f2023-11-22T16:32:36ZengMDPI AGMolecules1420-30492021-09-012619578610.3390/molecules26195786The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant CompoundsCristina Arteaga0Nuria Boix1Elisabet Teixido2Fernanda Marizande3Santiago Cadena4Alberto Bustillos5Toxicology Unit, Pharmacology, Toxicology and Therapeutical Chemistry Department, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, SpainToxicology Unit, Pharmacology, Toxicology and Therapeutical Chemistry Department, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, SpainToxicology Unit, Pharmacology, Toxicology and Therapeutical Chemistry Department, Pharmacy School, University of Barcelona, Avda Joan XXIII s/n, 08028 Barcelona, SpainFaculty of Health Sciences, Nutrition and Dietetics, Technical University of Ambato, Ambato 180207, EcuadorFaculty of Applied Sciences, International SEK University, Quito 170134, EcuadorFaculty of Health Sciences, Medicine, Technical University of Ambato, Ambato 180207, EcuadorThe antioxidant activity of food compounds is one of the properties generating the most interest, due to its health benefits and correlation with the prevention of chronic disease. This activity is usually measured using in vitro assays, which cannot predict in vivo effects or mechanisms of action. The objective of this study was to evaluate the in vivo protective effects of six phenolic compounds (naringenin, apigenin, rutin, oleuropein, chlorogenic acid, and curcumin) and three carotenoids (lycopene B, β-carotene, and astaxanthin) naturally present in foods using a zebrafish embryo model. The zebrafish embryo was pretreated with each of the nine antioxidant compounds and then exposed to tert-butyl hydroperoxide (tBOOH), a known inducer of oxidative stress in zebrafish. Significant differences were determined by comparing the concentration-response of the tBOOH induced lethality and dysmorphogenesis against the pretreated embryos with the antioxidant compounds. A protective effect of each compound, except β-carotene, against oxidative-stress-induced lethality was found. Furthermore, apigenin, rutin, and curcumin also showed protective effects against dysmorphogenesis. On the other hand, β-carotene exhibited increased lethality and dysmorphogenesis compared to the tBOOH treatment alone.https://www.mdpi.com/1420-3049/26/19/5786oxidative stresszebrafish embryoantioxidant effectpolyphenolscarotenoids |
| spellingShingle | Cristina Arteaga Nuria Boix Elisabet Teixido Fernanda Marizande Santiago Cadena Alberto Bustillos The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds Molecules oxidative stress zebrafish embryo antioxidant effect polyphenols carotenoids |
| title | The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds |
| title_full | The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds |
| title_fullStr | The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds |
| title_full_unstemmed | The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds |
| title_short | The Zebrafish Embryo as a Model to Test Protective Effects of Food Antioxidant Compounds |
| title_sort | zebrafish embryo as a model to test protective effects of food antioxidant compounds |
| topic | oxidative stress zebrafish embryo antioxidant effect polyphenols carotenoids |
| url | https://www.mdpi.com/1420-3049/26/19/5786 |
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