External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa.
During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global pre...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC5426792?pdf=render |
| _version_ | 1819212465521557504 |
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| author | Heinz Ellerbrok Sonja Jacobsen Pranav Patel Toni Rieger Markus Eickmann Stephan Becker Stephan Günther Dhamari Naidoo Livia Schrick Kathrin Keeren Angelina Targosz Anette Teichmann Pierre Formenty Matthias Niedrig |
| author_facet | Heinz Ellerbrok Sonja Jacobsen Pranav Patel Toni Rieger Markus Eickmann Stephan Becker Stephan Günther Dhamari Naidoo Livia Schrick Kathrin Keeren Angelina Targosz Anette Teichmann Pierre Formenty Matthias Niedrig |
| author_sort | Heinz Ellerbrok |
| collection | DOAJ |
| description | During the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola diagnostics. Detection of viral RNA by reverse transcription polymerase chain reaction has proven effective for diagnosis of ebolavirus disease and several assays are available. However, reliability of these assays is largely unknown and requires serious evaluation. Therefore, a proficiency test panel of 11 samples was generated and distributed on a global scale. Panels were analyzed by 83 expert laboratories and 106 data sets were returned. From these 78 results were rated optimal and 3 acceptable, 25 indicated need for improvement. While performance of the laboratories deployed to West Africa was superior to the overall performance there was no significant difference between the different assays applied. |
| first_indexed | 2024-12-23T06:43:24Z |
| format | Article |
| id | doaj.art-965c0c138d344363a93d5d714ed4e3e8 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-23T06:43:24Z |
| publishDate | 2017-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-965c0c138d344363a93d5d714ed4e3e82022-12-21T17:56:38ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352017-05-01115e000557010.1371/journal.pntd.0005570External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa.Heinz EllerbrokSonja JacobsenPranav PatelToni RiegerMarkus EickmannStephan BeckerStephan GüntherDhamari NaidooLivia SchrickKathrin KeerenAngelina TargoszAnette TeichmannPierre FormentyMatthias NiedrigDuring the recent Ebola outbreak in West Africa several international mobile laboratories were deployed to the mainly affected countries Guinea, Sierra Leone and Liberia to provide ebolavirus diagnostic capacity. Additionally, imported cases and small outbreaks in other countries required global preparedness for Ebola diagnostics. Detection of viral RNA by reverse transcription polymerase chain reaction has proven effective for diagnosis of ebolavirus disease and several assays are available. However, reliability of these assays is largely unknown and requires serious evaluation. Therefore, a proficiency test panel of 11 samples was generated and distributed on a global scale. Panels were analyzed by 83 expert laboratories and 106 data sets were returned. From these 78 results were rated optimal and 3 acceptable, 25 indicated need for improvement. While performance of the laboratories deployed to West Africa was superior to the overall performance there was no significant difference between the different assays applied.http://europepmc.org/articles/PMC5426792?pdf=render |
| spellingShingle | Heinz Ellerbrok Sonja Jacobsen Pranav Patel Toni Rieger Markus Eickmann Stephan Becker Stephan Günther Dhamari Naidoo Livia Schrick Kathrin Keeren Angelina Targosz Anette Teichmann Pierre Formenty Matthias Niedrig External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. PLoS Neglected Tropical Diseases |
| title | External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. |
| title_full | External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. |
| title_fullStr | External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. |
| title_full_unstemmed | External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. |
| title_short | External quality assessment study for ebolavirus PCR-diagnostic promotes international preparedness during the 2014 - 2016 Ebola outbreak in West Africa. |
| title_sort | external quality assessment study for ebolavirus pcr diagnostic promotes international preparedness during the 2014 2016 ebola outbreak in west africa |
| url | http://europepmc.org/articles/PMC5426792?pdf=render |
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