A Proteomic Study of the Bioactivity of <i>Annona muricata</i> Leaf Extracts in HT-1080 Fibrosarcoma Cells
Graviola (<i>Annona muricata</i>) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from <i>Annona muricata</i> leaves recently allowed us to identify dozens of bioactive compounds...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/15/12021 |
| Summary: | Graviola (<i>Annona muricata</i>) is a tropical plant with many traditional ethnobotanic uses and pharmacologic applications. A metabolomic study of both aqueous and DMSO extracts from <i>Annona muricata</i> leaves recently allowed us to identify dozens of bioactive compounds. In the present study, we use a proteomic approach to detect altered patterns in proteins on both conditioned media and extracts of HT-1080 fibrosarcoma cells under treatment conditions, revealing new potential bioactivities of <i>Annona muricata</i> extracts. Our results reveal the complete sets of deregulated proteins after treatment with aqueous and DMSO extracts from <i>Annona muricata</i> leaves. Functional enrichment analysis of proteomic data suggests deregulation of cell cycle and iron metabolism, which are experimentally validated in vitro. Additional experimental data reveal that DMSO extracts protect HT-1080 fibrosarcoma cells and HMEC-1 endothelial cells from ferroptosis. Data from our proteomic study are available via ProteomeXchange with identifier PXD042354. |
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| ISSN: | 1661-6596 1422-0067 |