Western blotting (immunoblotting): history, theory, uses, protocol and problems
Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues. The history surrounding the origin of western blotting, the theory behind the western b...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Future Science Ltd
2023-09-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/btn-2022-0034 |
| _version_ | 1797639363838869504 |
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| author | Rasheed Sule Gabriela Rivera Aldrin V Gomes |
| author_facet | Rasheed Sule Gabriela Rivera Aldrin V Gomes |
| author_sort | Rasheed Sule |
| collection | DOAJ |
| description | Western blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues. The history surrounding the origin of western blotting, the theory behind the western blotting technique, a comprehensive protocol and the uses of western blotting are presented. Lesser known and significant problems in the western blotting field and troubleshooting of common problems are highlighted and discussed. This work is a comprehensive primer and guide for new western blotting researchers and those interested in a better understanding of the technique or getting better results. |
| first_indexed | 2024-03-11T13:16:42Z |
| format | Article |
| id | doaj.art-965df2efa35143418c6ae3bc9261107e |
| institution | Directory Open Access Journal |
| issn | 0736-6205 1940-9818 |
| language | English |
| last_indexed | 2024-03-11T13:16:42Z |
| publishDate | 2023-09-01 |
| publisher | Future Science Ltd |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj.art-965df2efa35143418c6ae3bc9261107e2023-11-03T12:07:30ZengFuture Science LtdBioTechniques0736-62051940-98182023-09-017539911410.2144/btn-2022-0034Western blotting (immunoblotting): history, theory, uses, protocol and problemsRasheed Sule0Gabriela Rivera1Aldrin V Gomes21Department of Neurobiology, Physiology & Behavior, University of California, Davis, Davis, CA 95616, USA1Department of Neurobiology, Physiology & Behavior, University of California, Davis, Davis, CA 95616, USA1Department of Neurobiology, Physiology & Behavior, University of California, Davis, Davis, CA 95616, USAWestern blotting (immunoblotting) is a powerful and commonly used technique that is capable of detecting or semiquantifying an individual protein from complex mixtures of proteins extracted from cells or tissues. The history surrounding the origin of western blotting, the theory behind the western blotting technique, a comprehensive protocol and the uses of western blotting are presented. Lesser known and significant problems in the western blotting field and troubleshooting of common problems are highlighted and discussed. This work is a comprehensive primer and guide for new western blotting researchers and those interested in a better understanding of the technique or getting better results.https://www.future-science.com/doi/10.2144/btn-2022-0034antibodiesgel electrophoresisimmunoblottingproblemsprotocolsample preparation |
| spellingShingle | Rasheed Sule Gabriela Rivera Aldrin V Gomes Western blotting (immunoblotting): history, theory, uses, protocol and problems BioTechniques antibodies gel electrophoresis immunoblotting problems protocol sample preparation |
| title | Western blotting (immunoblotting): history, theory, uses, protocol and problems |
| title_full | Western blotting (immunoblotting): history, theory, uses, protocol and problems |
| title_fullStr | Western blotting (immunoblotting): history, theory, uses, protocol and problems |
| title_full_unstemmed | Western blotting (immunoblotting): history, theory, uses, protocol and problems |
| title_short | Western blotting (immunoblotting): history, theory, uses, protocol and problems |
| title_sort | western blotting immunoblotting history theory uses protocol and problems |
| topic | antibodies gel electrophoresis immunoblotting problems protocol sample preparation |
| url | https://www.future-science.com/doi/10.2144/btn-2022-0034 |
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