PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
The prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inh...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-12-01
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| Series: | Journal of Nutrition & Intermediary Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352385917302682 |
| _version_ | 1811298106891829248 |
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| author | Muni Swamy Ganjayi Balaji Meriga Balaji Hari Lokanatha Oruganti Sreenivasulu Dasari Ramgopal Mopuri |
| author_facet | Muni Swamy Ganjayi Balaji Meriga Balaji Hari Lokanatha Oruganti Sreenivasulu Dasari Ramgopal Mopuri |
| author_sort | Muni Swamy Ganjayi |
| collection | DOAJ |
| description | The prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inhibit adipogenesis, pancreatic lipase and amylase activity. The maximum inhibitory activity of lipase (78%) and amylase (81%) was noticed at 250 μg/mL of TPPF. During cytotoxicity assay with MTT, TPPF did not show any cytotoxic effect on 3T3-L1 cells up to a concentration of 400 μg/mL. Oil Red O staining of 3T3-L1 cells showed considerable reduction in adipocytes differentiation and lipid accumulation in the presence of TPPF (250 μg/mL) when compared with untreated 3T3-L1 cells. HPLC analysis of TPPF revealed the presence of Gallic acid, Ellagic acid and Quercetin as the major components. Our results suggest that, TPPF may be useful in attenuating obesity ailments. Keywords: Adipogenesis, α-Amylase, Pancreatic lipase, Terminalia paniculata and 3T3L1cells |
| first_indexed | 2024-04-13T06:14:44Z |
| format | Article |
| id | doaj.art-965e901eca9543e695d51e0c27481111 |
| institution | Directory Open Access Journal |
| issn | 2352-3859 |
| language | English |
| last_indexed | 2024-04-13T06:14:44Z |
| publishDate | 2017-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Nutrition & Intermediary Metabolism |
| spelling | doaj.art-965e901eca9543e695d51e0c274811112022-12-22T02:58:52ZengElsevierJournal of Nutrition & Intermediary Metabolism2352-38592017-12-01101925PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiationMuni Swamy Ganjayi0Balaji Meriga1Balaji Hari2Lokanatha Oruganti3Sreenivasulu Dasari4Ramgopal Mopuri5Department of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 India; Corresponding author.Department of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biotechnology, Sri Krishnadevaraya University, Anantapur, AP, IndiaThe prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inhibit adipogenesis, pancreatic lipase and amylase activity. The maximum inhibitory activity of lipase (78%) and amylase (81%) was noticed at 250 μg/mL of TPPF. During cytotoxicity assay with MTT, TPPF did not show any cytotoxic effect on 3T3-L1 cells up to a concentration of 400 μg/mL. Oil Red O staining of 3T3-L1 cells showed considerable reduction in adipocytes differentiation and lipid accumulation in the presence of TPPF (250 μg/mL) when compared with untreated 3T3-L1 cells. HPLC analysis of TPPF revealed the presence of Gallic acid, Ellagic acid and Quercetin as the major components. Our results suggest that, TPPF may be useful in attenuating obesity ailments. Keywords: Adipogenesis, α-Amylase, Pancreatic lipase, Terminalia paniculata and 3T3L1cellshttp://www.sciencedirect.com/science/article/pii/S2352385917302682 |
| spellingShingle | Muni Swamy Ganjayi Balaji Meriga Balaji Hari Lokanatha Oruganti Sreenivasulu Dasari Ramgopal Mopuri PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation Journal of Nutrition & Intermediary Metabolism |
| title | PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation |
| title_full | PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation |
| title_fullStr | PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation |
| title_full_unstemmed | PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation |
| title_short | PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation |
| title_sort | polyphenolic rich fraction of terminalia paniculata attenuates obesity through inhibition of pancreatic amylase lipase and 3t3 l1 adipocyte differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2352385917302682 |
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