A Comparison of the Costs and Benefits of Bacterial Gene Expression.
To study how a bacterium allocates its resources, we compared the costs and benefits of most (86%) of the proteins in Escherichia coli K-12 during growth in minimal glucose medium. The cost or investment in each protein was estimated from ribosomal profiling data, and the benefit of each protein was...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5053530?pdf=render |
| _version_ | 1818859120679190528 |
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| author | Morgan N Price Kelly M Wetmore Adam M Deutschbauer Adam P Arkin |
| author_facet | Morgan N Price Kelly M Wetmore Adam M Deutschbauer Adam P Arkin |
| author_sort | Morgan N Price |
| collection | DOAJ |
| description | To study how a bacterium allocates its resources, we compared the costs and benefits of most (86%) of the proteins in Escherichia coli K-12 during growth in minimal glucose medium. The cost or investment in each protein was estimated from ribosomal profiling data, and the benefit of each protein was measured by assaying a library of transposon mutants. We found that proteins that are important for fitness are usually highly expressed, and 95% of these proteins are expressed at above 13 parts per million (ppm). Conversely, proteins that do not measurably benefit the host (with a benefit of less than 5% per generation) tend to be weakly expressed, with a median expression of 13 ppm. In aggregate, genes with no detectable benefit account for 31% of protein production, or about 22% if we correct for genetic redundancy. Although some of the apparently unnecessary expression could have subtle benefits in minimal glucose medium, the majority of the burden is due to genes that are important in other conditions. We propose that at least 13% of the cell's protein is "on standby" in case conditions change. |
| first_indexed | 2024-12-19T09:07:08Z |
| format | Article |
| id | doaj.art-965f218823d8447fa3963c081857e6fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T09:07:08Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-965f218823d8447fa3963c081857e6fc2022-12-21T20:28:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016431410.1371/journal.pone.0164314A Comparison of the Costs and Benefits of Bacterial Gene Expression.Morgan N PriceKelly M WetmoreAdam M DeutschbauerAdam P ArkinTo study how a bacterium allocates its resources, we compared the costs and benefits of most (86%) of the proteins in Escherichia coli K-12 during growth in minimal glucose medium. The cost or investment in each protein was estimated from ribosomal profiling data, and the benefit of each protein was measured by assaying a library of transposon mutants. We found that proteins that are important for fitness are usually highly expressed, and 95% of these proteins are expressed at above 13 parts per million (ppm). Conversely, proteins that do not measurably benefit the host (with a benefit of less than 5% per generation) tend to be weakly expressed, with a median expression of 13 ppm. In aggregate, genes with no detectable benefit account for 31% of protein production, or about 22% if we correct for genetic redundancy. Although some of the apparently unnecessary expression could have subtle benefits in minimal glucose medium, the majority of the burden is due to genes that are important in other conditions. We propose that at least 13% of the cell's protein is "on standby" in case conditions change.http://europepmc.org/articles/PMC5053530?pdf=render |
| spellingShingle | Morgan N Price Kelly M Wetmore Adam M Deutschbauer Adam P Arkin A Comparison of the Costs and Benefits of Bacterial Gene Expression. PLoS ONE |
| title | A Comparison of the Costs and Benefits of Bacterial Gene Expression. |
| title_full | A Comparison of the Costs and Benefits of Bacterial Gene Expression. |
| title_fullStr | A Comparison of the Costs and Benefits of Bacterial Gene Expression. |
| title_full_unstemmed | A Comparison of the Costs and Benefits of Bacterial Gene Expression. |
| title_short | A Comparison of the Costs and Benefits of Bacterial Gene Expression. |
| title_sort | comparison of the costs and benefits of bacterial gene expression |
| url | http://europepmc.org/articles/PMC5053530?pdf=render |
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