‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>)
Recently, environmentally friendly crop improvements using next-generation plant biostimulants (PBs) come to the forefront in agriculture, regardless of whether they are used by scientists, farmers, or industries. Various organic and inorganic solutions have been investigated by researchers and prod...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/4/743 |
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| author | Barbara Kutasy Márta Kiniczky Kincső Decsi Nikoletta Kálmán Géza Hegedűs Zoltán Péter Alföldi Eszter Virág |
| author_facet | Barbara Kutasy Márta Kiniczky Kincső Decsi Nikoletta Kálmán Géza Hegedűs Zoltán Péter Alföldi Eszter Virág |
| author_sort | Barbara Kutasy |
| collection | DOAJ |
| description | Recently, environmentally friendly crop improvements using next-generation plant biostimulants (PBs) come to the forefront in agriculture, regardless of whether they are used by scientists, farmers, or industries. Various organic and inorganic solutions have been investigated by researchers and producers, focusing on tolerance to abiotic and biotic stresses, crop quality, or nutritional deficiency. Garlic has been considered a universal remedy ever since antiquity. A supercritical carbon dioxide garlic extract encapsulated in nanoscale liposomes composed of plant-derived lipids was examined as a possible PB agent. The present study focused on the characterization of the genes associated with the pathways involved in defense response triggered by the liposome nanoparticles that were loaded with supercritical garlic extracts. This material was applied to <i>Triticum aestivum</i> in greenhouse experiments using foliar spraying. The effects were examined in a large-scale genome-wide transcriptional profiling experiment by collecting the samples four times (0 min, used as a control, and 15 min, 24 h, and 48 h after spraying). Based on a time-course expression analysis, the dynamics of the cellular response were determined by examining differentially expressed genes and applying a cluster analysis. The results suggested an enhanced expression of abscisic acid (ABA) pathway and pathogenesis-related (PR) genes, of which positive regulation was found for the AP2-, C2H2-, HD-ZIP-, and MYB-related transcription factor families. |
| first_indexed | 2024-03-11T08:15:58Z |
| format | Article |
| id | doaj.art-965fe07063004a31a20aa0a9806f5db9 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-11T08:15:58Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-965fe07063004a31a20aa0a9806f5db92023-11-16T22:46:52ZengMDPI AGPlants2223-77472023-02-0112474310.3390/plants12040743‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>)Barbara Kutasy0Márta Kiniczky1Kincső Decsi2Nikoletta Kálmán3Géza Hegedűs4Zoltán Péter Alföldi5Eszter Virág6Department of Plant Physiology and Plant Ecology, Georgikon Campus, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Festetics Str. 7, 8360 Keszthely, HungaryResearch Institute for Medicinal Plants and Herbs Ltd., Lupaszigeti Str. 4, 2011 Budakalász, HungaryDepartment of Plant Physiology and Plant Ecology, Georgikon Campus, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Festetics Str. 7, 8360 Keszthely, HungaryDepartment of Biochemistry and Medical Chemistry, University of Pécs Medical School, Szigeti Str. 12, 7633 Pécs, HungaryDepartment of Information Technology and Its Applications, Faculty of Information Technology, University of Pannonia, Gasparich Str. 18, 8900 Zalaegerszeg, HungaryDepartment of Environmental Biology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences, Festetics Str. 7, 8360 Keszthely, HungaryResearch Institute for Medicinal Plants and Herbs Ltd., Lupaszigeti Str. 4, 2011 Budakalász, HungaryRecently, environmentally friendly crop improvements using next-generation plant biostimulants (PBs) come to the forefront in agriculture, regardless of whether they are used by scientists, farmers, or industries. Various organic and inorganic solutions have been investigated by researchers and producers, focusing on tolerance to abiotic and biotic stresses, crop quality, or nutritional deficiency. Garlic has been considered a universal remedy ever since antiquity. A supercritical carbon dioxide garlic extract encapsulated in nanoscale liposomes composed of plant-derived lipids was examined as a possible PB agent. The present study focused on the characterization of the genes associated with the pathways involved in defense response triggered by the liposome nanoparticles that were loaded with supercritical garlic extracts. This material was applied to <i>Triticum aestivum</i> in greenhouse experiments using foliar spraying. The effects were examined in a large-scale genome-wide transcriptional profiling experiment by collecting the samples four times (0 min, used as a control, and 15 min, 24 h, and 48 h after spraying). Based on a time-course expression analysis, the dynamics of the cellular response were determined by examining differentially expressed genes and applying a cluster analysis. The results suggested an enhanced expression of abscisic acid (ABA) pathway and pathogenesis-related (PR) genes, of which positive regulation was found for the AP2-, C2H2-, HD-ZIP-, and MYB-related transcription factor families.https://www.mdpi.com/2223-7747/12/4/743Illumina sequencing<i>Allium sativum</i>genome-wide transcriptional profilingbiostimulantgarlicabscisic acid |
| spellingShingle | Barbara Kutasy Márta Kiniczky Kincső Decsi Nikoletta Kálmán Géza Hegedűs Zoltán Péter Alföldi Eszter Virág ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) Plants Illumina sequencing <i>Allium sativum</i> genome-wide transcriptional profiling biostimulant garlic abscisic acid |
| title | ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) |
| title_full | ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) |
| title_fullStr | ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) |
| title_full_unstemmed | ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) |
| title_short | ‘Garlic-lipo’4Plants: Liposome-Encapsulated Garlic Extract Stimulates ABA Pathway and PR Genes in Wheat (<i>Triticum aestivum</i>) |
| title_sort | garlic lipo 4plants liposome encapsulated garlic extract stimulates aba pathway and pr genes in wheat i triticum aestivum i |
| topic | Illumina sequencing <i>Allium sativum</i> genome-wide transcriptional profiling biostimulant garlic abscisic acid |
| url | https://www.mdpi.com/2223-7747/12/4/743 |
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