Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells
Many genomic, anatomical and functional differences exist between the medullary (MTAL) and the cortical thick ascending limb of the loop of Henle (CTAL), including a higher expression of claudin-10 (CLDN10) in the MTAL than in the CTAL. Therefore, we assessed to what extent the <i>Cldn10</i...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-04-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/7/4008 |
| _version_ | 1797212442991788032 |
|---|---|
| author | Gaelle Brideau Lydie Cheval Camille Griveau Wung-Man Evelyne Ling Loïc Lievre Gilles Crambert Dominik Müller Jovana Broćić Emeline Cherchame Pascal Houillier Caroline Prot-Bertoye |
| author_facet | Gaelle Brideau Lydie Cheval Camille Griveau Wung-Man Evelyne Ling Loïc Lievre Gilles Crambert Dominik Müller Jovana Broćić Emeline Cherchame Pascal Houillier Caroline Prot-Bertoye |
| author_sort | Gaelle Brideau |
| collection | DOAJ |
| description | Many genomic, anatomical and functional differences exist between the medullary (MTAL) and the cortical thick ascending limb of the loop of Henle (CTAL), including a higher expression of claudin-10 (CLDN10) in the MTAL than in the CTAL. Therefore, we assessed to what extent the <i>Cldn10</i> gene expression is a determinant of differential gene expression between MTAL and CTAL. RNAs extracted from CTAL and MTAL microdissected from wild type (WT) and Cldn10 knock out mice (cKO) were analyzed by RNAseq. Differential and enrichment analyses (GSEA) were performed with interactive R Shiny software. Between WT and cKO MTAL, 637 genes were differentially expressed, whereas only 76 were differentially expressed between WT and cKO CTAL. Gene expression patterns and GSEA analyses in all replicates showed that WT MTAL did not cluster with the other replicates; no hierarchical clustering could be found between WT CTAL, cKO CTAL and cKO MTAL. Compared to WT replicates, cKO replicates were enriched in <i>Cldn16</i>, <i>Cldn19</i>, <i>Pth1r</i>, (parathyroid hormone receptor type 1), <i>Casr</i> (calcium sensing receptor) and <i>Vdr</i> (Vitamin D Receptor) mRNA in both the cortex and medulla. <i>Cldn10</i> is associated with gene expression patterns, including genes specifically involved in divalent cations reabsorption in the TAL. |
| first_indexed | 2024-04-24T10:42:27Z |
| format | Article |
| id | doaj.art-9669816d3ebe4569b2749162199d4f9e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T10:42:27Z |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-9669816d3ebe4569b2749162199d4f9e2024-04-12T13:20:23ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-04-01257400810.3390/ijms25074008Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb CellsGaelle Brideau0Lydie Cheval1Camille Griveau2Wung-Man Evelyne Ling3Loïc Lievre4Gilles Crambert5Dominik Müller6Jovana Broćić7Emeline Cherchame8Pascal Houillier9Caroline Prot-Bertoye10Centre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceDepartment of Pediatrics, Division of Gastroenterology, Nephrology and Metabolic Diseases, Charité-Universitätsmedizin Berlin, DE-13353 Berlin, GermanyParis Brain Institute (ICM), Hôpital Pitié-Salpêtrière, Inserm U 1127, CNRS UMR 7225, Sorbonne Université, Data Analysis Core Platform, F-75013 Paris, FranceParis Brain Institute (ICM), Hôpital Pitié-Salpêtrière, Inserm U 1127, CNRS UMR 7225, Sorbonne Université, Data Analysis Core Platform, F-75013 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceCentre de Recherche des Cordeliers, Institut National de la Santé et de la Recherche Médicale, Sorbonne Université, Université Paris Cité, F-75006 Paris, FranceMany genomic, anatomical and functional differences exist between the medullary (MTAL) and the cortical thick ascending limb of the loop of Henle (CTAL), including a higher expression of claudin-10 (CLDN10) in the MTAL than in the CTAL. Therefore, we assessed to what extent the <i>Cldn10</i> gene expression is a determinant of differential gene expression between MTAL and CTAL. RNAs extracted from CTAL and MTAL microdissected from wild type (WT) and Cldn10 knock out mice (cKO) were analyzed by RNAseq. Differential and enrichment analyses (GSEA) were performed with interactive R Shiny software. Between WT and cKO MTAL, 637 genes were differentially expressed, whereas only 76 were differentially expressed between WT and cKO CTAL. Gene expression patterns and GSEA analyses in all replicates showed that WT MTAL did not cluster with the other replicates; no hierarchical clustering could be found between WT CTAL, cKO CTAL and cKO MTAL. Compared to WT replicates, cKO replicates were enriched in <i>Cldn16</i>, <i>Cldn19</i>, <i>Pth1r</i>, (parathyroid hormone receptor type 1), <i>Casr</i> (calcium sensing receptor) and <i>Vdr</i> (Vitamin D Receptor) mRNA in both the cortex and medulla. <i>Cldn10</i> is associated with gene expression patterns, including genes specifically involved in divalent cations reabsorption in the TAL.https://www.mdpi.com/1422-0067/25/7/4008tight junctionepitheliumkidneyclaudin-10HELIX syndromethick ascending limb of the loop of Henle |
| spellingShingle | Gaelle Brideau Lydie Cheval Camille Griveau Wung-Man Evelyne Ling Loïc Lievre Gilles Crambert Dominik Müller Jovana Broćić Emeline Cherchame Pascal Houillier Caroline Prot-Bertoye Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells International Journal of Molecular Sciences tight junction epithelium kidney claudin-10 HELIX syndrome thick ascending limb of the loop of Henle |
| title | Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells |
| title_full | Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells |
| title_fullStr | Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells |
| title_full_unstemmed | Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells |
| title_short | Claudin-10 Expression and the Gene Expression Pattern of Thick Ascending Limb Cells |
| title_sort | claudin 10 expression and the gene expression pattern of thick ascending limb cells |
| topic | tight junction epithelium kidney claudin-10 HELIX syndrome thick ascending limb of the loop of Henle |
| url | https://www.mdpi.com/1422-0067/25/7/4008 |
| work_keys_str_mv | AT gaellebrideau claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT lydiecheval claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT camillegriveau claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT wungmanevelyneling claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT loiclievre claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT gillescrambert claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT dominikmuller claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT jovanabrocic claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT emelinecherchame claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT pascalhouillier claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells AT carolineprotbertoye claudin10expressionandthegeneexpressionpatternofthickascendinglimbcells |