Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model
S.typhimurium is a gram negative bacterium causing serious enteric or extraintestinal infections in human, cattle and birds. These kinds of infections may lead to some permanent or nonpermanent complications like endophthalmitis, Guillain-Barre syndrome and osteomyelitis . Multi-resistant strains of...
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| Format: | Article |
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Kerman University of Medical Sciences
2005-09-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_32756_971af08c9cb5677be63d99dda15c2e19.pdf |
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| author | N.H Jazani Gh Behzadiannejad Sh. Shahabi |
| author_facet | N.H Jazani Gh Behzadiannejad Sh. Shahabi |
| author_sort | N.H Jazani |
| collection | DOAJ |
| description | S.typhimurium is a gram negative bacterium causing serious enteric or extraintestinal infections in human, cattle and birds. These kinds of infections may lead to some permanent or nonpermanent complications like endophthalmitis, Guillain-Barre syndrome and osteomyelitis . Multi-resistant strains of S.typhimurium have been reported from several countries, and these infections are a major public health problem throughout the world. The aim of the present study was to develop a conjugate vaccine for prevention of S.typhimurium infections. For this purpose, polysaccharide side chain was conjugated with tetanus toxoid by carbodiimid mediated amidation method. Immunodiffusion was performed in agarose gel. Four groups of BALB/c mice were selected and immunized with heat killed S.typhimurium, purified polysaccharide, polysaccharide side chain-tetanus toxoid conjugate and sterilized distilled water and the role of each antigen in protection against infection with pathogenic S.typhimurium were investigated. Animals were challenged by intraperitoneal injections. The 50% lethal dose (LD50) was determined on the 21st post challenge day by Reed and Muench method. Immunodiffusion results showed that the conjugate reacted with heat killed whole cell vaccine antiserum and tetanus toxoid antiserum. Three injections of conjugate caused protection against intraperitoneal challenge. In conjugate immunized group LD50 increased 15.4 and 11.11 fold in comparison to control group and polysaccharide side chain immunized group respectively. Results showed that polysaccharide side chain-tetanus toxoid conjugate injected in 3 doses is very protective in mice and can increase the LD50 of immunized animals significantly in comparison to both O antigen vaccinated and control groups. |
| first_indexed | 2024-03-13T02:07:18Z |
| format | Article |
| id | doaj.art-966b4cc94e0d441ebf6b6bfc93ee4977 |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:07:18Z |
| publishDate | 2005-09-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-966b4cc94e0d441ebf6b6bfc93ee49772023-07-01T05:41:56ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432005-09-0111421922732756Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse ModelN.H Jazani0Gh Behzadiannejad1Sh. Shahabi2Assistant Professor, Microbiology Department, Medical School, Orumieh University of Medical Sciences and Health Services, Orumieh, IranAssociate Professor, Microbiology Department,PhD. Student of Immunology, Tarbiat Modares University, Tehran, IranS.typhimurium is a gram negative bacterium causing serious enteric or extraintestinal infections in human, cattle and birds. These kinds of infections may lead to some permanent or nonpermanent complications like endophthalmitis, Guillain-Barre syndrome and osteomyelitis . Multi-resistant strains of S.typhimurium have been reported from several countries, and these infections are a major public health problem throughout the world. The aim of the present study was to develop a conjugate vaccine for prevention of S.typhimurium infections. For this purpose, polysaccharide side chain was conjugated with tetanus toxoid by carbodiimid mediated amidation method. Immunodiffusion was performed in agarose gel. Four groups of BALB/c mice were selected and immunized with heat killed S.typhimurium, purified polysaccharide, polysaccharide side chain-tetanus toxoid conjugate and sterilized distilled water and the role of each antigen in protection against infection with pathogenic S.typhimurium were investigated. Animals were challenged by intraperitoneal injections. The 50% lethal dose (LD50) was determined on the 21st post challenge day by Reed and Muench method. Immunodiffusion results showed that the conjugate reacted with heat killed whole cell vaccine antiserum and tetanus toxoid antiserum. Three injections of conjugate caused protection against intraperitoneal challenge. In conjugate immunized group LD50 increased 15.4 and 11.11 fold in comparison to control group and polysaccharide side chain immunized group respectively. Results showed that polysaccharide side chain-tetanus toxoid conjugate injected in 3 doses is very protective in mice and can increase the LD50 of immunized animals significantly in comparison to both O antigen vaccinated and control groups.https://jkmu.kmu.ac.ir/article_32756_971af08c9cb5677be63d99dda15c2e19.pdfs.typhimuriumlipopolysaccharideconjugate vaccine |
| spellingShingle | N.H Jazani Gh Behzadiannejad Sh. Shahabi Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model Journal of Kerman University of Medical Sciences s.typhimurium lipopolysaccharide conjugate vaccine |
| title | Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model |
| title_full | Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model |
| title_fullStr | Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model |
| title_full_unstemmed | Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model |
| title_short | Production and Investigation of the Effects of Polysaccharide-Protein Conjugate Vaccine in Prevention of Salmonella Typhimurium Infections in Mouse Model |
| title_sort | production and investigation of the effects of polysaccharide protein conjugate vaccine in prevention of salmonella typhimurium infections in mouse model |
| topic | s.typhimurium lipopolysaccharide conjugate vaccine |
| url | https://jkmu.kmu.ac.ir/article_32756_971af08c9cb5677be63d99dda15c2e19.pdf |
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