Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop
Prairie cordgrass ( Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of p...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2010-09-01
|
| Series: | The Plant Genome |
| Online Access: | https://dl.sciencesocieties.org/publications/tpg/articles/3/2/69 |
| _version_ | 1818149474606776320 |
|---|---|
| author | Kristene Gedye Jose Gonzalez-Hernandez Yuguang Ban Xijin Ge Jyothi Thimmapuram Fengjie Sun Chris Wright Shahjahan Ali Arvid Boe Vance Owens |
| author_facet | Kristene Gedye Jose Gonzalez-Hernandez Yuguang Ban Xijin Ge Jyothi Thimmapuram Fengjie Sun Chris Wright Shahjahan Ali Arvid Boe Vance Owens |
| author_sort | Kristene Gedye |
| collection | DOAJ |
| description | Prairie cordgrass ( Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST–SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection. |
| first_indexed | 2024-12-11T13:07:37Z |
| format | Article |
| id | doaj.art-966e2b648c9342d8b03afdb0c272eebd |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-12-11T13:07:37Z |
| publishDate | 2010-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-966e2b648c9342d8b03afdb0c272eebd2022-12-22T01:06:16ZengWileyThe Plant Genome1940-33722010-09-0132698010.3835/plantgenome2010.06.001269Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass CropKristene GedyeJose Gonzalez-HernandezYuguang BanXijin GeJyothi ThimmapuramFengjie SunChris WrightShahjahan AliArvid BoeVance OwensPrairie cordgrass ( Bosc ex Link) is being developed as a cellulosic biomass crop. Development of this species will require numerous steps, including breeding, agronomy, and characterization of the species genome. The research in this paper describes the first investigation of the transcriptome of prairie cordgrass via Next Generation Sequencing Technology, 454 GS FLX. A total of 556,198 expressed sequence tags (ESTs) were produced from four prairie cordgrass tissues: roots, rhizomes, immature inflorescence, and hooks. These ESTs were assembled into 26,302 contigs and 71,103 singletons. From these data were identified, EST–SSR (simple sequence repeat) regions and cell wall biosynthetic pathway genes suitable for the development of molecular markers which can aid the breeding process of prairie cordgrass by means of marker assisted selection.https://dl.sciencesocieties.org/publications/tpg/articles/3/2/69 |
| spellingShingle | Kristene Gedye Jose Gonzalez-Hernandez Yuguang Ban Xijin Ge Jyothi Thimmapuram Fengjie Sun Chris Wright Shahjahan Ali Arvid Boe Vance Owens Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop The Plant Genome |
| title | Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop |
| title_full | Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop |
| title_fullStr | Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop |
| title_full_unstemmed | Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop |
| title_short | Investigation of the Transcriptome of Prairie Cord Grass, a New Cellulosic Biomass Crop |
| title_sort | investigation of the transcriptome of prairie cord grass a new cellulosic biomass crop |
| url | https://dl.sciencesocieties.org/publications/tpg/articles/3/2/69 |
| work_keys_str_mv | AT kristenegedye investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT josegonzalezhernandez investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT yuguangban investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT xijinge investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT jyothithimmapuram investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT fengjiesun investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT chriswright investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT shahjahanali investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT arvidboe investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop AT vanceowens investigationofthetranscriptomeofprairiecordgrassanewcellulosicbiomasscrop |