Two-parameter single-molecule analysis for measurement of chromatin mobility
Summary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302100 |
| _version_ | 1818600948590706688 |
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| author | Jonathan Lerner Pablo Aurelio Gómez-García Ryan L. McCarthy Zhe Liu Melike Lakadamyali Kenneth S. Zaret |
| author_facet | Jonathan Lerner Pablo Aurelio Gómez-García Ryan L. McCarthy Zhe Liu Melike Lakadamyali Kenneth S. Zaret |
| author_sort | Jonathan Lerner |
| collection | DOAJ |
| description | Summary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not.For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020). |
| first_indexed | 2024-12-16T12:43:36Z |
| format | Article |
| id | doaj.art-966ead73821146d3a315874c42706be8 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-16T12:43:36Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-966ead73821146d3a315874c42706be82022-12-21T22:31:21ZengElsevierSTAR Protocols2666-16672020-12-0113100223Two-parameter single-molecule analysis for measurement of chromatin mobilityJonathan Lerner0Pablo Aurelio Gómez-García1Ryan L. McCarthy2Zhe Liu3Melike Lakadamyali4Kenneth S. Zaret5Institute for Regenerative Medicine and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USA; Corresponding authorCentre for Genomic Regulation (CRG), Barcelona Biomedical Research Park, 08003 Barcelona, Spain; The Institute of Photonics Sciences (ICFO), Barcelona Institute of Science and Technology (BIST), 08003 Barcelona, SpainInstitute for Regenerative Medicine and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USAHHMI Janelia Research Campus, Ashburn, VA 20147, USAInstitute for Regenerative Medicine and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USA; University of Pennsylvania, Department of Physiology, Philadelphia, PA 19104-6058, USAInstitute for Regenerative Medicine and Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6058, USASummary: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not.For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720302100Single-molecule assaysMicroscopyMolecular biology |
| spellingShingle | Jonathan Lerner Pablo Aurelio Gómez-García Ryan L. McCarthy Zhe Liu Melike Lakadamyali Kenneth S. Zaret Two-parameter single-molecule analysis for measurement of chromatin mobility STAR Protocols Single-molecule assays Microscopy Molecular biology |
| title | Two-parameter single-molecule analysis for measurement of chromatin mobility |
| title_full | Two-parameter single-molecule analysis for measurement of chromatin mobility |
| title_fullStr | Two-parameter single-molecule analysis for measurement of chromatin mobility |
| title_full_unstemmed | Two-parameter single-molecule analysis for measurement of chromatin mobility |
| title_short | Two-parameter single-molecule analysis for measurement of chromatin mobility |
| title_sort | two parameter single molecule analysis for measurement of chromatin mobility |
| topic | Single-molecule assays Microscopy Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720302100 |
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