Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes
<p>Abstract</p> <p>Background</p> <p>The yeast cell wall integrity mitogen-activated protein kinase (CWI-MAPK) pathway is the main regulator of adaptation responses to cell wall stress in yeast. Here, we adopt a genomic approach to shed light on two aspects that are onl...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-08-01
|
| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/12/390 |
| _version_ | 1818750779601715200 |
|---|---|
| author | Arias Patricia Díez-Muñiz Sonia García Raúl Nombela César Rodríguez-Peña José M Arroyo Javier |
| author_facet | Arias Patricia Díez-Muñiz Sonia García Raúl Nombela César Rodríguez-Peña José M Arroyo Javier |
| author_sort | Arias Patricia |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The yeast cell wall integrity mitogen-activated protein kinase (CWI-MAPK) pathway is the main regulator of adaptation responses to cell wall stress in yeast. Here, we adopt a genomic approach to shed light on two aspects that are only partially understood, namely, the characterization of the gene functional catalog associated with CWI pathway activation and the extent to which MAPK activation correlates with transcriptional outcomes.</p> <p>Results</p> <p>A systematic yeast mutant deletion library was screened for constitutive transcriptional activation of the CWI-related reporter gene <it>MLP1</it>. Monitoring phospho-Slt2/Mpk1 levels in the identified mutants revealed sixty-four deletants with high levels of phosphorylation of this MAPK, including mainly genes related to cell wall construction and morphogenesis, signaling, and those with unknown function. Phenotypic analysis of the last group of mutants suggests their involvement in cell wall homeostasis. A good correlation between levels of Slt2 phosphorylation and the magnitude of the transcriptional response was found in most cases. However, the expression of CWI pathway-related genes was enhanced in some mutants in the absence of significant Slt2 phosphorylation, despite the fact that functional MAPK signaling through the pathway was required. CWI pathway activation was associated to increased deposition of chitin in the cell wall - a known survival compensatory mechanism - in about 30% of the mutants identified.</p> <p>Conclusion</p> <p>We provide new insights into yeast genes related to the CWI pathway and into how the state of activation of the Slt2 MAPK leads to different outcomes, discovering the versatility of this kind of signaling pathways. These findings potentially have broad implications for understanding the functioning of other eukaryotic MAPKs.</p> |
| first_indexed | 2024-12-18T04:25:06Z |
| format | Article |
| id | doaj.art-9670ae3a31de4e46a98d2d2f9d35f630 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T04:25:06Z |
| publishDate | 2011-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-9670ae3a31de4e46a98d2d2f9d35f6302022-12-21T21:21:07ZengBMCBMC Genomics1471-21642011-08-0112139010.1186/1471-2164-12-390Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomesArias PatriciaDíez-Muñiz SoniaGarcía RaúlNombela CésarRodríguez-Peña José MArroyo Javier<p>Abstract</p> <p>Background</p> <p>The yeast cell wall integrity mitogen-activated protein kinase (CWI-MAPK) pathway is the main regulator of adaptation responses to cell wall stress in yeast. Here, we adopt a genomic approach to shed light on two aspects that are only partially understood, namely, the characterization of the gene functional catalog associated with CWI pathway activation and the extent to which MAPK activation correlates with transcriptional outcomes.</p> <p>Results</p> <p>A systematic yeast mutant deletion library was screened for constitutive transcriptional activation of the CWI-related reporter gene <it>MLP1</it>. Monitoring phospho-Slt2/Mpk1 levels in the identified mutants revealed sixty-four deletants with high levels of phosphorylation of this MAPK, including mainly genes related to cell wall construction and morphogenesis, signaling, and those with unknown function. Phenotypic analysis of the last group of mutants suggests their involvement in cell wall homeostasis. A good correlation between levels of Slt2 phosphorylation and the magnitude of the transcriptional response was found in most cases. However, the expression of CWI pathway-related genes was enhanced in some mutants in the absence of significant Slt2 phosphorylation, despite the fact that functional MAPK signaling through the pathway was required. CWI pathway activation was associated to increased deposition of chitin in the cell wall - a known survival compensatory mechanism - in about 30% of the mutants identified.</p> <p>Conclusion</p> <p>We provide new insights into yeast genes related to the CWI pathway and into how the state of activation of the Slt2 MAPK leads to different outcomes, discovering the versatility of this kind of signaling pathways. These findings potentially have broad implications for understanding the functioning of other eukaryotic MAPKs.</p>http://www.biomedcentral.com/1471-2164/12/390 |
| spellingShingle | Arias Patricia Díez-Muñiz Sonia García Raúl Nombela César Rodríguez-Peña José M Arroyo Javier Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes BMC Genomics |
| title | Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes |
| title_full | Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes |
| title_fullStr | Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes |
| title_full_unstemmed | Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes |
| title_short | Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes |
| title_sort | genome wide survey of yeast mutations leading to activation of the yeast cell integrity mapk pathway novel insights into diverse mapk outcomes |
| url | http://www.biomedcentral.com/1471-2164/12/390 |
| work_keys_str_mv | AT ariaspatricia genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes AT diezmunizsonia genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes AT garciaraul genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes AT nombelacesar genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes AT rodriguezpenajosem genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes AT arroyojavier genomewidesurveyofyeastmutationsleadingtoactivationoftheyeastcellintegritymapkpathwaynovelinsightsintodiversemapkoutcomes |